Elevated levels of tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis

Nakajima, Yasuyuki; Mikami, Osamu; Yoshioka, Miyako; Motoi, Yoshiko; Ito, Takashi; Ishikawa, Yoshiharu; Fuse, M.; Nakano, Kayoko; Yasukawa, Kiyoshi

doi:10.1016/s0034-5288(97)90209-5

Cited by 72 publications

(57 citation statements)

References 13 publications

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“…Increased TNFα concentrations in milk have been reported in earlier studies of experimental LPS [3,10,22] or E. coli mastitis [3,8,10,28] and in spontaneous E. coli mastitis [9,19,20]. In serum, no TNFα has been found or the levels have been very low [3,8,10,19,20,22,28]. Differences in TNFα may be the result of variations in the study protocols and sample treatments between the studies.…”

Section: Discussionmentioning

confidence: 87%

“…IL-6 increases in the serum after LPS stimulation or E. coli mastitis [19,27], and it has been shown to be a potent inducer of hepatic production of SAA [2]. In milk, concentrations of TNFα and SAA seemed to be closely intertwined; cows with very low TNFα also had low SAA concentrations, and those with high TNFα had high SAA concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, at a later stage of inflammation the effect of TNFα may turn deleterious to the host if the production of TNFα increases to high levels [15]. Several authors have found correlations between the severity of response and concentrations of TNFα in the blood or milk [3,8,9,20]; in other studies, no direct correlation has been seen [10,19]. In LPS mastitis models, TNFα was detected in the plasma and milk of early lactating cows [3,10], but not in cows in midlactation [22].…”

Section: Introductionmentioning

confidence: 99%

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Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

2004

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-A cross-over study was conducted to investigate the effect of intramammarily infused lipopolysaccharide (LPS) on the acute phase reaction in early (EL) and in late (LL) lactation. Nine cows received intramammary injections of 100 µg of Escherichia coli 0111:B4 LPS during EL and LL. The severity of each cows systemic and local signs and change in milk appearance were recorded and scored throughout the experiment. Systemic and local signs were found to be more serious in EL cows. Tumor necrosis factor α (TNFα) was detected in milk but not in serum. Serum amyloid A (SAA) concentrations increased both in serum and in milk. The milk TNFα concentrations peaked at 8 h post-challenge (PC). SAA concentrations started to increase at 8 h PC, and peak concentrations were seen at 32 and 48 h PC in milk and serum, respectively. The milk TNFα and SAA seemed to be correlated, being on average higher in EL. Serum SAA concentration was not correlated with milk TNFα or SAA, nor with the severity of local or systemic signs, but was correlated with changes in milk appearance. LPS / mastitis / dairy cow / SAA / TNFα

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

2004

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“…Therefore, determination of cytokine levels during the course of mastitis is thought to be useful for elucidating the pathogenesis of mastitis. Although there have been several studies on cytokines in experimental and naturally occurring mastitis of cattle [10,11,13,14,16,17,19,20], it is very difficult to review the kinetics and to clarify the significance of cytokine production in the course of mastitis from these studies due to factors such as limited kinds of cytokines studied, various assay methods, and different observation periods.…”

mentioning

confidence: 99%

“…The observations of the present study support this finding at the protein level. Increased TNF-α levels in whey [11,13,14,16] and presence of the relatively low (less than 10 ng/ml) concentrations of TNF-α in sera [10,20] from experimental and natural cases of coliform or endotoxin-induced mastitis have been reported. These findings also coincide with the present observations.…”

mentioning

confidence: 99%

Interferon-.GAMMA. and Tumor Necrosis Factor-.ALPHA. Levels in Sera and Whey of Cattle with Naturally Occurring Coliform Mastitis.

Hisaeda¹,

Hagiwara

Eguchi

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Concentrations of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were determined in serum and whey samples from cattle with naturally occurring coliform mastitis for two weeks after onset using bovine INF-γ-and TNF-α-specific ELISA. In serum and whey samples from healthy cows, IFN-γ was almost undetectable and TNF-α was detected at low levels. At the onset of illness, INF-γ in sera and whey and TNF-α in whey from the mastitic cows were significantly higher than their respective values in healthy cows. Concentrations of IFN-γ and TNF-α in whey from mastitic cattle decreased significantly as the cows recovered. KEY WORDS: bovine mastitis, interferon-γ, tumor necrosis factor-α.

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Tumor necrosis factor alpha increases P‐glycoprotein expression in a BME‐UV in vitro model of mammary epithelial cells

Al‐bataineh

Merwe

Schultz

et al. 2010

Biopharm & Drug Disp

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P-glycoprotein is an efflux pump belonging to the ATP-binding cassette super-family that influences the bioavailability and disposition of many drugs. Mammary epithelial cells express various drug transporters including P-glycoprotein, albeit at low level during lactation. During inflammatory reactions, which can be associated with changes in epithelial barrier functions, pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α) are elevated in milk and serum. In this study, the role of TNF-α in the regulation of P-glycoprotein was determined in cultured BME-UV cells, an immortalized bovine mammary epithelial cell line. The protein production of P-glycoprotein and mRNA expression of bABCB1, the gene encoding P-glycoprotein, were increased after 24 hours of TNF-α exposure. The highest observed effects for TNF-α on the regulation of P-glycoprotein was after 72 hours of exposure. Protein and mRNA expression also significantly increased after 120 hours of TNF-α exposure, but it was lower than the level that observed in the cells exposed to TNF-α for 72 hours. The apical to basolateral flux of digoxin, a P-glycoprotein substrate, was decreased in the TNF-α-exposed epithelium. This effect was reversed when verapamil or ketoconazole, compounds known to interact with P-glycoprotein, were added together with digoxin into the donor compartment. Probenecid, a compound known to interact with organic anion transporters, but not P-glycoprotein, did not increase the flux of digoxin. This model has important implications for understanding the barrier function of the mammary epithelium and provides insight into the role of P-glycoprotein in the accumulation and/or removal of xenobiotics from milk and/or plasma.

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Elevated levels of tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis

Cited by 72 publications

References 13 publications

Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

Interferon-.GAMMA. and Tumor Necrosis Factor-.ALPHA. Levels in Sera and Whey of Cattle with Naturally Occurring Coliform Mastitis.

Tumor necrosis factor alpha increases P‐glycoprotein expression in a BME‐UV in vitro model of mammary epithelial cells

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