Differential Response of Mono Mac 6, BEAS-2B, and Jurkat Cells to Indoor Dust

Riechelmann, Herbert; Deutschle, Tom; Grabow, Ariane; Heinzow, Birger; Butte, W.; Reiter, Rudolf

doi:10.1289/ehp.9874

Cited by 35 publications

(17 citation statements)

References 40 publications

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“…However, we observed no IL-6 and GMCSF release from BEAS2B cells cultured in DMEM with FBS in response to crude HDM ( Figure 6C). Although there have been previous reports that demonstrate the expression of proinflammatory cytokines, including IL-6 and GMCSF in BEAS2B cells in response to direct HDM treatment, they all used recombinant or purified HDMassociated allergens Der p1, Der p2, and Der p9 (10) as inducers and not the crude mix (48). Der p2, for instance, is shown to reconstitute TLR signaling in the absence of MD-2 (46).…”

Section: Discussionmentioning

confidence: 99%

House Dust Mite Allergens and the Induction of Monocyte Interleukin 1β Production That Triggers an IκBζ-Dependent Granulocyte Macrophage Colony-Stimulating Factor Release from Human Lung Epithelial Cells

Sundaram

Mitra

Gavrilin

et al. 2015

Am J Respir Cell Mol Biol

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Asthma is a chronic lung disease characterized by inflammation centered upon bronchial epithelium. House dust mite is one of the most common respiratory allergens that trigger exacerbations of asthma. IkBz (gene NFKBIZ) is a recently recognized member of the NF-kB family that can be induced in mononuclear phagocytes and lung epithelial cells and has been shown to play a prominent role in epithelial cell function. We therefore analyzed the role of IkBz in regulating lung epithelial cell cytokine responses to house dust mite mix (HDM). We found that human bronchial epithelial cells express IkBz and release IL-6 and granulocyte macrophage colony-stimulating factor (GMCSF) when cocultured with human monocytes and HDM. This response is blocked in the presence of IL-1 receptor antagonist (IL-1Ra), indicating that it is IL-1 mediated. Neither HDM-stimulated macrophages nor dendritic cells release IL-1b and subsequently induce cytokine release from the bronchial epithelial cells. Rhodobacter sphaeroides LPS (RS-LPS), a TLR4 antagonist, blocks the ability of HDM to induce IkBz and release GMCSF from epithelial cells cocultured with monocytes. Additionally, human bronchial epithelial cells show no induction of IkBz or cytokine responses to direct HDM stimulation. Finally, NFKBIZ small interfering RNA-mediated knockdown in the bronchial epithelial cells suppresses the release of IL-1-induced IL-6 and GMCSF. Our findings indicate a possible role for monocyte recruitment and lung epithelial cell IkBz in mediating asthma associated inflammation. Thus, IkBz, IL-1Ra, and RS-LPS deserve future study as potential modulators of house dust mite-induced asthma.

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Section: Discussionmentioning

confidence: 99%

House Dust Mite Allergens and the Induction of Monocyte Interleukin 1β Production That Triggers an IκBζ-Dependent Granulocyte Macrophage Colony-Stimulating Factor Release from Human Lung Epithelial Cells

Sundaram

Mitra

Gavrilin

et al. 2015

Am J Respir Cell Mol Biol

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“…On the other hand, similar dominant PAHs such as Nap and Phe were both found in outdoor and indoor dust, possibly due to the higher vapor pressure of Nap and Phe compared to B(b+k)F and subsequently transporting into indoor environment with outdoor air particles. The distribution difference of PAHs between outdoor and indoor dust should depend on the different anthropogenic source of PAHs contamination (Pengchai et al 2005;Riechelmann et al 2007). …”

Section: Pahs Levels In Indoor and Outdoor Dustmentioning

confidence: 99%

“…There are many emitting sources for PAHs in indoor environment including the smoking, cooking, and domestic fuel combustion (He et al 2005;Riechelmann et al 2007). PAHs in outdoor environment can be derived from the vehicle emission, power generation by coal combustion, petroleum refining, and waste burning (Peng et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cancer risk assessment of human exposure to polycyclic aromatic hydrocarbons (PAHs) via indoor and outdoor dust based on probit model

Kang

Shao

et al. 2014

Environ Sci Pollut Res

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In the present study, the polycyclic aromatic hydrocarbons (PAHs) in indoor dust and outdoor dust including road and window dust around the traffic road in Hunan Province, China, were sampled and detected. The ∑PAHs in indoor dust ranged from 5007-24,236 ng g(-1), with a median of 14,049 ng g(-1). The ∑PAHs in road dust ranged from 3644-12,875 ng g(-1), with a median of 10,559 ng g(-1). The ∑PAHs in window dust ranged from 803-12,590 ng g(-1), with a median of 5459 ng g(-1). Similar pattern of PAHs was observed in road and window dust except in H3W and H4W samples, which was dominated by naphthalene (Nap), benzo(b+k)fluoranthene (B(b+k)F), phenanthrene (Phe), and fluorine (Fle). Indoor dust showed slightly different PAHs profiles, which was dominated by Nap, fluoranthene (Fla) and Phe. Risk assessment indicated that dermal contact and dust ingestion exposure pathways were more important than the inhalation pathway. Cancer risk of PAHs via dust varied from 2.73 × 10(-8)-8.04 × 10(-6), with a median of 2.06 × 10(-6) for children, and from 2 × 10(-8)-5.89 × 10(-6), with a median of 1.52 × 10(-6) for adult. Probit model showed that 76 and 71 % of samples in the sampling area would result in the risk of children and adult exposure to PAHs via dust higher than the acceptable level (1 × 10(-6)), respectively.

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“…In addition, PAH sources differ between indoor and outdoor dust. Previous studies have suggested that there are additional sources of PAHs in indoor dust, such as domestic heating, smoking, and cooking, in addition to those of outdoor dust, such as heavy motor vehicle traffic release and industrial emissions (Riechelmann et al 2007;Maertens et al 2008;Wang et al 2013). Therefore, it is necessary to evaluate the concentration and identify the source of PAHs in indoor dust to assess the risk to human health.…”

Section: Introductionmentioning

confidence: 99%

Assessment of polycyclic aromatic hydrocarbons in indoor dust from varying categories of rooms in Changchun city, northeast China

Wang

Nie

et al. 2016

Environ Geochem Health

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Sixteen polycyclic aromatic hydrocarbons (PAHs) were isolated from indoor dust from various categories of rooms in Changchun city, northeast China, including dormitory, office, kitchen, and living rooms. PAH concentrations ranged from 33.9 to 196.4 μg g and 21.8 to 329.6 μg g during summer and winter, respectively, indicating that total PAH concentrations in indoor dust are much higher than those in other media from the urban environment, including soils and sediments. The percentage of five- to six-ring PAHs was high, indicating that PAHs found in indoor dust mainly originate from pyrolysis rather than a petrogenic source. Rooms were divided into three groups using cluster analysis on the basis of 16 PAH compositions, namely smoke-free homes, homes exposed to smoke and offices. Results showed that the source of PAHs in smoke-free residential homes is primarily the burning of fossil fuels. In addition to the burning of fossil fuels, biomass combustion and cooking contributed to PAHs in houses exposed to smoke (including kitchens). Motor vehicles are an additional source of PAHs in offices because of greater interactions with the outdoor environment. The results of health risk assessment showed that the cancer risk levels by dermal contact and ingestion are 10- to 10-fold higher than that by inhalation, suggesting that ingestion and dermal contact of carcinogenic PAHs in dust are more important exposure routes than inhalation of PAHs from air. Although the results showed high potential of PAH concentrations in indoor dust in Changchun for human health risk, caution should be taken to evaluate the risk of PAHs calculated by USEPA standard models with default parameters because habitation styles are different in various categories of rooms.

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Differential Response of Mono Mac 6, BEAS-2B, and Jurkat Cells to Indoor Dust

Cited by 35 publications

References 40 publications

House Dust Mite Allergens and the Induction of Monocyte Interleukin 1β Production That Triggers an IκBζ-Dependent Granulocyte Macrophage Colony-Stimulating Factor Release from Human Lung Epithelial Cells

House Dust Mite Allergens and the Induction of Monocyte Interleukin 1β Production That Triggers an IκBζ-Dependent Granulocyte Macrophage Colony-Stimulating Factor Release from Human Lung Epithelial Cells

Cancer risk assessment of human exposure to polycyclic aromatic hydrocarbons (PAHs) via indoor and outdoor dust based on probit model

Assessment of polycyclic aromatic hydrocarbons in indoor dust from varying categories of rooms in Changchun city, northeast China

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