Elevated mitochondrial biogenesis in skeletal muscle is associated with testosterone‐induced body weight loss in male mice

Abstract

a b s t r a c tAndrogen reduces fat mass, although the underlying mechanisms are unknown. Here, we examined the effect of testosterone on heat production and mitochondrial biogenesis. Testosterone-treated mice exhibited elevated heat production. Treatment with testosterone increased the expression level of peroxisome proliferator-activated receptor-c coactivator-1a (PGC1a), ATP5B and Cox4 in skeletal muscle, but not that in brown adipose tissue and liver. mRNA levels of genes involved in mitochondrial biogenesis were elevated in skeletal muscle isolated from testosterone-treated male mice, but were down-regulated in androgen receptor deficient mice. These results demonstrated that the testosterone-induced increase in energy expenditure is derived from elevated mitochondrial biogenesis in skeletal muscle.

Proteome-wide muscle protein fractional synthesis rates predict muscle mass gain in response to a selective androgen receptor modulator in rats

“…There are conflicting data on the effect of testosterone on muscle mitochondrial biogenesis. Usui et al (28) demonstrated that in mice, testosterone treatment resulted in an increase in heat production that the authors concluded was derived from elevated mitochondrial biogenesis in skeletal muscle. On the other hand, in hypogonadal men treated with testosterone, Petersson et al (19) observed no effect on insulin sensitivity or skeletal muscle mRNA levels of genes involved in mitochondrial biogenesis, oxidative phosphorylation, or lipid metabolism.…”

Testosterone Attenuates Age-Related Fall in Aerobic Function in Mobility Limited Older Men With Low Testosterone

“…This measure, indexed as peak oxygen uptake (V O 2 peak), is an important assessment of physical performance as it identifies a person's ability to perform work over prolonged periods of time, a fundamental requirement for daily physical functioning and maintenance of independence (9). Testosterone administration increases red cell mass, 2,3 bisphosphoglycerate, muscle fiber capillarity (10) and as shown in mice, mitochondrial biogenesis (11,12), all of which would be expected to increase tissue O 2 delivery and uptake. However, most earlier reports of testosterone's effects on aerobic performance in young healthy men (13,14) and male athletes (15) have not provided compelling evidence of testosterone's effect on aerobic performance.…”

Effect of Conjugated Linoleic Acid Intake on Endurance Exercise Performance and Anti-fatigue in Student Athletes

“…On the other hand, increased muscle mass by CLA intake was suggested by increased serum testosterone level 15,24,27 . Testosterone can induce increase of energy expenditure by elevating mitochondrial biogenesis in skeletal muscle in mice 28 . As previously mentioned, CLA intake has been reported to improve steroidogenesis, muscle hypertrophy, physical activity, and endurance capacity in mice 3 .…”