Endocan concentrations in postmortem serum, vitreous humor and urine in victims of lethal hypothermia

Descloux, Emilienne; Augsburger, Marc; Teresiński, Grzegorz; Hejna, Petr; Grouzmann, Eric; Scarpelli, Maria Pia; Hervet, Tania; Palmière, Cristian

doi:10.1016/j.jflm.2017.07.002

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Information regarding biochemical markers that have already been studied but have not demonstrated their significance in the exploration of hypothermic deaths include: urinary histamine, urinary serotonin, blood, vitreous and urinary glucose and blood, vitreous and endocan [12,14,61].…”

Section: Other Biomarkersmentioning

confidence: 99%

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

Rousseau

Reynier

Jousset

et al. 2018

Clinical Chemistry and Laboratory Medicine (CCLM)

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Hypothermia is defined as a core body temperature below 35°C and can be caused by environmental exposure, drug intoxication, metabolic or nervous system dysfunction. This lethal pathology with medico-legal implications is complex to diagnose because macroscopic and microscopic lesions observed at the autopsy and the histological analysis are suggestive but not pathognomonic. Postmortem biochemical explorations have been progressively developed through the study of several biomarkers to improve the diagnosis decision cluster. Here, we present an updated review with novel biomarkers (such as catecholamines O-methylated metabolites, thrombomodulin and the cardiac oxyhemoglobin ratio) as well as some propositional interpretative postmortem thresholds and, to the best of our knowledge, for the first time, we present the most adapted strategy of sampling and analyses to identify biomarkers of hypothermia. For our consideration, the most relevant identified biomarkers are urinary catecholamines and their O-methylated metabolites, urinary free cortisol, blood cortisol, as well as blood, vitreous humor and pericardial fluid for ketone bodies and blood free fatty acids. These biomarkers are increased in response either to cold-mediated stress or to bioenergetics ketogenesis crisis and significantly contribute to the diagnosis by exclusion of death by hypothermia.

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Section: Other Biomarkersmentioning

confidence: 99%

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

Rousseau

Reynier

Jousset

et al. 2018

Clinical Chemistry and Laboratory Medicine (CCLM)

Self Cite

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show abstract

“…Several studies have shown potential metabolite biomarkers of hypothermia fatalities in the vitreous humor without any pathognomonic sign. − A decrease of creatinine without significant perturbation of urea has been reported . Increased ketogenesis, related to heat production, was also reported in the vitreous humor in the case of hypothermia fatalities with high levels of hydroxybutyrate, acetone, and isopropyl alcohol .…”

Section: Introductionmentioning

confidence: 99%

Preliminary Metabolomic Profiling of the Vitreous Humor from Hypothermia Fatalities

et al. 2021

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The postmortem diagnosis of hypothermia fatalities is often complex due to the absence of pathognomonic lesions and biomarkers. In this study, potential novel biomarkers of hypothermia fatalities were searched in the vitreous humor of known cases of hypothermia fatalities (n = 20) compared to control cases (n = 16), using a targeted metabolomics approach allowing quantitative detection of 188 metabolites. A robust discriminant model with good predictivity was obtained with the supervised OPLS-DA multivariate analysis, showing a distinct separation between the hypothermia and control groups. This signature was characterized by the decreased concentrations of five metabolites (methionine sulfoxide, tryptophan, phenylalanine, alanine, and ornithine) and the increased concentration of 28 metabolites (21 phosphatidylcholines, 3 sphingomyelins, spermine, citrulline, acetylcarnitine, and hydroxybutyrylcarnitine) in hypothermia fatalities compared to controls. The signature shows similarities with already identified features in serum such as the altered concentrations of tryptophan, acylcarnitines, and unsaturated phosphatidylcholines, revealing a highly significant increased activity of methionine sulfoxide reductase, attested by a low methionine sulfoxide-to-methionine ratio. Our results show a preliminary metabolomics signature of hypothermia fatalities in the vitreous humor, highlighting an increased methionine sulfoxide reductase activity.

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Postmortem diagnosis of fatal hypothermia/hyperthermia by spectrochemical analysis of plasma

Lin

Zou

Luo

et al. 2019

Forensic Sci Med Pathol

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Endocan concentrations in postmortem serum, vitreous humor and urine in victims of lethal hypothermia

Cited by 6 publications

References 28 publications

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

Preliminary Metabolomic Profiling of the Vitreous Humor from Hypothermia Fatalities

Postmortem diagnosis of fatal hypothermia/hyperthermia by spectrochemical analysis of plasma

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