The identification and separation of chiral pesticides R/S-profenofos are of great significance due to different biological toxicity of chiral isomers. In the study, we designed two novel ligands: (2,2 0 :6 0 ,2 00 -terpyridin)-6-yl(5-methoxy-1H-pyrazol-1-yl) methanethione (TMMT) and (2,2 0 :6 0 ,2 00 -terpyridin)-6-yl(5-methoxy-1H-pyrazol-1-yl) methanone (TMMO), and taking them as ligands coordinated with uranyl, two novel complexes [Uranyl-TMMT] 2+ and [Uranyl-TMMO] 2+ as receptors were successfully constructed. The complexation and enantioselectivity of two receptors towards R/S-profenofos as guests