Elucidation of bioactive compounds in hydroalcohol extract of Phyllanthus amarus Schum. and Thonn. leaf using GC-MS analysis

Ajayi, Gabriel O; Olorunrinu, Temitope J; Shittu, Muhin A

doi:10.31254/jsir.2020.9202

Cited by 7 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indole derivatives have different biological activities, i.e., anti-inflammatory, antiviral, antioxidant, anticancer and antimicrobial [28] . These results revealed that the chemical constituents of the methanol extract of P. emblica leaves are in agreement with previous studies reported by [29,30]…”

Section: Gc-ms Analysissupporting

confidence: 93%

In-vitro Antimicrobial Potentialities of Phylunthus emblica Leaf Extract against Some Human Pathogens

2021

View full text Add to dashboard Cite

Phyllanthus emblica is used in traditional medicine due to its bioactive constituents. This study aimed to evaluate the antimicrobial activity of the Phyllanthus emblica methanol extract and its chemical profile. The plant was extracted with 85% methanol and the total phenolic and flavonoid contents as well as antimicrobial activity on some Gram-positive, Gram-negative bacteria and some fungi species were evaluated. Also, GC-MS analysis of plant extract was administered. Phyllanthus emblica methanolic extract showed high total phenolic content (216.41± 2.1 mg GAE/g of dry extract) and total flavonoid content (32.31± 1.2 mg RE/g of dry extract). The antimicrobial activity of extract against some pathogenic microbes proved that the plant extract is effective against all species except Escherichia coli. The highest activity was showed against Aspergillus fumigatus & Syncephalastrum racemosum (fungi) as well as Staphylococcus aureus, Syncephalastrum racemosum & Streptococcus pneumonia (bacteria). GC-MS analysis of Phyllanthus emblica exhibited the presence of thirty-one bioactive compound. The major compounds are 1, 3-Dimethylindole (24.11%), Isopropyl palmitate (23.94%), Gamolenic acid (7.73%) 1, 5-Hexanediol (7.58%), 8, 11-Octadecadienoic acid methyl ester (6.66%), 2-Methylhexadecanoic acid (5.62%) and Linolenic acid (3.49%). Thus, Phyllanthus emblica may be used as a potential oral antibiotic against some pathogenic microbes.

show abstract

Section: Gc-ms Analysissupporting

confidence: 93%

In-vitro Antimicrobial Potentialities of Phylunthus emblica Leaf Extract against Some Human Pathogens

2021

View full text Add to dashboard Cite

show abstract

“…Analysis. Te analysis was conducted at the Scientifc and Technological Research Equipment Centre of Chulalongkorn University in Bangkok, Tailand [22]. GC-MS analysis involved an Agilent Technologies 19091S-433 gas chromatograph (GC) coupled with an Agilent 5973 mass selective detector (MSD), controlled by Agilent Chemstation software.…”

Section: Gas Chromatography-mass Spectrometric (Gc-ms)mentioning

confidence: 99%

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Limcharoen,

Chaniad,

Chonsut

et al. 2024

Advances in Pharmacological and Pharmaceutical Sciences

View full text Add to dashboard Cite

Krom Luang Chumphon Khet Udomsak remedy (KKR) has traditionally been used as an alternative treatment, particularly for hyperglycemia; however, its therapeutic efficacy has not been scientifically validated. Thus, this study aims to investigate the potential inhibitory and antioxidant effects of α-glucosidase enzyme and characterize the chemical profile of KKR extracts using gas chromatography-mass spectrometry (GC-MS). The investigation highlights both KKR extracts as potent inhibitors of α-glucosidase, with the ethanolic extract of KKR (KKRE) displaying an IC50 value of 46.80 µg/mL and a noncompetitive mode of action. The combination of ethanolic and aqueous extracts of KKR (KKRE and KKRA, respectively) with acarbose exhibited a synergistic effect against the α-glucosidase. The KKRE extract displayed strong scavenging effects in the DPPH assay (IC50 156.3 µg/mL) and contained significant total phenolic (172.82 mg GAE/g extract) and flavonoid (77.41 mg QE/g extract) contents. The major component of KKRE is palmitic acid (15.67%). Molecular docking revealed that the major compounds interacted with key amino acid residues (ASP215, GLU277, HIS351, ASP352, and ARG442), which are crucial for inhibiting α-glucosidase. Notably, campesterin had a more significant influence on α-glucosidase than acarbose, with low binding energy. These findings underscore the significance of KKR in traditional medicine and suggest that it is promising treatment for diabetes mellitus. Further studies using animal model will provide valuable insights for advancing this research.

show abstract

“…It is an important plant of the Indian Ayurvedic system of medicine, which is used in treating problems of the stomach, genitourinary system, liver, kidney, and spleen. Ajayi et al [48] analyzed multiple bioactive compounds in mg/100 g of leaf extract as phenol ( (3,4-dimethoxyphenyl)ethyl]-2-nitro-(12.68%), hexadecanoic acid, ethyl ester (12.63%), beta tocopherol (12.63%), and phytol (12.61%). The phytochemical concentrations in its leaves are 0.73 ± 0.01%, 1.85 ± 0.03%, 1.12 ± 0.01%, 1.80 ± 0.01%, 1.59 ± 0.50%, 0.13 ± 0.10%, and 0.86 ± 0.01%, for saponins, tannins, phenolics, anthocyanins, alkaloids, triterpenoids, and glycosides, respectively, while in the roots, they are are 0.91 ± 0.01%, 1.53 ± 0.03%, 0.70 ± 0.01%, 2.97 ± 0.10%, 2.47 ± 0.03%, 0.62 ± 0.01%, 0.90 ± 0.01%, and 2.02 ± 0.10% for saponins, tannins, phenolics, steroids, flavonoids, anthocyanins, glycosides, and coumarins, respectively [26].…”

Section: Phyllanthus Amarusmentioning

confidence: 99%

Ethnobotanical Review of Selected Medicinal Plants in Guam for the Treatment of Urinary Tract Ailments and Their Pharmacological Properties

Ferdosh

2023

Sci. Pharm.

View full text Add to dashboard Cite

The Chamorro healers of Guam have more than a thousand years of history of using herbs and medicinal plants for the treatment of common ailments. The objective of this study is to review the bioactive compounds and pharmacological properties of medicinal plants which are used for urinary tract health by local healers. Literature searches were performed using Google Scholar, ScienceDirect, PubMed, and SpringerLink, by using several keywords, including “medicinal plants in Guam”, “traditional uses”, “bioactive compounds”, “pharmacological properties”, and “urinary tract infections”. This review highlights the traditional uses, bioactive compounds, and pharmacological properties of five medicinal plant species, namely Euphorbia hirta, Phyllanthus amarus, Premna serratifolia, Psidium guajava, and Urena lobata. Phenolics, alkaloids, terpenoids, essential oils, and polypeptides are the leading secondary metabolites reported in different plant extracts, which have been found to have significant antimicrobial, antioxidant, anti-inflammatory, antidiabetic, and anticancer properties. The therapeutic claims made about medicinal plants in Guam are well supported by the literature, having similar applications and pharmacological properties in other regions of the world. These medicinal plants have a lot of unexplored potential that might be utilized to develop more potent drugs for the treatment of infectious diseases, as well as food and herbal supplements.

show abstract

Elucidation of bioactive compounds in hydroalcohol extract of Phyllanthus amarus Schum. and Thonn. leaf using GC-MS analysis

Cited by 7 publications

References 0 publications

In-vitro Antimicrobial Potentialities of Phylunthus emblica Leaf Extract against Some Human Pathogens

In-vitro Antimicrobial Potentialities of Phylunthus emblica Leaf Extract against Some Human Pathogens

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Ethnobotanical Review of Selected Medicinal Plants in Guam for the Treatment of Urinary Tract Ailments and Their Pharmacological Properties

Contact Info

Product

Resources

About