Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions

Boutet, Isabelle; Tanguy, Arnaud; Moraga, Dario

doi:10.1016/j.gene.2003.12.027

Cited by 176 publications

(107 citation statements)

References 36 publications

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“…In bumblebee Bombus ignites, the level of icCu/Zn-SOD mRNA increased 3-fold at 6 h post-LPS stimulation compared with the control [31]. Similar expression profile for icCu/Zn-SOD was also found in bay scallop Argopecten irradians [23] and oyster C. gigas [32] with Vibrio or hydrocarbon stimulation. But for C. farreri, the expression level of icCu/Zn-SOD decreased to the lowest levels until 16 h post-Listonella anguillarum challenge, and then recovered [19].…”

Section: Time-course Expression Of Vpsod After Vibrio Challengesupporting

confidence: 59%

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Li¹,

Sun²,

Chen³

et al. 2010

Fish & Shellfish Immunology

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Section: Time-course Expression Of Vpsod After Vibrio Challengesupporting

confidence: 59%

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Li¹,

Sun²,

Chen³

et al. 2010

Fish & Shellfish Immunology

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“…Cu/Zn SOD genes have been cloned from several aquatic species including frog, Xenopus laevis [16], grouper, Epinephelus malbaricus [17], Pacific oyster, Crassostrea gigas [18] and the abalones Haliotis discus discus [4]. So far, the Cu/Zn SOD from pearl oysters has not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Anju

Jeswin

Thomas

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tBecause of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame (ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at 36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental pollution and cellular stress in pearl oyster.

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“…Those authors suggest that this difference in expression may be explained, at least in part, by the different mechanisms of detoxification that are triggered in response to different stressing agents. Boutet et al 32 have also described the observation that the presence of bicarbonates in C. macropomum causes a change in energy metabolism and regulation of glu activity. Many xenobiotic agents can also affect carbohydrate metabolism, causing an increase in energy consumption, which may lead to an increase in the ability of enzymes to metabolize proteins involved in catabolism for glucose production.…”

Section: Resultsmentioning

confidence: 98%

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

2013

Gene Expression to Genetical Genomics

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Abstract:In this work the authors evaluated the expression of cat, glu, cypP450, hsp70 and 18S RNA in Colossoma macropomum following exposure to copper and cadmium by semi-quantitative RT-PCR and quantitative qRT-PCR. In RT-PCR and qRT-PCR hsp70 expression was highest after exposure to copper for 3 hours (h). glu expression increased after exposure to cadmium, regardless of time. cat expression increased after the first hour of exposure to both metals, and decreased after 3 hs. cyp450 expression was higher when compared to controls in exposed fish regardless of metal and exposure time. cyp450 expression after exposure to copper for 3 h was twice that recorded after 1 h. In the presence of cadmium, cyp450 expression was higher in the animals exposed for 1 h and lower at 3 h. Therefore, toxicology tests and biomonitoring based on molecular markers should focus on a set of molecular and/or environmental parameters, rather than isolated markers.

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Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions

Cited by 176 publications

References 36 publications

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

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