Employing different types of phytoestrogens improve bone mineralization in bisphenol A stimulated osteoblast

Thent, Zar Chi; Froemming, Gabriele Ruth Anisah; Ismail, Aletza Binti Mohd; Fuad, Syed Baharom Syed Ahmad; Muid, Suhaila Abd

doi:10.1016/j.lfs.2018.08.057

Cited by 6 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given the key role of osteoblasts in bone physiology, these results suggest that BPA exerts adverse effects on bone, in line with reports of the negative impact of this endocrine disruptor on many other human tissues [16][17][18]. These findings are in line with previous observations in mouse [14] and human fetal [19] osteoblast cell lines. This is the first study to examine these effects in human osteoblasts obtained by primary culture.…”

Section: Discussionsupporting

confidence: 91%

Repercussions of Bisphenol A on the Physiology of Human Osteoblasts

García-Recio

Costela‐Ruiz

Melguizo‐Rodríguez

et al. 2022

IJMS

View full text Add to dashboard Cite

(1) Background: Bisphenol A (BPA) is an endocrine disruptor that is widely present in the environment and exerts adverse effects on various body tissues. The objective of this study was to determine its repercussions on bone tissue by examining its impact on selected functional parameters of human osteoblasts. (2) Methods: Three human osteoblast lines were treated with BPA at doses of 10−5, 10−6, or 10−7 M. At 24 h post-treatment, a dose-dependent inhibition of cell growth, alkaline phosphatase activity, and mineralization was observed. (4) Results: The expression of CD54 and CD80 antigens was increased at doses of 10−5 and 10−6 M, while the phagocytic capacity and the expression of osteogenic genes (ALP, COL-1, OSC, RUNX2, OSX, BMP-2, and BMP-7) were significantly and dose-dependently reduced in the presence of BPA. (5) Conclusions: According to these findings, BPA exerts adverse effects on osteoblasts by altering their differentiation/maturation and their proliferative and functional capacity, potentially affecting bone health. Given the widespread exposure to this contaminant, further human studies are warranted to determine the long-term risk to bone health posed by BPA.

show abstract

Section: Discussionsupporting

confidence: 91%

Repercussions of Bisphenol A on the Physiology of Human Osteoblasts

García-Recio

Costela‐Ruiz

Melguizo‐Rodríguez

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…After exposure to genistein, expression of the ocn gene was triggered, and cell mineralization took place in MC3T3-E1 cells. In human osteoblast-like hFOB 1.19 cells, genistein treatment reverted bisphenol A-induced demineralization of bone cells by elevating cellular OCN and osteonectin levels . Interestingly, knocking-down ERα concurrently inhibited genistein-induced Runx2 and OCN mRNA expressions.…”

Section: Discussionmentioning

confidence: 95%

“…In human osteoblast-like hFOB 1.19 cells, genistein treatment reverted bisphenol A-induced demineralization of bone cells by elevating cellular OCN and osteonectin levels. 50 Interestingly, knocking-down ERα concurrently inhibited genistein-induced Runx2 and OCN mRNA expressions. Consequently, genistein is beneficial for osteogenesis by stimulating osteogenesis-associated gene expressions through an ERα-dependent pathway.…”

Section: ■ Discussionmentioning

confidence: 97%

Genistein Improves Bone Healing via Triggering Estrogen Receptor Alpha-Mediated Expressions of Osteogenesis-Associated Genes and Consequent Maturation of Osteoblasts

Tai

Cherng

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

Osteoporosis-associated fractures may cause higher morbidity and mortality. Our previous study showed the effects of genistein, a phytoestrogen, on the induction of estrogen receptor alpha (ERα) gene expression and stimulation of osteoblast mineralization. In this study, rat calvarial osteoblasts and an animal bone defect model were used to investigate the effects of genistein on bone healing. Treatment with genistein caused a time-dependent increase in alkaline phosphatase (ALP) activity in rat osteoblasts. Levels of cytosolic and nuclear ERα significantly augmented following exposure to genistein. Subsequently, genistein elevated levels of ALP mRNA and protein in rat osteoblasts. Moreover, genistein induced other osteogenesis-associated osteocalcin and Runx2 mRNA and protein expressions. Knocking-down ERα using RNA interference concurrently inhibited genistein-induced Runx2, osteocalcin, and ALP mRNA expression. Attractively, administration of ICR mice suffering bone defects with genistein caused significant increases in the callus width, chondrocyte proliferation, and ALP synthesis. Results of microcomputed tomography revealed that administration of genistein increased trabecular bone numbers and improved the bone thickness and volume. This study showed that genistein can improve bone healing via triggering ERα-mediated osteogenesis-associated gene expressions and subsequent osteoblast maturation.

show abstract

“…BPA has a harmful effect on bone tissue since it can interact via estrogenic receptors with the two main bone tissue populations, osteoblasts and osteoclasts, possibly compromising bone health [1]. BPA inhibits the growth of murine (MC3T3-E1 cell line) [9] and human fetal (hFOB 1.19 cell line) osteoblasts [10] and human osteoblasts obtained by primary culture from bone implants [11]. This growth inhibition results from apoptosis induction, which compromises cell viability in a dose-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

“…This growth inhibition results from apoptosis induction, which compromises cell viability in a dose-dependent manner. BPA can also affect the function of this cell population by inhibiting alkaline phosphatase (ALP) synthesis and therefore the mineralization process [10][11][12]. BPA treatment has been found to alter the gene expression of RUNX2, osterix (OSX), β-catenin, collagen-1 (COL-1), osteocalcin (OSC), and bone morphogenetic proteins (BMPs) 2 and 7 (BMP-2 and BPM-7) [9][10][11]13], and the expression of these osteogenic markers is associated with the differentiation and maturation and, therefore, functional capacity of osteoblasts [14].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Osteogenic Gene Expression by Human Osteoblasts Cultured in the Presence of Bisphenols BPF, BPS, or BPAF

García-Recio

Costela‐Ruiz

Illescas‐Montes

et al. 2023

IJMS

View full text Add to dashboard Cite

Bone effects attributed to bisphenols (BPs) include the inhibition of growth and differentiation. This study analyzes the effect of BPA analogs (BPS, BPF, and BPAF) on the gene expression of the osteogenic markers RUNX2, osterix (OSX), bone morphogenetic protein-2 (BMP-2), BMP-7, alkaline phosphatase (ALP), collagen-1 (COL-1), and osteocalcin (OSC). Human osteoblasts were obtained by primary culture from bone chips harvested during routine dental work in healthy volunteers and were treated with BPF, BPS, or BPAF for 24 h at doses of 10−5, 10−6, and 10−7 M. Untreated cells were used as controls. Real-time PCR was used to determine the expression of the osteogenic marker genes RUNX2, OSX, BMP-2, BMP-7, ALP, COL-1, and OSC. The expression of all studied markers was inhibited in the presence of each analog; some markers (COL-1; OSC, BMP2) were inhibited at all three doses and others only at the highest doses (10−5 and 10−6 M). Results obtained for the gene expression of osteogenic markers reveal an adverse effect of BPA analogs (BPF, BPS, and BPAF) on the physiology of human osteoblasts. The impact on ALP, COL-1, and OSC synthesis and therefore on bone matrix formation and mineralization is similar to that observed after exposure to BPA. Further research is warranted to determine the possible contribution of BP exposure to the development of bone diseases such as osteoporosis.

show abstract

Employing different types of phytoestrogens improve bone mineralization in bisphenol A stimulated osteoblast

Cited by 6 publications

References 32 publications

Repercussions of Bisphenol A on the Physiology of Human Osteoblasts

Repercussions of Bisphenol A on the Physiology of Human Osteoblasts

Genistein Improves Bone Healing via Triggering Estrogen Receptor Alpha-Mediated Expressions of Osteogenesis-Associated Genes and Consequent Maturation of Osteoblasts

Modulation of Osteogenic Gene Expression by Human Osteoblasts Cultured in the Presence of Bisphenols BPF, BPS, or BPAF

Contact Info

Product

Resources

About