Simple and rapid HPLC method for determination of amlodipine in human serum with fluorescence detection and its use in pharmacokinetic studies

Bahrami, Gh.; Mirzaeei, S H

doi:10.1016/j.jpba.2004.05.016

Cited by 78 publications

(47 citation statements)

References 14 publications

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“…Plasma amlodipine concentrations were analysed using validated high-performance liquid chromatography with fluorescence detection as described previously with a slight modification [17]. A linearity calibration curve in the range of 0.1-10 ng ml − 1 was established for amlodipine ( r 2 = 0.9997).…”

Section: Drug Analysis and Pharmacokinetic Analysismentioning

confidence: 99%

Effect of ABCB1 (MDR1) haplotypes derived from G2677T/C3435T on the pharmacokinetics of amlodipine in healthy subjects

Kim

Park

2006

Brit J Clinical Pharma

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AimWe aimed to investigate the effect of the ABCB1 gene on the pharmacokinetics of amlodipine. MethodsBased on polymorphisms of the ABCB1 gene at positions 2677 and 3435, 26 healthy male participants were divided into three groups: subjects with 2677GG/3435CC ( n = 9), 2677GT/3435CT ( n = 9) and 2677TT/3435TT ( n = 8). After a single-dose administration of 5 mg amlodipine, plasma concentrations of amlodipine were measured and its pharmacokinetic characteristics were compared according to ABCB1 genotype.

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Section: Drug Analysis and Pharmacokinetic Analysismentioning

confidence: 99%

Effect of ABCB1 (MDR1) haplotypes derived from G2677T/C3435T on the pharmacokinetics of amlodipine in healthy subjects

Kim

Park

2006

Brit J Clinical Pharma

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“…Author concluded that this method is simple, fast, with higher resolution. The one step extraction procedure makes this method preferable compared to multiple extraction and washing steps in other previously published method [14]. Another study of spectrophotometric method for the determination of amlodipine besylate (ADB) in pure form and in pharmaceutical preparations was developed by Alizadeh and Hemati (2014).…”

Section: 4mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

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“…Several analytical methods for quantifying dofetilide (LCMS [24], HPLC [21,25] and RIA [26] or amlodipine (HPLC [9,[27][28][29][30][31], HPLC with radioactivity detection [32], HPLC with electrochemical detection [33], LCMS [34][35][36], GCMS [37] and GC with electron capture detection [38] in biological fluids have been reported. Unfortunately, all of these described methods do not allow the quantification of both drugs in the same run.…”

Section: Amlodipine Besylate (Norvascmentioning

confidence: 99%

Simultaneous Determination of Dofetilide and Amlodipine in Plasma by HPLC

Kaddar¹,

Pilote²,

Wong³

et al. 2013

J Chromat Separation Techniq

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Dofetilide is a Class III antiarrhythmic drug useful in the management of atrial fibrillation but also known to prolong the QT interval on the ECG and to induce malignant ventricular tachyarrhythmias such as torsades de pointes (TDP). Drugs with calcium channel-blocking properties contribute to decrease the incidence of TDP. Amlodipine is a dihydropyridine calcium channel antagonist. Considering the potentially safe and useful combined use of dofetilide and amlodipine, we undertook drug biotransformation studies. To perform studies aiming to evaluate the possible pharmacokinetic interaction between these drugs, a unique sensitive HPLC assay able to quantify both drugs simultaneously was required. A sensitive and specific HPLC method using a tandem of UV/fluorescence detection was described for the analysis of amlodipine and dofetilide in plasma. The within-day and between-day precision studies showed good reproducibility with coefficients of variation less than 10% for all the analytes. The limits of detection were 0.5 ng/mL and 0.25 ng/mL and the limit of quantification were 1.7 ng/mL and 0.8 ng/mL for dofetilide and amlodipine respectively. This new method could be of great value in many applications such as in vitro and in vivo pharmacokinetic and drug-drug interactions studies, as well as therapeutic drug monitoring.

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Simple and rapid HPLC method for determination of amlodipine in human serum with fluorescence detection and its use in pharmacokinetic studies

Cited by 78 publications

References 14 publications

Effect of ABCB1 (MDR1) haplotypes derived from G2677T/C3435T on the pharmacokinetics of amlodipine in healthy subjects

Effect of ABCB1 (MDR1) haplotypes derived from G2677T/C3435T on the pharmacokinetics of amlodipine in healthy subjects

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Simultaneous Determination of Dofetilide and Amlodipine in Plasma by HPLC

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