Modulation of Osteogenic Gene Expression by Human Osteoblasts Cultured in the Presence of Bisphenols BPF, BPS, or BPAF

García-Recio, Enrique; Costela‐Ruiz, Víctor J.; Illescas‐Montes, Rebeca; Melguizo‐Rodríguez, Lucia; García-Martínez, Olga; Ruíz, Concepción; Luna-Bertos, Elvira De

doi:10.3390/ijms24054256

Cited by 6 publications

(4 citation statements)

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“…On the other hand, the observed effect of BPA analogues on osteoblasts proliferation and differentiation and/or maturation is closely related to the effect recently described by (García-Recio et al 2023 ), when analyzing the modulation of osteogenic markers in the presence of these BPs.…”

Section: Discussionsupporting

confidence: 78%

“…It also inhibited mineralization by reducing alkaline phosphatase (ALP) synthesis and the consequent formation of calcium nodules. At a molecular level, BPA and its analogs (BPF, BPS, and BPAF) inhibited the gene expression of osteogenic markers closely related to osteoblastogenesis and osteoblast function, i.e., RUNX2, OXS, OSC, ALP, COL-1, BMP-2, and BMP-7 (García-Recio et al 2022 , 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts

et al. 2023

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Bisphenol A (BPA) analogs, like BPA, could have adverse effects on human health including bone health. The aim was to determine the effect of BPF, BPS and BPAF on the growth and differentiation of cultured human osteoblasts. Osteoblasts primary culture from bone chips harvested during routine dental work and treated with BPF, BPS, or BPAF for 24 h at doses of 10–5, 10–6, and 10–7 M. Next, cell proliferation was studied, apoptosis induction, and alkaline phosphatase (ALP) activity. In addition, mineralization was evaluated at 7, 14, and 21 days of cell culture in an osteogenic medium supplemented with BP analog at the studied doses. BPS treatment inhibited proliferation in a dose-dependent manner at all three doses by inducing apoptosis; BPF exerted a significant inhibitory effect on cell proliferation at the highest dose alone by an increase of apoptosis; while BPAF had no effect on proliferation or cell viability. Cell differentiation was adversely affected by treatment with BPA analogs in a dose-dependent, observing a reduction in calcium nodule formation at 21 days. According to the results obtained, these BPA analogs could potentially pose a threat to bone health, depending on their concentration in the organism.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts

et al. 2023

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“…BPA and its analogs (BPF, BPS, and BPAF) adversely affect osteogenic gene expression in human osteoblasts. The analogs deregulated ALP, COL1A1, and OCN expression, inhibiting matrix formation and mineralization, similar to the effects of BPA [94]. BPA can alter bone formation by mimicking estrogen and competing for both ERα and ERβ.…”

Section: Mechanisms Of Endocrine Disruption and Bone Damagementioning

confidence: 75%

Endocrine Disruptor-Induced Bone Damage Due to Hormone Dysregulation: A Review

Iwobi,

Sparks

2023

IJMS

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Hormones are indispensable for bone development, growth, and maintenance. While many of the genes associated with osteogenesis are well established, it is the recent findings in endocrinology that are advancing the fields of bone biology and toxicology. Endocrine-disrupting chemicals (EDCs) are defined as chemicals that interfere with the function of the endocrine system. Here, we report recent discoveries describing key hormone pathways involved in osteogenesis and the EDCs that alter these pathways. EDCs can lead to bone morphological changes via altering hormone receptors, signaling pathways, and gene expression. The objective of this review is to highlight the recent discoveries of the harmful effects of environmental toxicants on bone formation and the pathways impacted. Understanding the mechanisms of how EDCs interfere with bone formation contributes to providing a comprehensive toxicological profile of a chemical.

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“…Therefore, folate supplementation can be suggested as a potential preventive against the adverse effects of environmental exposures, including BPA and its alternatives. However, further research to establish definitive conclusions is recommended, taking into account that other studies indeed showed potential adverse effects on bone development concerning BPA alternatives; in human osteoblast tissues exposed to different doses of BPF or BPS, the expression of multiple osteogenic markers were observed [79].…”

Section: Effects On Bone Developmentmentioning

confidence: 99%

Effects of Prenatal Exposure to Bisphenol A Substitutes, Bisphenol S and Bisphenol F, on Offspring’s Health: Evidence from Epidemiological and Experimental Studies

Algonaiman,

Almutairi,

Al Zhrani

et al. 2023

Biomolecules

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Pregnancy and lactation are critical periods for human well-being and are sensitive windows for pollutant exposure. Bisphenol A (BPA) is well demonstrated as a toxicant and has been replaced in the plastic industry with other bisphenol analogs that share similarities in structure and characteristics, most commonly Bisphenol S (BPS) and Bisphenol F (BPF). Maternal exposure to BPS or BPF can result in their accumulation in the fetal compartment, leading to chronic exposure and potentially limiting normal fetal growth and development. This review summarizes considerable findings of epidemiological or experimental studies reporting associations between BPS or BPF and impaired fetal growth and development. Briefly, the available findings indicate that exposure to the two bisphenol analogs during pregnancy and lactation can result in multiple disturbances in the offspring, including fetal growth restrictions, neurological dysfunctions, and metabolic disorders with the potential to persist throughout childhood. The occurrence of premature births may also be attributed to exposure to the two bisphenols. The possible mechanisms of actions by which the two bisphenols can induce such effects can be attributed to a complex of interactions between the physiological mechanisms, including impaired placental functioning and development, dysregulation of gene expression, altered hormonal balance, and disturbances in immune responses as well as induced inflammations and oxidative stress. In conclusion, the available evidence suggests that BPS and BPF have a toxic potential in a compartment level to BPA. Future research is needed to provide more intensive information; long-term studies and epidemiological research, including a wide scale of populations with different settings, are recommended. Public awareness regarding the safety of BPA-free products should also be enhanced, with particular emphasis on educating individuals responsible for the well-being of children.

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Modulation of Osteogenic Gene Expression by Human Osteoblasts Cultured in the Presence of Bisphenols BPF, BPS, or BPAF

Cited by 6 publications

References 50 publications

Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts

Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts

Endocrine Disruptor-Induced Bone Damage Due to Hormone Dysregulation: A Review

Effects of Prenatal Exposure to Bisphenol A Substitutes, Bisphenol S and Bisphenol F, on Offspring’s Health: Evidence from Epidemiological and Experimental Studies

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