Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)

Islam, Nazrul; Islam, Tajul; Hossain, Munir; Bhattacharjee, Bakul; Hossain, Moazzem; Islam, SM Shahinul

doi:10.3329/ptcb.v27i1.35011

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…The current status is that soybean biolistic transformation still relies on an embryogenic callus, since it is the prerequisite for establishing a robust transformation system for a specific genotype. Selection for the amenability of an embryogenic callus induced from local elite varieties (genotypes) is the main focus (Joyner et al, 2010;Abbasi et al, 2016;Islam et al, 2017;Raza et al, 2020). An improved biolistic soybean transformation protocol was published using an embryogenic callus induced from an immature cotyledon explant (Finer, 2016), which is a robust one and can produce quite a lot of transgenic plants within 6-9 months.…”

Section: Biolistic Transformationmentioning

confidence: 99%

Progress in Soybean Genetic Transformation Over the Last Decade

Guo

Qiu

et al. 2022

Front. Plant Sci.

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Soybean is one of the important food, feed, and biofuel crops in the world. Soybean genome modification by genetic transformation has been carried out for trait improvement for more than 4 decades. However, compared to other major crops such as rice, soybean is still recalcitrant to genetic transformation, and transgenic soybean production has been hampered by limitations such as low transformation efficiency and genotype specificity, and prolonged and tedious protocols. The primary goal in soybean transformation over the last decade is to achieve high efficiency and genotype flexibility. Soybean transformation has been improved by modifying tissue culture conditions such as selection of explant types, adjustment of culture medium components and choice of selection reagents, as well as better understanding the transformation mechanisms of specific approaches such as Agrobacterium infection. Transgenesis-based breeding of soybean varieties with new traits is now possible by development of improved protocols. In this review, we summarize the developments in soybean genetic transformation to date, especially focusing on the progress made using Agrobacterium-mediated methods and biolistic methods over the past decade. We also discuss current challenges and future directions.

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Section: Biolistic Transformationmentioning

confidence: 99%

Progress in Soybean Genetic Transformation Over the Last Decade

Guo

Qiu

et al. 2022

Front. Plant Sci.

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“…These findings were in agreement with those reported by Abd El-Kadder & Hammad (2012) on D. indica L. They showed that the maximum shoot multiplication rate (7.1 shoots per explant) was obtained at 2.0mg L −1 of BAP. Islam et al (2017) reported that the efficiency of shoot bud formation was relied upon and enhanced by supplementing the medium with BA. Similarly, Miri (2020) reported that the increase in BA concentration boosted the induction of axillary shoots of Zingiber officinale Rosc Furthermore, (Zhang et al, 2020) concluded that stem explant which grows on an MS medium containing 1.0mg L -1 BA without NAA giving high shoot multiplication to Malus sieversii.…”

Section: Shooting Regeneration Stagementioning

confidence: 99%

Antimicrobial and In-vitro Cytotoxic Efficacy of Betulinic Acid from the Callus, Regeneration, and Mother Plant of Dillenia indica L.

et al. 2021

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I N THIS STUDY, we established of an application protocol for the micropropagation of Dillenia indica with an estimate of betulinic acid from callus, leaf, and regeneration plant. Moreover, the antimicrobial and cytotoxic efficacy of the crude extract and pure compound was compared. The highest number of shoots (3.667 shoots), leaf number per jar (27 leaves) and shoot length (3.667cm) were recorded from the MS medium containing 2.0mg L -1 of benzyl amino purine (BAP). Moreover, different physical elicitors (ultraviolet irradiation, microwave radiation, and light quality) were used on the production and increased the concentration of betulinic acid in callus of D. indica. The data revealed that the highest betulinic acid content (341.775mg/ 100g fresh weight) was recorded in callus microwaved with 200 watts for 10s. Which was three times more than the betulinic acid content recorded in the mother plant. In addition, different crude extracts of D. indica were used to determine their biological activity against six microorganisms. The data showed that the highest diameter of inhibition zones (17.0 mm) was recorded in callus culture extractions incubated under blue light with Candida albicans. The cytotoxic efficacy of betulinic acid and extract of callus, mother plant leaf or in vitro regeneration plants of D. indica were investigated against the Vero cell line by MTT. The results revealed that The IC 50 values were 115.7 ± 9.17, 114.98 ± 5.2, 59.45 ± 3.06, and 72.76 ± 6.93μgmL -1 of betulinic acid, callus extract, leaf, and regeneration plant, respectively.

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“…Most callus (88.8%) was produced in soybean sprouts cotyledon hook explants, and callus development from shoot tips formed embryos (79.4%), Proportion of formed embryo originating from cotyledon hook callus (76.3%) were produced in MS medium with the addition of 2.5 mg.L -1 BAP + 1.0 mg.L -1 NAA. The longest shoots (5.32 cm) was produced on BS-6 media [14].…”

Section: Introductionmentioning

confidence: 99%

The Induction of Callus in Four Diverse Black Soybeans (Glycine soja Sieb. & Zucc) with Various Combinations of Cytokinins and 2,4-Dichlorophenoxyacetinic Acid

Romeida¹,

Pujiwati²,

Supanjani³

et al. 2021

Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 202

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The black soybean (Glycine soya Sieb. & Zucc) is a species of legume native of East Asia, widely grown for its edible bean, which has numerous uses. Furthermore, it is used as a raw material in the soy sauce industry, and its protein and antioxidant content is preferred to yellow soybeans. This study was conducted to evaluate the in vitro callus growth pattern of four varieties of black soybean grown in various combinations of cytokinins and 2,4-dichlorophenoxyacetic acid (2,4-D), and was performed at the Agronomy Laboratory, Faculty of Agriculture, the University of Bengkulu. A completely factorial randomized design (RAL) with 10 replications was used in this experiment. The first factor was four varieties of black soybeans, namely Detam-1, 2, 3, and 4. Similarly, the second factor was the type of cytokinin, namely K0 = 0 (control / without plant growth regulators), K1 = 2 mg.L -1 BAP + 0.5 mg.L -1 2,4-D, and K2 = 2 mg.L -1 Kinetin + 0, 5 mg.L -1 2,4-D. The mature cotyledon explants were cultured on Murashige and Skoog (MS) Medium. The results showed that Detam 2, 3 and 4 black soybean varieties grown on MS + 2 mg.L -1 BAP + 0.5 mg. L -1 2,4-D medium produced more friable callus with clear green-yellow color as compared to those grown on MS + 2 mg.L -1 Kinetin + 0.5 mg.L -1 2,4-D. The greatest compact callus with yellow-green color were formed by Detam 1 variety planted on MS medium + 2 mg.L -1 Kinetin + 0.5 mg.L -1 2,4-D. The greatest embryogenic callus production (100%), the heaviest callus fresh weight (1.53 g) and the greatest fresh culture yield (1.58 g) of yellow-green callus with friable texture were produced by Detam 3 variety grown on MS + medium 2 mg.L -1 BAP + 0.5 mg.L -1 2,4-D.

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Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)

Cited by 5 publications

References 7 publications

Progress in Soybean Genetic Transformation Over the Last Decade

Progress in Soybean Genetic Transformation Over the Last Decade

Antimicrobial and In-vitro Cytotoxic Efficacy of Betulinic Acid from the Callus, Regeneration, and Mother Plant of Dillenia indica L.

The Induction of Callus in Four Diverse Black Soybeans (Glycine soja Sieb. & Zucc) with Various Combinations of Cytokinins and 2,4-Dichlorophenoxyacetinic Acid

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