Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials

Rosato, Antonio; Carocci, Alessia; Catalano, Alessia; Clodoveo, Maria Lisa; Franchini, Carlo; Corbo, Filomena; Carbonara, Giuseppe; Carrieri, Antonio; Fracchiolla, Giuseppe

doi:10.1371/journal.pone.0200902

Cited by 69 publications

(56 citation statements)

References 43 publications

(36 reference statements)

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“…Pure Mentha piperita EO was identified for 97.58% of its composition [ 21 , 22 ]. Menthol is the major component being the 67.98% of the whole EO followed by 1-menthone, representing the 17.87% of the mixture.…”

Section: Resultsmentioning

confidence: 99%

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Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics

et al. 2020

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In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of Cinnammonum zeylanicum, Mentha piperita, Origanum vulgare, and Thymus vulgaris EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin.

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Section: Resultsmentioning

confidence: 99%

“…In fact, the data obtained clearly show a significant reduction in the concentration of antibiotics when used in association with essential oils. In particular, this work emphasizes the efficacy of Cinnamomum zeylanicum EO associated with antibiotics, when it is compared with other common EOs studied [ 21 , 22 , 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics

et al. 2020

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“…Indeed some papers report an increase of susceptibility to antibiotics after treatment with essential oils 25,26 . Although a pletora of publications did not show development of resistance to EOs, a very recent publication suggested the induction of efflux pumps and multidrug resistance in P. aeruginosa by Cinnamaldehyde, the main component of cinnamon 27 .…”

Section: Discussionmentioning

confidence: 99%

Essential oils against bacterial isolates from cystic fibrosis patients by means of antimicrobial and unsupervised machine learning approaches

Ragno

Papa²,

Patsilinakos

et al. 2020

Sci Rep

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Recurrent and chronic respiratory tract infections in cystic fibrosis (CF) patients result in progressive lung damage and represent the primary cause of morbidity and mortality. Staphylococcus aureus (S. aureus) is one of the earliest bacteria in CF infants and children. Starting from early adolescence, patients become chronically infected with Gram-negative non-fermenting bacteria, and Pseudomonas aeruginosa (P. aeruginosa) is the most relevant and recurring. Intensive use of antimicrobial drugs to fight lung infections inevitably leads to the onset of antibiotic resistant bacterial strains. New antimicrobial compounds should be identified to overcome antibiotic resistance in these patients. Recently interesting data were reported in literature on the use of natural derived compounds that inhibited in vitro S. aureus and P. aeruginosa bacterial growth. Essential oils, among these, seemed to be the most promising. In this work is reported an extensive study on 61 essential oils (EOs) against a panel of 40 clinical strains isolated from CF patients. To reduce the in vitro procedure and render the investigation as convergent as possible, machine learning clusterization algorithms were firstly applied to pickup a fewer number of representative strains among the panel of 40. This approach allowed us to easily identify three EOs able to strongly inhibit bacterial growth of all bacterial strains. Interestingly, the EOs antibacterial activity is completely unrelated to the antibiotic resistance profile of each strain. Taking into account the results obtained, a clinical use of EOs could be suggested. Cystic fibrosis (CF), one of the most common lethal genetic disorders in Caucasian population, is inherited as an autosomal recessive disease and affects 70.000 persons worldwide (Cystic Fibrosis Foundation, CFF). The defective gene, identified in 1989, is the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) that is carried by 4% of persons (among Caucasians). Since CFTR encodes for a chloride channel of the epithelial cell surface, CF patients manifest a variety of multi-organ problems due to the alteration of sodium and chloride secretion across cell membranes and the subsequent luminal dehydration 1. The impairment of mucociliary clearance, which should remove all microbes entering the airways, leads to the production of a thick and dehydrated mucus in the CF lung, which promotes the airway chronic bacterial colonization 2. The microbiology of CF respiratory tract is peculiar. In the early stage of life, it is characterized by the prevalence of the Gram-positive bacterium Staphylococcus aureus (S. aureus). Overall, in 2017 more than half of affected individuals had at least one culture positive for methicillin sensitive S. aureus (MSSA). The highest prevalence of methicillin resistant S. aureus (MRSA) occurs in individuals between the ages of 10 and 30, while MSSA reaches the peak among patients younger than 10 (Cystic Fibrosis Foundation. 2017. Patient Registry Annual

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“…According to Rosato et al [40], the antibacterial activity in gram-negative bacteria occurs due to the high percentage of oxygenated monoterpenes present in the EO and consequently the synergism between these components. On the other hand, bacteria can also respond to adverse conditions in a transient way, through so-called stress tolerance responses.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

Chaves

Martins

Rodrigues

et al. 2019

Preprint

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This study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials

Cited by 69 publications

References 43 publications

Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics

Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics

Essential oils against bacterial isolates from cystic fibrosis patients by means of antimicrobial and unsupervised machine learning approaches

Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

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