A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the estimation of amlodipine in human plasma

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of amlodipine in human plasma. Amlodipine was extracted from human plasma by using a solid-phase extraction technique. Imipramine was used as the internal standard. A Hypersil BDS C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at sub-nanogram levels. The proposed method has been validated for a linear range of 0.1-10.0 ng/mL with correlation coefficient >or=0.9990. The intrarun and interrun precision and accuracy were within 10.0%. The overall recovery for amlodipine was 63.67%. Total run time was 3.2 min only.

Current Analytical Methods For Amlodipine And Its Formulations: A Review

“…the recovery and stability studies were done using this method. The authors concluded that this method is rapid sensitive accurate and simple, therefore it can be used in determination of amlodipine in human plasma for pharmacokinetic and bioequivalence studies [35]. A new LC-MS-MS method for the simultaneous determination of amlodipine, losartan and losartic acid in human plasma was developed and validated by Karra and co-workers.…”

Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

“…Amlodipine besylate is official in IP [2] and BP [3] and Hydrochlorthiazide is official in IP [2], BP [3] and USP [4]. Literature survey revealed that various methods such as UV [9,10,15,16,19,20], HPLC [11,17], HPTLC [12,18], LC-MS/MS [13] and UPLC [14] are available in single and combination with other drugs. However, no spectrophotometric method has yet been reported for simultaneous estimation of amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide in tablet dosage forms.…”

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“…Capillary electrophoresis methods were also used for the analysis of AML in its formulations and biological fluids [15,16]. AML was also determined using liquid chromatography mass spectrometry (LC-MS) [17,18]. 1, 2-naphthoquinone-4-sulphonate (NQS) has been used as a chromogenic reagent for the colorimetric determination of many pharmaceutical amines [19][20][21].…”