Elevation of cytochrome P450-immunopositive protein and DNA damage in mussels (Mytilus edulis) transplanted to a contaminated site

Shaw, J; Large, Andrew; Livingstone, David; Doyotte, A; Renger, J; Chipman, J.K.; Peters, L.D.

doi:10.1016/s0141-1136(02)00191-5

Cited by 23 publications

(8 citation statements)

References 9 publications

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“…Many studies have examined total microsomal cytochrome P450 content, levels of CYP enzyme activity, or CYP protein by immunoassay in bivalve mollusks exposed to chemicals (Canova et al, 1998; Livingstone, 1998; Livingstone et al, 1989; Monari et al, 2007; Monari et al, 2009; Shaw et al, 2002; Snyder et al, 2001; Sole and Livingstone, 2005; Wootton et al, 1995). The interpretation of those measurements has been complicated by low catalytic activity of microsomal enzymes with diagnostic substrates for CYP1A-like activity, such as ethoxyresorufin (ethoxyresorufin-O-deethylase activity, EROD) and benzo[a]pyrene (benzo[a]pyrene hydroxylase; BPH), and lack of specific cross-reactivity of antibodies for CYP1As, (Chaty et al, 2004; Jonsson et al, 2006a; Jonsson et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

et al. 2013

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Various sequencing projects over the last several years have aided the discovery of previously uncharacterized invertebrate sequences, including new cytochrome P450 genes (CYPs). Here we present data on the identification and characterization of two CYP1-like and three CYP3-like genes from the bivalve mollusk Mytilus edulis, and assess their potential as biomarkers based on their responses to several known vertebrate aryl hydrocarbon receptor (AHR) agonists. Quantitative real-time PCR was used to measure CYP transcript levels in digestive gland, labial palps, adductor muscle, gill, foot, and different regions of the mantle. Levels of both CYP1-like genes were highest in digestive gland, whereas labial palps had the highest expression levels of the three CYP3-like genes followed by digestive gland and outer margin of the mantle. Mussels were exposed by injection to the AHR agonists, β-naphthoflavone (BNF; 25 μg.g−1), 3,3′,4,4′,5-polychlorinated biphenyl (PCB126; 2 μg.g−1), or 6-formylindolo[3,2-b]carbazole (FICZ; 0.1 μg.g−1), or to Aroclor 1254 (a mixture of PCBs; 50 μg.g−1) for 24 hours, followed by CYP expression analysis. There was no statistically significant change in expression of either of the CYP1-like genes after exposure to the various AHR agonists. The CYP3-like-1 gene was significantly up-regulated by BNF in gill tissues and the CYP3-like-2 gene was up-regulated in digestive gland by PCB126 and in gill tissue by BNF. These results suggest that distinct mechanisms of CYP gene activation could be present in M. edulis, although the importance of the CYP1-like and CYP3-like genes for xenobiotic and endogenous lipids biotransformation requires additional investigation.

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Section: Introductionmentioning

confidence: 99%

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

et al. 2013

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“…ROS production also entailed lipid peroxidation and lipid metabolism disruption (Ferreira and Vale, 1998), and reproductive irregularities if germ cells are affected or the hormonal balance is perturbed (Armstrong, 1990). The correlation of DNA damage with cyp450 induction is well described in marine molluscs, like the mussels Mytilus galloprovincialis and Mytilus edulis (Canova et al, 1998;Shaw et al, 2002). These studies were conducted using heterolog mRNA probes and antibodies developed in fishes (Shaw et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The correlation of DNA damage with cyp450 induction is well described in marine molluscs, like the mussels Mytilus galloprovincialis and Mytilus edulis (Canova et al, 1998;Shaw et al, 2002). These studies were conducted using heterolog mRNA probes and antibodies developed in fishes (Shaw et al, 2002). To our knowledge, no sequence for either cDNA or gene encoding Cyp450 in molluscan species was in the available databases.…”

Section: Introductionmentioning

confidence: 99%

Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions

Boutet

Tanguy

Moraga

2004

Gene

176

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“…The induction of this enzyme activity occurred at the same time that total PAHs was accumulated in mussels' tissues to concentrations 2.5-fold higher. Similarly, an increase of CYP1A protein up to 1.4-fold and an increased of the DNA damage (1.2-fold) occurred in mussels M. edulis transplanted from Port Quinn (reference site) to New Brighton (urban/industrial site, with high PAHs and PCBs levels) in the UK, after 13 weeks (Shaw et al 2002). Peters et al (1998) reported a preferential elevation of CYPlA-immunopositive protein with 3 weeks transplantation from a relatively clean site to a site containing 3-6 fold higher tissue levels of PAHs and PCBs is consistent with the observations of increased digestive gland CYPlA-immunopositive protein in Mytilus sp.…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 92%

“…In recent years, many studies highlighted the importance of using an integrated approach to assess the effects of environmental changes in coastal or estuarine zones (Peters et al , 1999Shaw et al 2002). The application of the active biomonitoring (ABM) strategy, in which organisms of a clean or reference site are transplanted to the site that is being monitored, gives the advantage of assuring comparable results, reducing the variability normally found in field studies .…”

Section: Introductionmentioning

confidence: 99%

Responses of CYP450 dependent system to aliphatic and aromatic hydrocarbons body burden in transplanted mussels from South coast of Portugal

2011

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Mussels Mytilus galloprovincialis were cross-transplanted at South Portugal from a reference site (site 1) to a site more contaminated with hydrocarbon compounds (site 2), and vice versa, in an active biomonitoring (ABM) concept, to assess the biotransformation capacity catalyzed by the mixed function oxygenase (MFO) system. Total alkanes (TAlk), the unresolved complex mixture (UCM), and total polycyclic aromatic hydrocarbons (TPAHs) concentration increased respectively 6, 4.4 and 4.2 fold relatively to control, in mussels transplanted from site 1 to 2. In the cross-transplant, a 48, 57 and 62% depuration of TAlk, UCM and TPAHs concentrations occurred by the end of the 3-4th week. Petrogenic and biogenic (marine and terrigenous) sources of AHs, and petrogenic and pyrolitic (biomass and oil/fuel incomplete combustion) sources of PAHs were detected at both sites. CYP450, CYT b (5) and NADPH-RED in mussels transplanted from site 1 to 2 were induced from day 0 to 28, with a total increase of 35, 32 and 35%, respectively, while biochemical equilibrium to lesser environmental contamination occurs in mussels transplanted from site 2 to 1. A significant relationship between CYP450 and NADPH-RED was found with TPAH, with distinctive behavior at the two sites. MFO system components increase with exposure time at one site and decreases in the other, reflecting an adaptation to distinct environmental hydrocarbon loads. The ABM strategy proved to be useful to understand the environment real impact on the biochemical responses in mussels' local populations. In this study, CYP450 and NADPH-RED are a useful biomarker for hydrocarbon exposure.

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Elevation of cytochrome P450-immunopositive protein and DNA damage in mussels (Mytilus edulis) transplanted to a contaminated site

Cited by 23 publications

References 9 publications

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions

Responses of CYP450 dependent system to aliphatic and aromatic hydrocarbons body burden in transplanted mussels from South coast of Portugal

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