Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand

Abstract

Species of Colletotrichum are commonly found in many plant hosts as pathogens, endophytes and occasionally saprobes. Twenty-two Colletotrichum strains were isolated from three Dendrobium species – D.cariniferum, D.catenatum and D.harveyanum, as well as three unidentified species. The taxa were identified using morphological characterisation and phylogenetic analyses of ITS, GAPDH, ACT and ß–tubulin sequence data. This is the first time to identify endophytic fungi from Dendrobium orchids using the above method. The known species, Colletotrichumboninense, C.camelliae-japonicae, C.fructicola, C.jiangxiense and C.orchidophilum were identified as fungal endophytes of Dendrobium spp., along with the new species, C.cariniferi, C.chiangraiense, C.doitungense, C.parallelophorum and C.watphraense, which are introduced in this paper. One strain is recorded as an unidentified species. Corn meal agar is recommended as a good sporulation medium for Colletotrichum species. This is the first report of fungal endophytes associated with Dendrobiumcariniferum and D.harveyanum. Colletotrichumcamelliae-japonicae, C.jiangxiense, and C.orchidophilum are new host records for Thailand.

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

“…Colletotrichum species can be found as pathogens, endophytes, and saprobes in a variety of plant hosts. Ma et al [12] isolated endophytic Colletotrichum from Dendrobium spp. in China and Northern Thailand.…”

Multigene Phylogeny Reveals Endophytic Xylariales Novelties from Dendrobium Species from Southwestern China and Northern Thailand

“…Therefore, applying the culture-dependent method or HTS only maybe not reveal the real composition of fungal endophytes. It is likely that combining both methods or improving the HTS primers can contribute to discovering the real composition, taxonomic placements, and ecological strategies [ 125 , 126 , 127 ]. Several advanced metabarcoding strategies and platforms have been introduced to achieve species identification in dietary, gut microbiome, and wildlife forensic species detection, which is likely to be used for more effective and exact fungal endophytes identification [ 128 ].…”

Colletotrichum siamense, a Mycovirus-Carrying Endophyte, as a Biological Control Strategy for Anthracnose in Guarana Plants

“…Colletotrichum species can be found in nature as pathogens, endophytes, and sometimes as saprophytes [30]. Both C. siamense and C. fructicola have already been isolated as phytopathogens [31,32] and as endophytes in different hosts [33,34]. The behavior of Colletotrichum species is known to be highly regulated by specific gene families and biochemical interactions that occur through specific enzymes and secondary metabolites produced at the host-pathogen interface.…”