The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

Abstract

a b s t r a c tHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mg/L Cd 2þ -treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mg/L Cd 2þ for 24 h and 96 h.These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.

Rapid climate-driven evolution of the invasive species Mytilus galloprovincialis over the past century

“…Among these candidate adaptive loci, two markers have previously been associated with stress tolerance. Heat shock 70 kDa protein 12A (HSPA12A) possibly plays an important role in the mediation of the environmental stress in M. galloprovincialis (You et al 2013). HSPA12A is a novel and atypical member of the HSP70 family, possibly with a highly specialized function that may go beyond the HSP chaperone function.…”

Association of single nucleotide polymorphism of Hsp90ab1 gene with thermotolerance and milk yield in Sahiwal cows

“…Author(s) agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Cellular tolerance to heat stress is mediated by a family of proteins named heat shock proteins (HSPs). HSPs play essential roles in the immunity of organisms, particularly in relation to heat-resistance (Song et al, 2006;You et al, 2013). The chaperone, Hsp90 is one of the most abundant proteins in eukaryotic cells, comprising 1-2% of cellular proteins under non-stress conditions (Craven et al, 1996).…”

Polymorphisms in Hsp90ab1 gene and their association with heattolerance in Sahiwal and Karan Fries cows

“…Mammals respond to heat stress by transcriptional activation of a set of proteins known as heat shock proteins (Kregel, 2002). Heat shock proteins (Hsps) play essential roles in immunity of organisms, particularly in relation to heatresistance (Song et al, 2006, You et al, 2013. Therefore, identification for SNP markers associated with heatresistance has mainly concentrated on Hsps as the most suitable candidate genes (Rako et al, 2007;Yang et al, 2011;Chen et al, 2013).…”