Endocrine-active compound evaluation: Qualitative and quantitative histomorphological assessment of zebrafish gonads after bisphenol-A exposure

Molina, A.; Lora, Antonio; Blanco, Alfonso; Monterde, J. G.; Ayala, Nahúm; Moyano, Rosario

doi:10.1016/j.ecoenv.2012.11.010

Cited by 47 publications

(36 citation statements)

References 23 publications

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“…In agreement with previously reported data, no mortalities occurred during the BPA exposure period (Hatef et al 2012;Villaneuve et al 2012), nor were there any differences with regard to the weight and length of the animals after the study period. These data are in accordance with those previously reported by Mandich et al (2007) and with our own earlier study (Molina et al 2013). However, the macroscopic aspect of the gonads remained apparently normal in our study, whereas other authors, after a period of exposure to 17-β-oestradiol, noted an attenuated appearance in sperm ducts (Wolf et al 2004).…”

Section: Discussionsupporting

confidence: 83%

“…1, 10, 100 and 1000 µg/l, was achieved by means of a continuous flow system in the water of the aquariums. These doses are very similar to those previously employed by different authors in diverse fish species (Ishibashi et al 2005;Mandich et al 2007;Villaneuve et al 2012;Molina et al 2013). In agreement with previously reported data, no mortalities occurred during the BPA exposure period (Hatef et al 2012;Villaneuve et al 2012), nor were there any differences with regard to the weight and length of the animals after the study period.…”

Section: Discussionsupporting

confidence: 70%

“…Zebrafish are one of a group of small fish species that can be kept in the laboratory as they are easily exposed to endocrine-disrupting chemicals in tank water at different stages in their life cycle, and exhibit a measurable sensitivity to such agents ( Van den Belt et al 2001;Orn et al 2003;McGonnell and Fowkes 2006;Molina et al 2013).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods

Lora¹,

Molina²,

Bellido³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The purpose of this study was to evaluate the consequences of bisphenol A (BPA) exposure in zebrafish (Danio rerio). Short-term effects at a testes level were assessed by means of different biomarkers in 60 male zebrafish exposed for 14 days in aquariums to different concentrations of BPA (1, 10, 100 and 1000 µg/l) in addition to a control batch. The LC-MS/MS method was used for the quantification of BPA. The BPA levels in the zebrafish tissues increased together with increasing exposure of fish to BPA. Several alterations were observed in the zebrafish testes. Particularly notable morphological changes included, a pronounced degeneration of all cell components, an increase in the percentage of the sustentacular (Sertoli) cells, and a marked decrease in the percentage of germ cells starting from concentrations of 100 µg/l of BPA. Our results indicate that histopathology and testes morphometry are sensitive biomarkers for the analysis of early effects of an environmental concentration of BPA in zebrafish experimental models. Our observations reveal that the nature and intensity of the morphological changes in the testes are concentration-dependent in the case of BPA, and manifest as adverse effects on the fish's reproductive health.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 70%

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Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods

Lora¹,

Molina²,

Bellido³

et al. 2016

Vet. Med. - Czech

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“…The low dose cut-off of 1 × 10 -7 M has been debated and it may be reasonable to shift the low dose cut-off for in vitro studies to 10 One final study deserves attention due to its use of Organization for Economic Cooperation and Development (OECD) guidelines to test the effects of BPA on histopathological changes in the ovary. 112 Zebrafish were exposed to very low doses of BPA for 2 wk starting at 16 wk of age; histological abnormalities were observed at 1, 10, 100, and 1000 μg/L, providing strong evidence for low dose adverse effects on the fish ovary. OECD guidelines examine endpoints that are easily assessed, are reproducible, and focus on adverse effects, 113 although there is debate regarding whether they examine appropriate and sensitive endpoints for endocrine disruption.…”

Section: -53mentioning

confidence: 99%

Low dose effects of bisphenol A

Vandenberg

Ehrlich

Belcher

et al. 2013

Endocrine Disruptors

182

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“…We have also seen how BPa triggers important effects on ovaries and testicles, which could interfere with fertility (MOLINa et al, 2013;LOra et al, 2016), in addition to modifying other systems, such as the musculoskeletal system (gIMéNez et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Histopathological study of the Achilles tendon after exposure to bisphenol-A and its subsequent treatment with platelet growth factors

Bellido¹,

Córdoba²,

Abellán³

et al. 2018

Vet. arhiv

Self Cite

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Bisphenol-a is a well-known compound with estrogenic activity, which is produced in tons world-wide and is abundantly used as a component of food containers, so that it frequently migrates into food and appears as an environmental pollutant. this study aimed to assess the effects of Bisphenol-a at the tendon level, possible reversal after it is withdrawn from exposure, and its recovery after the application of plate-enriched plasma. For this purpose, 16 animals were randomly distributed to one of the experiment groups: control group, group treated with BPa (for 4 months), group BPa-withdrawal (treated for 2 months with BPa and two months without BPa), and platelet-rich plasma (PrP) group (treated for 2 months with BPa, when it was withdrawn and the PrP treatment began for 2 months). at the end of the treatments biopsy of the achilles tendon was carried out. the samples were analysed by optical and electron microscopy and immunohistochemistry. histopathology was a fundamental tool in the evaluation of the tendon after exposure to Bisphenol-a. Our results showed how, after withdrawal of BPa, there was recovery from the lesions caused, with diminution in the type III collagen fibres along with tendon vascularization processes; in the group to which PRP was applied, almost complete recovery from the lesions caused occurred, and the immunocytochemistry test was negative for the type III collagen fibres. Our results indicate that tendons were affected after BPA exposure, but that the lesions were partially reversible after BPa was withdrawn. PrP application enabled tendon lesions to recuperate and all signs of inflammation disappeared.

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Endocrine-active compound evaluation: Qualitative and quantitative histomorphological assessment of zebrafish gonads after bisphenol-A exposure

Cited by 47 publications

References 23 publications

Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods

Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods

Low dose effects of bisphenol A

Histopathological study of the Achilles tendon after exposure to bisphenol-A and its subsequent treatment with platelet growth factors

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