A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

Abstract

Abstract:We present a power-efficient fiber-based imaging system capable of co-registered autofluorescence imaging and optical coherence tomography (AF/OCT). The system employs a custom fiber optic rotary joint (FORJ) with an embedded dichroic mirror to efficiently combine the OCT and AF pathways. This three-port wavelength multiplexing FORJ setup has a throughput of more than 83% for collected AF emission, significantly more efficient compared to previously reported fiber-based methods. A custom 900 µm diameter catheter -consisting of a rotating lens assembly, double-clad fiber (DCF), and torque cable in a stationary plastic tube -was fabricated to allow AF/OCT imaging of small airways in vivo. We demonstrate the performance of this system ex vivo in resected porcine airway specimens and in vivo in human on fingers, in the oral cavity, and in peripheral airways. 36. S. Liang, A. Saidi, J. Jing, G. Liu, J. Li, J. Zhang, C. Sun, J. Narula, and Z. Chen, "Intravascular atherosclerotic imaging with combined fluorescence and optical coherence tomography probe based on a double-clad fiber combiner," J.

The potential role of in vivo optical coherence tomography for evaluating oral soft tissue: A systematic review

“…Twelve articles described OCT findings regarding healthy oral mucosa. Ridgway et al showed how the OCT image of normal buccal mucosa allows identification of stratified squamous epithelium (SS) along the mucosal surface, the underlying lamina propria (LP), and the transition between these tissues along the basement membrane (BM) boundary.…”

“…However, based on the same basic principles, different devices allow inspection of the tissues with different parameters and different techniques, making comparisons among results obtained by the various research groups difficult. In 13 of the 27 studies, the oral mucosa was examined with a commercial OCT apparatus (seven different types of equipment); the remaining 14 used an OCT system developed by the authors.…”

Method for single illumination source combined optical coherence tomography and fluorescence imaging of fluorescently labeled ocular structures in transgenic mice

“…Conventional multi-modality systems require multiple sources which increase complexity of the system [4, 9, 20, 21]. Additionally both sources contribute to the ocular maximum permissible exposure (MPE) [14, 15].…”

En-face optical coherence tomography/fluorescence endomicroscopy for minimally invasive imaging using a robotic scanner

“…Depending on the kind of tissue being investigated, the imaging contrast provided by OCT might not be sufficient for clinical diagnosis, since it stems solely from single backscattering events and changes in the refractive index. Therefore, it is recommendable to combine OCT with other optical and nonoptical modalities, [7][8][9][10][11] to enable more comprehensive characterization of the tissue properties.…”