Antitumor activity of electrospun polylactide nanofibers loaded with 5-fluorouracil and oxaliplatin against colorectal cancer

Zhang, Jiayu; Wang, Xue; Liu, Tongjun; Liu, Shi; Jing, Xiabin

doi:10.3109/10717544.2014.916768

Cited by 76 publications

(37 citation statements)

References 23 publications

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“…The cytotoxicity of T-PNPs, T-SLNs, and T-NLCs was tested in U87 MG cells using the MTT assay (Zhang et al, 2016). Briefly, cells were seeded in a 96-well plate at a density of 1 Â 10 4 cells/well and allowed to adhere for 24 h prior to the assay.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy?

Zhang

Chen

et al. 2016

Drug Delivery

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Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy?

Zhang

Chen

et al. 2016

Drug Delivery

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“…The cytotoxicity of 5-FU (1 µg/ml) and oxaliplatin (l-OHP) (2 µg/ml) (both Shanghai Lichen Biotechnology Co., Ltd.) were determined using the MTT assay-based Cell Growth Determination kit (Sigma-Aldrich; Merck Millipore), as described previously (12). Briefly, cells were cultured in a flat 96-well plate at a density of 1,000-10,000 cells/well until ~80% confluence was reached.…”

Section: Cellmentioning

confidence: 99%

The role of MRP1 in the multidrug resistance of colorectal cancer

Cao

Qin

et al. 2017

Oncology Letters

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Abstract. The role of multidrug resistance associated protein 1 (MRP1) in the multidrug resistance (MDR) of colorectal cancer (CRC) remains unclear. The present study aimed to investigate the effect of MRP1 in MDR CRC and its therapeutic potential for the treatment of patients with this disease. The human MDR CRC cell lines HCT-8 and Colo205 were established through stable exposure to 5-florouracil (5-FU) over a 5-month period. MRP1 was knocked-down in MDR CRC cells through the transfection of short hairpin RNA targeting MRP1 (shMRP1). Western blotting was performed to assess the efficiency of this silencing. MTT and apoptosis assays were conducted to detect cell viability and apoptosis, respectively. ; all P<0.001). These results demonstrate that MRP1 serves a role in the MDR phenotype of CRC through inhibiting apoptosis and may serve as a potential therapeutic target for inhibition, which would increase the efficacy of other chemotherapeutic agents in the treatment of CRC.

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“…Electrospinning is a technique mostly used for producing polymeric nano-or micro-fibers. The advantage of this method is that structure and morphology of the fiber can be controlled, by modifying several parameters such as polymer type, concentration of solution, solvent or electrospinning conditions (Zhang et al, 2011;Zhang et al, 2016). Two nozzle electrospinning technique was used in this study to provide simultaneous spinning of fiber polymer and CsAloaded nanoparticles to obtain nanoparticle-loaded fibers (Pu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

In vivo tissue distribution and efficacy studies for cyclosporin A loaded nano-decorated subconjunctival implants

et al. 2016

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Biodegradable implants are promising drug delivery systems for sustained release ocular drug delivery with the benefits such as minimum systemic side effects, constant drug concentration at the target site and getting cleared without surgical removal. Dry eye syndrome (DES) is a common disease characterized with the changes in ocular epithelia surface and results in inflammatory reaction that might lead to blindness. Cyclosporin A (CsA) is a cyclic peptide that is frequently employed for the treatment of DES and it needs to be applied several times a day in tear drops form. The aim of this study was to evaluate in vivo behavior and efficacy of the developed nano-decorated subconjunctival implant systems for sustained release CsA delivery. Biodegradable Poly-ɛ-caprolactone (PCL) implant or micro-fiber implants containing CsA loaded poly-lactide-co-glycolide (85:15) (PLGA) or PCL nanoparticles were prepared in order to achieve sustained release. Two of the formulations PCL-PLGA-NP-F and PCL-PCL-NP-I were selected for in vivo evaluation based on their in vitro characteristics determined in our previous study. In this study, formulations were implanted to Swiss Albino mice with induced dry eye syndrome to investigate the ocular distribution of CsA following subconjunctival implantation and to evaluate the efficacy. Tissue distribution study indicated that CsA was present in ocular tissues such as cornea, sclera and lens even 90 days after the application and blood CsA levels were found lower than ocular tissues. Efficacy studies also showed that application of CsA-loaded fiber implant formulation resulted in faster recovery based on their staining scores.

show abstract

Antitumor activity of electrospun polylactide nanofibers loaded with 5-fluorouracil and oxaliplatin against colorectal cancer

Cited by 76 publications

References 23 publications

Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy?

Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy?

The role of MRP1 in the multidrug resistance of colorectal cancer

In vivo tissue distribution and efficacy studies for cyclosporin A loaded nano-decorated subconjunctival implants

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