Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography

Abstract

Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc Min /J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization. Muthasamy, C. J. Lightdale, N. Santiago, D. K. Pleskow, P. J. Dean, and K. K. Wang, "Endoscopic radiofrequency ablation for Barrett's esophagus: 5-year outcomes from a prospective multicenter trial," Endoscopy 42(10), 781-789 (2010 cancer lesion with swept-source optical coherence tomography," J.

Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

“…A high-speed, high-resolution Fourier-domain OCT system was used in this study. 10,13,[53][54][55] The details of the OCT system have been previously described. 13 Brie°y, an OCT system consisting of a swept-source laser (100 nm bandwidth at 1310 nm center wavelength) yielding an axial resolution of 10 m in tissue was used.…”

Optical coherence tomography and computer-aided diagnosis of a murine model of chronic kidney disease

“…1). [34][35][36][37] This system uses a swept-source laser operating at 1310-nm center wavelength with 100-nm bandwidth. The axial and lateral resolutions of the system are 12 and 6 μm, respectively.…”

Depth-resolved mapping of muscular bundles in myocardium pulmonary junction using optical coherence tomography

“…The details of the SS-OCT system have been previously described. [13][14][15][16][17] Briefly, the light source was a swept-source laser with 100-nm bandwidth centered at 1310 nm, which yielded an axial resolution of ∼10 μm in tissue. The lateral resolution was ∼15 μm.…”