Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases (Adv. Mater. 30/2022)

Kabay, Gözde; DeCastro, Jonalyn; Altay, Alara; Smith, Kasey; Lu, Hsiang‐Wei; Capossela, Antonia McDonnell; Moarefian, Maryam; Aran, Kiana; Dincer, Can

doi:10.1002/adma.202270224

Cited by 6 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 29,31,37 ] The average response time of SARS‐CoV‐2 antigens is 38.9 s (Figure 4d), much shorter than the current gold‐standard assay, which requires a few hours. [ 4,11,21 ] For HRV detection, we collected nasopharyngeal swab specimens from 70 HRV patients (P1–P70), 26 BP patients (B1–B26), 30 HRV‐negative patients (N1–N30) and 21 healthy subjects (H1–H21). Figure 4e,f indicate that the diagnostic sensitivity reached 100% (70 of 70, CI 96.7–100) and the clinical specificity reached 100% (77 of 77, CI 98.9–100).…”

Section: Resultsmentioning

confidence: 99%

“…[ 1–3 ] Globally, Mtb has infected over 2.5 billion people, with ≈10 million new cases every year, leading to a global tuberculosis (TB) pandemic. [ 2–4 ] The key for TB screening is rapid biomarker‐based tests for precise diagnosis and early treatment. [ 1,4 ] The current gold standard diagnostic method is bacterial culture, a clinical approach that identifies as few as 10 colony‐forming units (CFU) mL −1 bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…[ 2–4 ] The key for TB screening is rapid biomarker‐based tests for precise diagnosis and early treatment. [ 1,4 ] The current gold standard diagnostic method is bacterial culture, a clinical approach that identifies as few as 10 colony‐forming units (CFU) mL −1 bacteria. [ 5,6 ] However, it usually takes 10–14 days even with a rapid liquid culture because mycobacterium is a slow‐growing bacterium and the number of bacterial units is not detectable at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…[ 6,12–14 ] Although these integrated and automated Xpert systems provide results within minutes, they exhibit diminished sensitivity (65–73%) in unprocessed samples from latent patients who have dormant and low‐abundance pathogens. [ 4,13–15 ] Thus, the WHO TB End Strategy requires high‐quality patient‐centered diagnostic tools that are sensitive, rapid, reliable, easy‐to‐use, and capable of detecting unprocessed biological samples. [ 1,15 ]…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Dai

Yang

Xiong

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

For epidemic prevention and control, molecular diagnostic techniques such as field-effect transistor (FET) biosensors is developed for rapid screening of infectious agents, including Mycobacterium tuberculosis, SARS-CoV-2, rhinovirus, and others. They obtain results within a few minutes but exhibit diminished sensitivity (<75%) in unprocessed biological samples due to insufficient recognition of low-abundance analytes. Here, an electro-enhanced strategy is developed for the precise detection of trace-level infectious agents by liquid-gate graphene field-effect transistors (LG-GFETs). The applied gate bias preconcentrates analytes electrostatically at the sensing interface, contributing to a 10-fold signal enhancement and a limit of detection down to 5 × 10 −16 g mL −1 MPT64 protein in serum. Of 402 participants, sensitivity in tuberculosis, COVID-19 and human rhinovirus assays reached 97.3% (181 of 186), and specificity is 98.6% (213 of 216) with a response time of <60 s. This study solves a long-standing dilemma that response speed and result accuracy of molecular diagnostics undergo trade-offs in unprocessed biological samples, holding unique promise in high-quality and population-wide screening of infectious diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Dai

Yang

Xiong

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…[ 41,42 ] Other main semi‐direct methods include detection of surface proteins of virus within body tissues such as blood or a biopsy sample extracted from lesions. [ 43 ]…”

Section: Biosensorsmentioning

confidence: 99%

Addressing the Silent Spread of Monkeypox Disease with Advanced Analytical Tools

Bhalla

Payam

2022

Small

View full text Add to dashboard Cite

show abstract

Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag

Costa,

Pimentel,

Poker

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low‐cost ultra‐dense electrochemical chips, the unprecedented single‐response multiplexing of typical label‐free biosensors is reported. Using a cheap, handheld one‐channel workstation and a single redox probe, i.e., ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi‐reference electrodes, Au (0.0 V) and Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding steps, representing an advantage over the existing non‐spatially resolved single‐response multiplex strategies. As proof of concept, peptide‐tethered immunosensors are demonstrated to provide the duplex detection of COVID‐19 antibodies from distinct samples, thereby doubling the throughput while achieving 100% accuracy in serum samples. We envision the approach to enable broad applications in high‐throughput and multi‐analyte platforms, as it can be tailored to other biosensing devices and formats.This article is protected by copyright. All rights reserved

show abstract

Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases (Adv. Mater. 30/2022)

Cited by 6 publications

References 0 publications

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Addressing the Silent Spread of Monkeypox Disease with Advanced Analytical Tools

Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag

Contact Info

Product

Resources

About