Enantioselective gas chromatographic assay with electron-capture detection for amlodipine in biological samples

A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the estimation of amlodipine in human plasma

“…Several analytical methods have been reported in the literature for monitoring plasma levels of amlodipine. The techniques used in these methods include HPLC with fluorimetric detection (Tatar and Atmaca, 2001), HPLC with ultraviolet detection (Luska et al, 1997), HPLC with electrochemical detection (Shimooka et al, 1989;Josefsson et al, 1995), high-performance thinlayer chromatography-densitometry (Pandya et al, 1995) and gas chromatography (GC) with electron capture detection (Scharpf et al, 1994;Monkman et al, 1996). The reported HPLC methods require laborious extraction procedures like liquid-liquid extraction or solid-phase extraction involving drying and reconstitution, long run-time, high quantification limit, low recovery and large plasma volume.…”

Spectrophotometric determination of some calcium channel blockers using Sulfochlorophenol-S, Bromopyrogallol red, Eriochromecyanine-R and Pyrocatechoil violet

“…Calcium channel blockers have been determined by a variety of analytical techniques, such as HPLC [2][3][4][5][6][7][8][9][10], HPTLC [11][12][13][14][15][16], gas chromatography [17][18][19][20][21], Micellar electrokinetic chromatography [22][23][24][25], electroanalytical methods [26][27][28] and spectrophotometric methods [29][30][31][32][33][34].…”

Simple and rapid HPLC method for determination of amlodipine in human serum with fluorescence detection and its use in pharmacokinetic studies

“…The sensitivity of published HPLC-UV methods due to low absorbance of the drug is inadequate for pharmacokinetic studies and therapeutic drug monitoring [1,2]. In described gas chromatographic (GC) methods involving capillary column and electron capture detection although the sensitivity is improved, however, thermal decomposition of the drug under GC condition is the major problem [3][4][5]. Although determination of amlodipine in nanogram level was achieved by published HPLC-EC methods [6][7][8], however, they limited by low recovery [6], long retention time of the drug and internal standard (I.S.)…”