Emissions from indoor dust inhibit proliferation of A549 cells and TNFα release from stimulated PBMCs

Differential Response of Mono Mac 6, BEAS-2B, and Jurkat Cells to Indoor Dust

“…Genes grouped under the GO category “cell proliferation” preferentially transmitted negative regulation of cell proliferation ( p = 0.04). Similarly, emissions of indoor dust and ambient particulates reduced epithelial cell proliferation in two recent studies (Baulig et al 2003; Mathiesen et al 2004). Consistently, genes promoting the progression through the cell cycle were significantly down-regulated in MM6 cells.…”

Building-related symptoms and inflammatory potency of dust from office buildings

“…Microorganisms and components of microbial origin in the dust may be a sign of microbial growth in the building and therefore also microbial exposure of the occupants. A lower secretion of IL‐8 and TNF‐ α from A549 cells after heating of office dust was partly explained by a reduction in the concentration of endotoxin in the dust (Mathiesen et al., 2004a), and emissions from heating of indoor dust were found to have an inhibitory effect on cell proliferation and mitochondrial activity in A549 cells (Mathiesen et al., 2004b). This was also shown for cigarette smoke (Lannan et al., 1994).…”

“…Previous studies have shown that sedimented dust from the indoor environment and several biological and chemical constituents such as endotoxins, glucans, allergens, surfactants and phthalates were able to trigger a response of proinflammatory cytokines such as IL‐8, IL‐6, and TNF‐ α from the A549 lung epithelial cell line (Allermann, 2001; Allermann et al., 2003; Hansen et al., 1997, 1999; Jepsen et al., 2004; Mathiesen et al., 2004a; Saraf et al., 1999). Furthermore, emissions of heated office dust have been shown to inhibit proliferation and mitochondrial activity in A549 cells (Mathiesen et al., 2004b). Dust from swine confinement buildings, waste sorting plants, cotton and composting of organic waste have also been shown to induce IL‐8 secretion from the A549 lung epithelial cell line (Allermann and Poulsen, 2000; Hansen et al., 1997; Palmberg et al., 1998).…”

Heating of indoor dust causes reduction in its ability to stimulate release of IL-8 and TNFalpha in vitro compared to non-heated dust

“…Suppressed TNF α production by noxious compounds may increase the health risk to the host caused by other foreign insults (Gardner, 1984). We have shown that the emissions from heated indoor dust cause suppression of LPS‐induced TNF α from PBMCs (Mathiesen et al., 2003a). In this context, decomposition compounds associated to resuspended residual particles and decomposition compounds and desorbed chemicals emitted during heating may pose an environmental air problem.…”

“…During heating of dust samples in our laboratory hot‐surface model, three fractions are generated: (i) the remains of the heated dust sample, denoted residuals, (ii) the emissions volatile at room temperature, and (iii) the condensate, condensed vapors of emissions. We developed in vitro methods for testing the biologic effect of emissions from heated dust at temperatures realistic for indoor equipment (Mathiesen et al., 2003a,b). These emissions proved to have a toxic effect in the cell cultures applied.…”