Embryos derived from vitrified oocytes are not at an increased risk of aneuploidy and demonstrate excellent implantation rates: a paired, randomized controlled trial (RCT) in an infertile population

Forman, Eric J.; Ferry, K.M.; Benson, M.R.; Campos, J.; Treff, Nathan R.; Scott, Richard T.

doi:10.1016/j.fertnstert.2011.07.167

Cited by 4 publications

(2 citation statements)

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“…32 An example of the power of paired analysis was recently described in the evaluation of cumulus cell gene expression markers of reproductive potential 37 and the evaluation of the impact of biopsy 33 and oocyte vitrification. 38 The distinct advantage of eliminating all patient-specific variables from the evaluation of putative embryo specific markers or interventions should become the standard for ongoing research in embryo selection.…”

Section: Beyond Comprehensive Chromosome Screeningmentioning

confidence: 99%

Comprehensive Chromosome Screening and Embryo Selection: Moving Toward Single Euploid Blastocyst Transfer

et al. 2012

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Interest in using aneuploidy screening to select embryos has been renewed with the introduction of new methods for comprehensive chromosome screening (CCS) that evaluate all 24 chromosomes. With a series of experiments providing level I evidence of accuracy, reliability, safety, predictive value, and clinical efficacy, CCS-based selection of a single euploid blastocyst may provide an opportunity to finally realize the potential benefits and practical application of elective single embryo transfer. Furthermore, by incorporating CCS into research and development to control for chromosomal contribution to reproductive potential, additional biomarkers may now be more readily identified that help to further enhance the efficacy of embryo selection technology. This review describes the critical components of a valid CCS embryo selection methodology and discusses the limitations and opportunities for future routine application in reproductive medicine.

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Section: Beyond Comprehensive Chromosome Screeningmentioning

confidence: 99%

Comprehensive Chromosome Screening and Embryo Selection: Moving Toward Single Euploid Blastocyst Transfer

et al. 2012

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“…This may represent an extremely powerful approach to finding and validating new markers of reproductive competence. In fact, there are now a number of preliminary studies involving the application of DNA fingerprinting to help determine the impact of embryo biopsy [3] or oocyte vitrification [4] on reproductive competence, and to characterize the predictive value of cumulus cell gene expression profiles [5], telomere DNA content [6], or mitochondrial DNA copy number [7] for reproductive potential.…”

Section: Introductionmentioning

confidence: 99%

Trophectoderm DNA fingerprinting by quantitative real-time PCR successfully distinguishes sibling human embryos

Scott

Tao

et al. 2014

J Assist Reprod Genet

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Purpose To validate a novel and more practical system for trophectoderm DNA fingerprinting which reliably distinguishes sibling embryos from each other. Methods In this prospective and blinded study two-cell and 5-cell samples from commercially available sibling cell lines and excess DNA from trophectoderm biopsies of sibling human blastocysts were evaluated for accurate assignment of relationship using qPCR-based allelic discrimination from 40 single nucleotide polymorphisms (SNPs) with low allele frequency variation and high heterozygosity. Results Cell samples with self relationships averaged 95.1± 5.9 % similarity. Sibling relationships averaged 57.2±5.9 % similarity for all 40 SNPs, and 40.8±8.2 % similarity for the 25 informative SNPs. Assignment of relationships was accomplished with 100 % accuracy for cell lines and embryos. Conclusions These data demonstrate the first trophectoderm qPCR-based DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. This methodology will empower research and development of new markers of, and interventions that influence embryonic reproductive potential.

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Vitrification versus slow freezing for women undergoing oocyte cryopreservation

Glujovsky¹,

Riestra

Sueldo

et al. 2014

Cochrane Database of Systematic Reviews

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Embryos derived from vitrified oocytes are not at an increased risk of aneuploidy and demonstrate excellent implantation rates: a paired, randomized controlled trial (RCT) in an infertile population

Cited by 4 publications

References 0 publications

Comprehensive Chromosome Screening and Embryo Selection: Moving Toward Single Euploid Blastocyst Transfer

Comprehensive Chromosome Screening and Embryo Selection: Moving Toward Single Euploid Blastocyst Transfer

Trophectoderm DNA fingerprinting by quantitative real-time PCR successfully distinguishes sibling human embryos

Vitrification versus slow freezing for women undergoing oocyte cryopreservation

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