Endoscope-tip interferometer for ultrahigh resolution frequency domain optical coherence tomography in mouse colon

Tumlinson, Alexandre R.; Barton, Jennifer K.; Považay, Boris; Sattman, Harald; Unterhuber, Angelika; Leitgeb, Rainer A.; Drexler, Wolfgang

doi:10.1364/oe.14.001878

Cited by 91 publications

(63 citation statements)

References 31 publications

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“…As a result, there has been great interest in improving the resolution of OCT by an order of magnitude along each dimension to enable visualization of abnormal cells in living patients [4]. Near-isotropic μm-resolution forms of OCT have been termed ultra-high-resolution OCT (UHR-OCT) or μOCT in literatures [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

μOCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe

et al. 2016

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Optical coherence tomography (OCT) is an attractive medical modality due to its ability to acquire high-resolution, cross-sectional images inside the body using flexible, small-diameter, scanning fiber optic probes. Conventional, cross-sectional OCT imaging technologies have approximately 10-μm axial resolution and 30-μm lateral resolution, specifications that enable the visualization of microscopic architectural morphology. While this resolution is useful for many clinical applications, it is insufficient for resolving individual cells that characterize many diseases. To address this gap, a supercontinuum-laser-based, μm-resolution OCT (μOCT) system and a 500 μm-diameter, extended depth of focus single fiber optic probe for endoscopic and intravascular imaging were designed and fabricated. At the distal tip of the fiber optic probe, a cylindrical waveguide was used to divide the wavefront to provide multiple circular propagation modes. Once transmitted through a relatively high NA lens (NA >0.1), these modes were projected as multiple coaxial foci (~3 μm full width at half maximum (FWHM)) over a greatly extended focal depth range. The distal tip of the probe also contained a common-path reference reflectance to minimize polarization and dispersion imbalances between sample and reference arm light. Measurements showed that the probe provides a 20-fold depth of focus extension, maintaining a 3-5 µm lateral resolution (FWHM of PSF) and a 2 μm axial resolution over a depth range of approximately 1 mm. These results suggest that this new optical configuration will be useful for achieving high-resolution, cross-sectional OCT imaging in catheter/endoscope-based medical imaging devices.

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Section: Introductionmentioning

confidence: 99%

μOCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe

et al. 2016

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“…Combining these two complementary imaging technologies would potentially improve the diagnostic capability for early cancer detection. Previous studies using OCT and depth-integrated fluorescence imaging already showed substantial promises in cervical [30,31], bladder [29,32], colorectal [33][34][35], and bronchial cancers [36]. However, depth-integrated fluorescence imaging lacks the depth-resolved information, therefore may not sufficiently indicate the subsurface lesions or estimate the depth-extent of the lesions.…”

Section: Introductionmentioning

confidence: 99%

Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

Tang

Wang

Frank

et al. 2016

Biomed. Opt. Express

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Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc Min /J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization. Muthasamy, C. J. Lightdale, N. Santiago, D. K. Pleskow, P. J. Dean, and K. K. Wang, "Endoscopic radiofrequency ablation for Barrett's esophagus: 5-year outcomes from a prospective multicenter trial," Endoscopy 42(10), 781-789 (2010 cancer lesion with swept-source optical coherence tomography," J.

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“…However, this approach compromises co-registration of the two modalities. Other reported approaches have used single Ti-Sapphire broadband femtosecond (fs) laser sources for both spectral domain OCT and multiphoton excited fluorescence imaging systems [25][26][27][28][29][30][31][32]. Double-clad fiber (DCF) can also provide a common path for the two modalities that ensures co-registration.…”

Section: Introductionmentioning

confidence: 99%

A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

Pahlevaninezhad¹,

Lee²,

Shaipanich³

et al. 2014

Biomed. Opt. Express

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Abstract:We present a power-efficient fiber-based imaging system capable of co-registered autofluorescence imaging and optical coherence tomography (AF/OCT). The system employs a custom fiber optic rotary joint (FORJ) with an embedded dichroic mirror to efficiently combine the OCT and AF pathways. This three-port wavelength multiplexing FORJ setup has a throughput of more than 83% for collected AF emission, significantly more efficient compared to previously reported fiber-based methods. A custom 900 µm diameter catheter -consisting of a rotating lens assembly, double-clad fiber (DCF), and torque cable in a stationary plastic tube -was fabricated to allow AF/OCT imaging of small airways in vivo. We demonstrate the performance of this system ex vivo in resected porcine airway specimens and in vivo in human on fingers, in the oral cavity, and in peripheral airways. 36. S. Liang, A. Saidi, J. Jing, G. Liu, J. Li, J. Zhang, C. Sun, J. Narula, and Z. Chen, "Intravascular atherosclerotic imaging with combined fluorescence and optical coherence tomography probe based on a double-clad fiber combiner," J.

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Endoscope-tip interferometer for ultrahigh resolution frequency domain optical coherence tomography in mouse colon

Cited by 91 publications

References 31 publications

μOCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe

μOCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe

Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

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