Elevated urinary levels of 8-oxo-2′-deoxyguanosine, (5′R)- and (5′S)-8,5′-cyclo-2′-deoxyadenosines, and 8-iso-prostaglandin F2α as potential biomarkers of oxidative stress in patients with prediabetes

Kant, Melis; Akış, Merve; Çalan, Mehmet; Arkan, Tuğba; Bayraktar, Fırat; Dizdaroğlu, Miral; İşlekel, Hüray

doi:10.1016/j.dnarep.2016.09.004

Cited by 37 publications

(26 citation statements)

References 41 publications

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“…The prospective inverse correlation between baseline 8-OHdG and HOMA-β at the 3-year follow-up suggests that oxidative DNA damage might participate in β-cell dysfunction, which is consistent with a previous finding of pancreatic β-cells being particularly vulnerable to oxidative damage [37]. The levels of 8-OHdG, a well-known biomarker of DNA oxidation [38], were demonstrated to be elevated in prediabetes and in patients with T2D in numerous previous retrospective studies [39,40]. Unexpectedly, we found a modest inverse correlation between baseline 8-OHdG and PG120min at the 3-year follow-up, suggesting that elevated 8-OHdG might predict or contribute to decreased postload plasma glucose, which seemingly contradicts the previous findings.…”

supporting

confidence: 90%

Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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Cellular aging markers, including telomere length and mitochondrial function, as well as oxidative stress and inflammation markers influence each other and form a complex network, which is affected in diabetes. However, it remains unknown whether these markers could independently predict future diabetes after adjustment for their mutual effects. We conducted a 3-year longitudinal study in a Chinese cohort that comprised 108 nondiabetic individuals at baseline. The 2-hour 75 g oral glucose tolerance tests were performed at baseline and at 3-year follow-up. At baseline, leukocyte telomere length (LTL) and mitochondrial DNA copy number (mtDNAcn) in leukocytes were determined using the polymerase chain reaction method. Tumor necrosis factor (TNF-α), interleukin-6, 8-hydroxy-2-deoxyguanosine levels, and superoxide dismutase (SOD) activity were measured by the enzyme-linked immunosorbent assay. Participants who developed diabetes at the 3-year follow-up (n=28) had shorter LTL and higher levels of TNF-α and SOD activity at baseline. Baseline LTL was found to be independently associated with the development of diabetes at the 3-year follow-up after the adjustment for mtDNAcn, markers of oxidative stress and inflammation, and conventional diabetes risk factors. Our findings suggest that LTL is an independent predictor for 3-year diabetes risk, which might inform timely prevention and treatment of diabetes. Telomere shortening might be involved in the pathogenesis of diabetes independently of conventional diabetes risk factors, mtDNAcn, or oxidative stress and inflammation pathways.

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supporting

confidence: 90%

Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…However, in our study this marker was found to be significantly decreased in serum in both DM and No-DM patients in inverse relation to published reports where they found increased levels in urine [18]. Kant et al mentioned that when lipid peroxidation products were elevated in the prediabetic stage, the determination of this marker is useful in the detection of patients at risk of type 2 DM [28]. In this study, the 8-IP in residual urine was not measured.…”

Section: Discussionsupporting

confidence: 63%

Increase of Oxidants and Antioxidant Consumption in Patients with Type 2 Diabetes Mellitus in Peritoneal Dialysis

Pazarín-Villaseñor¹,

Gutierrez-Prieto²,

Soto-Vargas³

et al. 2019

Antioxidants

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Oxidative stress (OS) is implicated as a unifying factor between chronic kidney diseases and cardiovascular diseases. The objective of the study was to compare the oxidant and antioxidant status in patients with PD according to the state of DM. Lipoperoxides (LPO), 8-isoprostanes (8-IP) and nitric oxide (NO) were determined as oxidants and the activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) as antioxidants in patients with DM and without DM (No-DM). We included 35 patients with DM, 42 No-DM patients and 10 healthy people as a control group (HC). Patients with DM were older (p<0.0001), had higher BMI (p<0.0001), high glucose levels (p<0.0001) and more hypertension (p<0.0001). It was found that LPO levels increased in patients with DM and No-DM vs. HC (p<0.0001). There was a decrease in the levels of 8-IP in DM and No-DM compared to HC (p<0.0001). The levels of NO in patients with DM and No-DM decreased significantly compared to the HC group with 197.97±34.20 μM (p<0.0001). The activity of the SOD enzyme in patients with DM and No-DM was found to be increased compared to the HC group (p<0.0001). The levels of TAC in HC were 2.62±0.17 mM and decreased in patients with DM and No-DM (p<0.0001).

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“…High level of DNA oxidation, measured as urinary excretion of 8oxodG, is predictive for the risk of breast and lung cancer, atherosclerosis, and diabetes [176–179]. RNA oxidation, measured as 7,8-dihydroxy-8-oxoguanosine (8oxoGuo), has been recently introduced as a marker of diseases, particularly neurodegeneration and diabetes, and high level of RNA oxidation has been also associated with breast cancer development in diabetic females [180].…”

Section: Markers Based On Ros-induced Modificationsmentioning

confidence: 99%

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

Marrocco

Altieri

Peluso

2017

Oxidative Medicine and Cellular Longevity

611

500

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Oxidative stress is the result of the imbalance between reactive oxygen species (ROS) formation and enzymatic and nonenzymatic antioxidants. Biomarkers of oxidative stress are relevant in the evaluation of the disease status and of the health-enhancing effects of antioxidants. We aim to discuss the major methodological bias of methods used for the evaluation of oxidative stress in humans. There is a lack of consensus concerning the validation, standardization, and reproducibility of methods for the measurement of the following: (1) ROS in leukocytes and platelets by flow cytometry, (2) markers based on ROS-induced modifications of lipids, DNA, and proteins, (3) enzymatic players of redox status, and (4) total antioxidant capacity of human body fluids. It has been suggested that the bias of each method could be overcome by using indexes of oxidative stress that include more than one marker. However, the choice of the markers considered in the global index should be dictated by the aim of the study and its design, as well as by the clinical relevance in the selected subjects. In conclusion, the clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions.

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Elevated urinary levels of 8-oxo-2′-deoxyguanosine, (5′R)- and (5′S)-8,5′-cyclo-2′-deoxyadenosines, and 8-iso-prostaglandin F2α as potential biomarkers of oxidative stress in patients with prediabetes

Cited by 37 publications

References 41 publications

Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population

Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population

Increase of Oxidants and Antioxidant Consumption in Patients with Type 2 Diabetes Mellitus in Peritoneal Dialysis

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

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