Elimination of the asymmetry in neutral-carrier based solvent polymeric membranes induced by proteins

Dürselen, Lucas F. J.; Wegmann, Dorothée; May, Karl; Oesch, U.; Simon, W.

doi:10.1021/ac00165a021

Cited by 56 publications

(35 citation statements)

References 6 publications

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“…The shifts depenc on the membrane composition, the history of the mem brane and on the serum sample; they can amount tc several mV. 2,16 Research for a suitable membrane composition foi serum assays resulted in an ISE PVC-membrant (poly(vinyl chloride)) with Hemisodium® as the liganc and bis(1-butylphenyl)adipate as the plasticizer. Thi optimized membrane shows rather low shifts of Eo when contacted with human serum which do not exceec 0.3 mV.…”

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confidence: 99%

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

et al. 1992

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The limitations of ion-selective electrodes (ISEs) presently in use mainly concern the stability of the calibration values. By applying optimal membrane technologies and optimized reference electrodes a perfectly symmetric measuring system including a symmetric ISE membrane has been developed. Thus, stable standard potentials of the cell assembly, both before and after serum contact, as well as theoretical slopes of the electrode response have been obtained. Results of calibration-free assays of K+ and Na+ concentrations in aqueous calibration solutions and in undiluted human serum are presented. The precision required in fully met for K+ measurements in aqueous solutions and in sera of the normal as well as pathological concentration range. In addition, the precision of Na+ assays in aqueous calibration solutions and in serum samples of the normal concentration range is close to the required values.

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confidence: 99%

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

et al. 1992

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“…It should be noted that Simon and coworkers have observed that the introduction of hydroxyl groups into the WC matrix (obtained by hydrolysis of a vinyl chloride-vinyl acetate copolymer) serves to prohibit protein adsorption when the electrodes are brought in contact with serum samples [7].…”

Section: Results and Discusionmentioning

confidence: 99%

“…Indeed, it is well established that protein may adsorb on the membrane surface of ion-selective electrodes and therefore affect the response of these sensors. This is usually accompanied by a shift in the standard cell potential [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of proteins on the response of anion‐selective electrodes based on vitamin B₁₂ derivatives

1991

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KmtWky 40506-0055, U.S.A. ABSTRACTThe potentiometric response of several polymer membrane based ion-selective electrodes prepared with hydrophobic vitamin B,, derivatives is described. The effect of different proteins on the properties of these electrodes is discussed. It is suggested that the observed changes in potential upon addition of proteins are due mainly to nonspecific interactions between the proteins and the polymer membrane. Reduction of the nonspecific binding effect is possible by using a polymer matrix that contains free hydroxyl groups. The observed results agree with a theoretical model, which predicts that the nonspecific binding effect is more evident when membranes loaded with relatively small amounts of ionophore are used. The implications of these observations for the development of biosensors are discussed.

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“…Not only were the values of drifcs and hystereses of ISFETs bigger than those of ISEs, but also deviations from the average values Smaller slopes and inferior selectivities of the membranes using ETH2112, also reported in the literature [21], have been attributed to low mobility of the ligand in the membrane phase. The hydroxy functionality of OH-PVC used in the present study was 17% [20]. This comparatively high functionality might be the reason for lower slopes and poorer selectivities of the membranes using OH-PVC.…”

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confidence: 79%

Comparative studies between ion‐selective field effect transistors and ion‐selective electrodes with polymeric membranes

Miyahara

Simon

1991

Electroanalysis

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Characteristics of ion-selective field effect transistors (ISFETs) and ion-selective electrodes (ISEs) with solvent polymeric membranes were compared for slope, selectivity, drift, and hysteresis. Drift and hysteresis of ISFETs were significantly inferior to those of analogous ISEs. Experiments using conventional ISEs with internal electrolyte solutions and ion-selective liquid membranes of different kinds indicate that both primary ion and ionophore are necessary in the internal electrolyte solution and the ionselective membrane, respectively, to achieve small drift and small hysteresis. It is, therefore, concluded that to relllize highly reliable membrane-solid contact devices, the interface between the membrane and the solid in membrane-solid contact devices like ISFETs and coated wire electrodes (CWEs) should be electrochemically defined.

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Elimination of the asymmetry in neutral-carrier based solvent polymeric membranes induced by proteins

Cited by 56 publications

References 6 publications

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

Effect of proteins on the response of anion‐selective electrodes based on vitamin B₁₂ derivatives

Comparative studies between ion‐selective field effect transistors and ion‐selective electrodes with polymeric membranes

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Elimination of the asymmetry in neutral-carrier based solvent polymeric membranes induced by proteins

Cited by 56 publications

References 6 publications

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

Calibration-Free Measurement of Sodium and Potassium in Undiluted Human Serum with an Electrically Symmetric Measuring System

Effect of proteins on the response of anion‐selective electrodes based on vitamin B12 derivatives

Comparative studies between ion‐selective field effect transistors and ion‐selective electrodes with polymeric membranes

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Product

Resources

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Effect of proteins on the response of anion‐selective electrodes based on vitamin B₁₂ derivatives