Endothelial viability of organ-cultured corneas following penetrating keratoplasty

Harper, C L; Boulton, Michael E.; Marcyniuk, B.; Tullo, Andrew B.; Ridgway, A E A

doi:10.1038/eye.1998.214

Cited by 14 publications

(10 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, we found −28% of ECs at D5, −33% at D15, reaching −42% at M3, percentages that are rather in the range of the typically accepted cell loss at 6 months (−28.8% for Bertelmann et al )29 or even 1 year in normal-risk PK after OC (−39.4% for Harper et al ,30 or −29.7% for Borderie et al ) 2. Looking ECD, instead of cell loss, of different series of PK performed with OC corneas, they were substantially and systematically lower for our patients.…”

Section: Discussioncontrasting

confidence: 49%

Very early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas

et al. 2016

View full text Add to dashboard Cite

Aims After keratoplasty, postoperative endothelial cell loss is calculated between the eye bank endothelial cell density (ebECD) and the postoperative specular microscopy (SM). To elucidate the very early cell loss, always described after penetrating keratoplasty (PK), we designed two complementary studies. Methods (1) Clinical prospective study of 90 consecutive PKs (keratoconus, Fuchs' corneal dystrophy, lattice dystrophy, bullous keratopathy) with organ-cultured corneas and postoperative follow-up by SM at day 5 (D5), D15, month 1 (M1) and M3. This series provided a quantification of the difference between ebECD performed 2 days before graft and very early postoperative ECD. (2) Ten pairs of corneas with comparable ebECD in both corneas and same organ-culture (OC) duration were randomised: one cornea was grafted, and, at the same time, the viable ECD (vECD) of the other was measured after labelling with Hoechst/ethidium/calcein-AM. The relationship between vECD and very early postoperative ECD was studied.Results vECD at the time of graft did not differ from ECD 5 days after PK, with a difference of 39 (−356; 355) cells/mm 2 (median (10°; 90°percentile, p=0.799)), whereas a significant difference of 755 (359; 1146) cells/mm 2 , corresponding to 28% (95% CI 26 to 30) of cells, was measured between ebECD and ECD 5 days after PK ( p<0.001). Conclusions In OC, ebECD provided to surgeons significantly overestimate the number of viable ECs grafted to patients. The absence of difference between the vECD at D0 and ECD at D5 indicates that the very early endothelial cell loss is almost negligible in recipients.

show abstract

Section: Discussioncontrasting

confidence: 49%

Very early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas

et al. 2016

View full text Add to dashboard Cite

show abstract

“…7 The postoperative endothelial cell loss reported in donor corneas stored by this method is 8% at 2 months 8 and 19% at 3 months. 9 The preoperative baseline endothelial cell densities for these studies, however, were measured at the end of the storage period, and reported losses do not include the loss during storage, which has been found by many investigators to be 10% or more. [10][11][12][13][14][15] …”

Section: Donor Preservationmentioning

confidence: 99%

Cellular Changes in Transplanted Human Corneas

Bourne¹

2001

Cornea

235

135

View full text Add to dashboard Cite

show abstract

“…Recently, a new organ culture system has been developed that allowed to easily and reproducibly culture corneas at the air-liquid interface on top of the collagen-agar layer (Foreman et al, 1996;Xu et al, 2000;Zieske et al, 2000). This system was chosen here because (1) it reproduced well the process of normal wound healing, and (2) corneas could be successfully transplanted to patients after long-term culture with this technique (Harper et al, 1998). It is shown here that normal and DR organ-cultured corneas preserve their in vivo differences in respect to rates of wound healing and diabetic corneal markers distribution.…”

Section: Introductionmentioning

confidence: 99%

Human diabetic corneas preserve wound healing, basement membrane, integrin and MMP-10 differences from normal corneas in organ culture

Kabosova

Kramerov

Aoki

et al. 2003

Experimental Eye Research

View full text Add to dashboard Cite

The authors have previously documented decreased epithelial basement membrane (BM) components and alpha3beta1 epithelial integrin, and increased expression of matrix metalloproteinase (MMP)-10 in corneas of patients with diabetic retinopathy (DR) compared to normal corneas. The purpose of this study was to examine if organ-cultured DR corneas exhibited the same alterations in wound healing and diabetic marker distribution as the autopsy DR corneas. Twenty normal and 17 DR corneas were organ-cultured in serum-free medium over agar-collagen gel at the air-liquid interface for up to 45 days. Circular 5 mm central epithelial wounds were made with n-heptanol, the procedure that will preserve fragile diabetic corneal BM. Wound healing was monitored microscopically every 12 hr. Distribution of diabetic corneal epithelial markers including laminin-10 alpha5 chain, nidogen-1/entactin, integrin alpha3beta1, and MMP-10, was examined by immunofluorescence. Normal corneas healed the central epithelial defect within 3 days (mean=2.3 days), whereas DR corneas on average healed about two times slower (mean=4.5 days). In wounded and completely healed organ-cultured corneas, the patterns of studied markers were the same as in the unwounded organ-cultured corneas. This concerned both normal and DR corneas. As in vivo, normal organ-cultured corneas had continuous staining for laminin-10 and nidogen-1/entactin in the epithelial BM, strong and homogeneous staining for both chains of alpha3beta1 integrin in epithelial cells, and little if any staining for MMP-10. Organ-cultured DR corneas also had marker patterns specific for in vivo DR corneas: interrupted to no staining for laminin-10 and nidogen-1/entactin in the epithelial BM, areas of weak or disorganized alpha3beta1 integrin in epithelial cells, and significant MMP-10 staining in the epithelium and keratocytes. Fibrotic extracellular matrix and myofibroblast markers were largely absent. Thus, epithelial wound healing was much slower in organ-cultured DR corneas than in normal corneas, in complete accordance with clinical data in diabetic patients. DR corneas in organ culture preserved the same marker abnormalities as in vivo. The marker distribution was unchanged in wounded and healed organ-cultured corneas, compared to unwounded corneas. The established corneal organ culture provides an adequate system for elucidating mechanisms of epithelial alterations in human DR corneas.

show abstract

Endothelial viability of organ-cultured corneas following penetrating keratoplasty

Cited by 14 publications

References 18 publications

Very early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas

Very early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas

Cellular Changes in Transplanted Human Corneas

Human diabetic corneas preserve wound healing, basement membrane, integrin and MMP-10 differences from normal corneas in organ culture

Contact Info

Product

Resources

About