Encapsulation of Amoxicillin within Laponite-Doped Poly(lactic-co-glycolic acid) Nanofibers: Preparation, Characterization, and Antibacterial Activity

Abstract

We report a facile approach to encapsulating amoxicillin (AMX) within laponite (LAP)-doped poly(lactic-co-glycolic acid) (PLGA) nanofibers for biomedical applications. In this study, a synthetic clay material, LAP nanodisks, was first used to encapsulate AMX. Then, the AMX-loaded LAP nanodisks with an optimized AMX loading efficiency of 9.76 ± 0.57% were incorporated within PLGA nanofibers through electrospinning to form hybrid PLGA/LAP/AMX nanofibers. The loading of AMX within LAP nanodisks and the loading of LAP/AMX within PLGA nanofibers were characterized via different techniques. In vitro drug release profile, antimicrobial activity, and cytocompatibility of the formed hybrid PLGA/LAP/AMX nanofibers were also investigated. We show that the loading of AMX within LAP nanodisks does not lead to the change of LAP morphology and crystalline structure and the incorporation of LAP/AMX nanodisks does not significantly change the morphology of the PLGA nanofibers. Importantly, the loading of AMX within LAP-doped PLGA nanofibers enables a sustained release of AMX, much slower than that within a single carrier of LAP nanodisks or PLGA nanofibers. Further antimicrobial activity and cytocompatibility assays demonstrate that the antimicrobial activity of AMX toward the growth inhibition of a model bacterium of Staphylococcus aureus is not compromised after being loaded into the hybrid nanofibers, and the PLGA/LAP/AMX nanofibers display good cytocompatibility, similar to pure PLGA nanofibers. With the sustained release profile and the reserved drug activity, the organic/inorganic hybrid nanofiber-based drug delivery system may find various applications in tissue engineering and pharmaceutical science.

Antitumor activity of electrospun polylactide nanofibers loaded with 5-fluorouracil and oxaliplatin against colorectal cancer

“…The majority of these devices are biodegradable so as to circumvent a second surgery for device removal and to avoid a chronic foreign body immune response (Langer, 1980;Kim et al, 2009b;De Souza et al, 2010;Wolinsky et al, 2012). Among them electrospun fibers are known as an excellent drug carrier by providing large surface area to volume for high drug loading and encapsulation efficiency, and a three-dimensional open porous structure, which can reduce the constraint to drug diffusion leading to an increase in the total fraction of drug that can be released (Qi et al, 2010;Wang et al, 2012;Zheng et al, 2013b). In addition, electrospinning affords great flexibility in producing polymer fibers with customizable fiber size, porosity, drug loading rate and release mechanisms, leading to a possibility of tailoring the drug release rate for each application.…”

Antitumor Activity of Doxorubicin-Loaded Carbon Nanotubes Incorporated Poly(Lactic-Co-Glycolic Acid) Electrospun Composite Nanofibers

“…DOX was firstly loaded It is known that the morphology of electrospun nanofibers is influenced by various parameters, such as the concentration of polymers, applied voltage, and flow rate of solution. For the organic/inorganic composite nanofibers, the morphology and the diameter can be dramatically affected by the content of inorganic component in polymers [20,26]. Therefore, it is encouraged to investigate the effects of the content of CNTs on the nanofibrous morphology and diameter.…”

“…However, the simple introduction of drug into polymer matrix always leads to inevitable burst drug release, since the drug molecules might migrate on or near the fiber surfaces because of the high ionic strength in drug/ polymer solution and the rapid evaporation of the solvent during electrospinning. To address this limitation, it is highly desirable to develop efficient nanocarrier-mediated nanofibrous delivery systems which may serve as barrier for improving the safety of anticancer drugs and avoid drug premature burst release under physiological conditions [7,[17][18][19][20][21]. In fact, several nanoscale carriers such as mesoporous silica nanoparticles [22], hydroxyapatite [10], and liposome [23] have recently been incorporated into electrospun nanofibers for potential anticancer therapy, from which the prolonged drug release with tunable drug release kinetics could be achieved.…”

Investigation of the electro-spinnability of alginate solutions containing gold precursor HAuCl 4

“…Owning a significantly high surface to volume ratio, nanofibers produced by electrospinning can be used in various sectors, including catalysis, sensing, electronics, optics, filtration and biotechnology [1][2][3][4][5]. Electrospinning is an ideal method for generating fibers with diameter in the nano-range.…”