Emergence of Metallo- β- Lactamase producing Isolates of Pseudomonas aeruogenosa Urinary Tract Infection in Children

Abstract

Citation: Chateen I. Ali Pambuk, Sabah M. Salih and Fatma Mustafa Mohammed, Emergence of Metallo-b-Lactamase producing Isolates of Pseudomonas aeruogenosa Urinary Tract Infection in Children, J Pure Appl Microbiol., 2019; 13(4):2309-2316. https:// AbstractInfections with Pseudomonas aeruginosa isolates producing Metallo-b-Lactamase (MBL) is a serious worldwide infections. It is associated with, higher mortality rates, more severe disease implicated and with a higher incidence of invasive disease than infections with the bacterial isolate of MBLnonproducers. This bacteria disseminated in many countries worldwide, with no reports available about their presence in Iraq. The present study was conducted to investigate the changes and the intensity of antibiotics resistance pattern of pediatric urinary tract infection (UTI) in duration of 2 years as well as reporting the first emergence of Metallo-b-Lactamase producing P aerugenosa in Iraq. Standard methods used for isolation and identification of bacteria. Carbapenem-resistance P. aeruginosa UTI isolates were tested for the presence of metallo-b-lactamases productino. This is the first report of the presence of Metallo-b-Lactamase producing P aerugenosa in Iraq region. The results showed no differences in resistance pattern among Gram positive bacteria isolated. While, their was a dramatic increase of Escherichia coli resistance which showed high sensitivity rate to cefotaxim (72.4%), recording an increasing rate of resistance by 12% during 2 years. Klebsiellae pneumoniae resistance profile were increased dramatically during 2 years to trimethoprim and cefotaxim to reach 30 % and 11% respectively, when compaired with our previous study. Regarding P. aeroginosae, there was further increase in resistance toward azithromycin, gentamicin and oxacillin by recording 14%, 11.6% and 11.6% of resistance rates respectively. The striking results of the present study was to find the resistant bacterial isolates of P. aeruginosae toward imipineme. Precisely, 4 (31.8%) out of 13 UTI isolates was found to be resistant to imipineme. In conclusion their was an different increasing resistance rates of isolates toward antibiotics, also an emergence of new MBL producing P. eaurogenosa.