Embryo production and endocrine response in ewes superovulated with PMSG, with or without monoclonal anti-PMSG administered at different times

Martemucci, G.; D’Alessandro, Angela Gabriella; Toteda, Francesco; Facciolongo, A. M.; Gambacorta, M.

doi:10.1016/0093-691x(95)00249-8

Cited by 8 publications

(2 citation statements)

References 21 publications

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“…It was developed and marketed for cattle to limit the adverse effects of PMSG to improve the embryo production after superovulation treatment (117, 118). Due to a long half-life, PMSG had the disadvantage to cause a prolonged stimulation of follicular growth after preovulatory LH peak, inducing a poor response to superovulation treatment for cattle (117, 119, 120), sheep (121), and goat (122). Neutra-PMSG® injected 1–2 days after PMSG in superovulation treatment neutralized the adverse effects of PMSG by reducing its half-life in systemic circulation, improving embryo production.…”

Section: Anti-gonadotropin Antibodies As Therapeutic Agentsmentioning

confidence: 99%

Modulation of Gonadotropins Activity by Antibodies

Kara¹,

Dupuy²,

Bouillon

et al. 2019

Front. Endocrinol.

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Gonadotropins are essential for reproduction control in humans as well as in animals. They are widely used all over the world for ovarian stimulation in women, spermatogenesis stimulation in men, and ovulation induction and superovulation in animals. Despite the availability of many different preparations, all are made of the native hormones. Having different ligands with a wide activity range for a given receptor helps better understand its molecular and cellular signaling mechanisms as well as its physiological functions, and thus helps the development of more specific and adapted medicines. One way to control the gonadotropins' activity could be the use of modulating antibodies. Antibodies are powerful tools that were largely used to decipher gonadotropins' actions and they have shown their utility as therapeutics in several other indications such as cancer. In this review, we summarize the inhibitory and potentiating antibodies to gonadotropins, and their potential therapeutic applications.

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Section: Anti-gonadotropin Antibodies As Therapeutic Agentsmentioning

confidence: 99%

Modulation of Gonadotropins Activity by Antibodies

Kara¹,

Dupuy²,

Bouillon

et al. 2019

Front. Endocrinol.

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“…However, eCG treatment may cause postovulatory follicular waves (6) along with a high level of steroid production (3). It has been proposed that an injection of anti-eCG antibody 1-2 days following eCG injection may minimize the adverse effects of eCG by lowering the half-life of eCG in systemic circulation, which may have beneficial effects on the ovulation rate, pregnancy rate and quality of em-bryos (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Production and Purification of Equine Chorionic Gonadotropin Hormone Using Polyclonal Antibody

Sharifi¹,

Kohram²,

Shahneh³

et al. 2014

Iran J Biotech

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Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Objectives: The aim of the current study was to develop an accurate and fast method for production and purification of eCG using a polyclonal antibody assay. Materials and Methods: The blood samples (300 mL) were taken from the jugular vein of 17 mares on days 50, 70 and 90 of pregnancy. Plasma of the samples was siphoned and phenol solution was added to the plasma and stored in the refrigerator until eCG extraction. To prepare the polyclonal antibody against eCG, four male rabbits, about four months old with 2 kg weight, were chosen. A basic immunization was done by injecting 25 IU of eCG to the rabbits. Ouchterlony assay or double immunodiffusion test was used to assess the immunization and the titer of antiserum against eCG. eCG was purified from the plasma via the solid-phase extraction (SPE) method. Results: Results based on agarose-gel double immunodiffusion test showed that rabbits were completely immunized. SDS-PAGE analysis showed purified eCG is extracted without any significant contamination. Conclusions: The extraction of eCG with polyclonal antibody using the SPE method and production of anti-eCG antiserum in rabbits is suitable and may be a cost effective method for large scale production of eCG and anti-eCG antiserum in Iran.

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Induction of Superovulation in Domestic Ruminants

Gonzalez

Calero

Beckers

Focus on Biotechnology

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Embryo production and endocrine response in ewes superovulated with PMSG, with or without monoclonal anti-PMSG administered at different times

Cited by 8 publications

References 21 publications

Modulation of Gonadotropins Activity by Antibodies

Modulation of Gonadotropins Activity by Antibodies

Production and Purification of Equine Chorionic Gonadotropin Hormone Using Polyclonal Antibody

Induction of Superovulation in Domestic Ruminants

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