Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Li, Chenghua; Sun, Han‐Dong; Chen, Aiqin; Ning, Xuanxuan; Wu, Huifeng; Qin, Song; Xue, Qinzhao; Zhao, Jianmin

doi:10.1016/j.fsi.2009.11.021

Cited by 54 publications

(30 citation statements)

References 31 publications

(18 reference statements)

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“…Nested PCR strategy was employed to clone the 3 0 and 5 0 ends of VpSe-GPx. The RNA extraction, cDNA synthesis and PCR amplification were performed according to previously described [24].…”

Section: Cloning the Full-length Cdna Of Vpse-gpxmentioning

confidence: 99%

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Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Zhang

Liu

Chen

et al. 2011

Fish & Shellfish Immunology

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“…Nested PCR strategy was employed to clone the 3 0 and 5 0 ends of VpSe-GPx. The RNA extraction, cDNA synthesis and PCR amplification were performed according to previously described [24].…”

Section: Cloning the Full-length Cdna Of Vpse-gpxmentioning

confidence: 99%

“…In a 96-well plate, each sample was run in triplicate along with the internal control. The RNA extraction, cDNA synthesis, reaction component, thermal profile and the data analysis were conducted as previously described [24]. All datasets were given in terms of relative mRNA expression as means AE S.D.…”

Section: Sequence Analysismentioning

confidence: 99%

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Zhang

Liu

Chen

et al. 2011

Fish & Shellfish Immunology

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“…One microgram of total RNA was used for cDNA synthesis according to the manufacture's protocol (Promega, USA). qRT-PCR was carried out in an ABI 7500 Real-time Detection System by using the SYBR ExScript qRT-PCR Kit (Takara) as described previously (Li et al, 2010). The expression level of VpTrx1 and VpTrp14 was analyzed using 2 − ΔΔCT method (Livak and Schmittgen, 2001) with β-actin mRNA as the control (Li et al, 2010).…”

Section: Tissue-specific Expression Of Vptrx1 and Vptrp14 Mrnamentioning

confidence: 99%

“…qRT-PCR was carried out in an ABI 7500 Real-time Detection System by using the SYBR ExScript qRT-PCR Kit (Takara) as described previously (Li et al, 2010). The expression level of VpTrx1 and VpTrp14 was analyzed using 2 − ΔΔCT method (Livak and Schmittgen, 2001) with β-actin mRNA as the control (Li et al, 2010). The primers used to amplify the β-actin gene were P1 (5′-CTCCCTTGAGAAGAGCTACGA-3′) and P2 (5′-GATACCAGCAGATTC CATACCC-3′).…”

Section: Tissue-specific Expression Of Vptrx1 and Vptrp14 Mrnamentioning

confidence: 99%

Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum

Wang

Ning

Chen

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…In a 96-well plate, each sample was run in triplicate along with the internal control. The hepatopancreas RNA extraction, cDNA synthesis, reaction component, thermal profile and the data analysis were conducted as previously described [28]. Dissociation curve analysis of amplification products was performed at the end of each PCR reaction to confirm that only one PCR product was amplified and detected.…”

Section: Quantification Analysis Of Mrna Expressionmentioning

confidence: 99%

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

You

Ning²,

Liu³

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mg/L Cd 2þ -treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mg/L Cd 2þ for 24 h and 96 h.These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.

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Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Cited by 54 publications

References 31 publications

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

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