Serum amyloid A and TNF<b>?</b> in serum and milk during experimental endotoxin mastitis

Lehtolainen, Tanja; Røntved, Christine M.; Pyörälä, Satu

doi:10.1051/vetres:2004043

Cited by 53 publications

(41 citation statements)

References 34 publications

(75 reference statements)

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“…This is associated with several non-specific immune parameter changes, such as decreased respiratory burst and decreased nitric oxide concentration after calving (Piccinini et al, 2004). In cows infected with mastitis, the concentrations of haptoglobin, AGP, and serum amyloid A in serum were significantly increased as compared to those of healthy cows (Eckersall et al, 2001;Lehtolainen et al, 2004). Our results were consistent with previous observations that haptoglobin in plasma was increased at parturition in cows with and without mastitis but haptoglobin continued to be elevated 21 days postpartum in cows with subclinical mastitis.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Yang¹,

Wang²,

Bu³

et al. 2012

CZECH J ANIM SCI

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ABSTRACT:The transition period is the most critical time of the cow's lactation cycle that is associated with the onset of mastitis. In this study, changes of plasma proteins in cows (n = 12) with or without subclinical mastitis after calving were determined by two-dimensional electrophoresis (2-DE), which detected 18 spots with variations in protein spots abundance. These spots were identified by liquid chromatography coupled with tandem mass spectrometry. The changes in protein profile from day 21 before calving to day 1 after calving were similar in cows with or without subclinical mastitis. Abundance of α1 acid glycoprotein (AGP) and haptoglobin was dramatically increased at parturition, while transthyretin was down-regulated at parturition, and apolipoprotein E and immunoglobulin gamma 1 were up-regulated at postpartum compared with prepartum in periparturient dairy cows. In cows infected with subclinical mastitis, AGP, haptoglobin, and serum amyloid A were dramatically increased and continued to be elevated in plasma from day 1 to day 21 after calving compared with cows free of mastitis. Changes of protein in plasma at parturition may serve as an immune system response to parturition and lactation process at the protein level and suggest that these altered proteins would not serve as a potential marker for predicting if the periparturient dairy cows are susceptible to subclinical mastitis.

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Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Yang¹,

Wang²,

Bu³

et al. 2012

CZECH J ANIM SCI

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“…An ELISA for the quantification of bovine TNF-␣ in plasma (9) was modified for serum and milk as described by Lehtolainen et al (38). The milk samples were centrifuged twice at 25,000 ϫ g for 40 min at 4°C, and the clear supernatants of the milk were used for ELISA analysis.…”

Section: Methodsmentioning

confidence: 99%

Transgenic Cows That Produce Recombinant Human Lactoferrin in Milk Are Not Protected from Experimental Escherichia coli Intramammary Infection

et al. 2006

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This is the first study describing an experimental mastitis model using transgenic cows expressing recombinant human lactoferrin (rhLf) in their milk. The aim of the study was to investigate the concentrations in milk and protective effects of bovine and recombinant human lactoferrin in experimental Escherichia coli mastitis. Experimental intramammary infection was induced in one udder quarter of seven first-lactating rhLf-transgenic cows and six normal cows, using an E. coli strain isolated from cows with clinical mastitis and known to be susceptible to Lf in vitro. Clinical signs were recorded during the experimental period, concentrations of human and bovine Lf and indicators of inflammation and bacterial counts were determined for milk, and concentrations of acute-phase proteins and tumor necrosis factor alpha were determined for sera and milk. Serum cortisol and blood hematological and biochemical parameters were also determined. Expression levels of rhLf in the milk of transgenic cows remained constant throughout the experiment (mean, 2.9 mg/ml). The high Lf concentrations in the milk of transgenic cows did not protect them from intramammary infection. All cows became infected and developed clinical mastitis. The rhLf-transgenic cows showed milder systemic signs and lower serum cortisol and haptoglobin concentrations than did controls. This may be explained by lipopolysaccharide-neutralizing and immunomodulatory effects of the high Lf concentrations in their milk. However, Lf does not seem to be a very efficient protein for genetic engineering to enhance the mastitis resistance of dairy cows.

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“…During mastitis, SAA is found in markedly increased amounts in plasma, associated with high-density lipoproteins [69]. Rapid and large increases in SAA concentrations have been shown in milk from cows suffering from clinical mastitis but also from chronic subclinical mastitis [49,59,71,94,115]. Highly alkaline isoforms of SAA3 were demonstrated in milk from clinical E. coli mastitis, in much higher concentrations than the acidic isoforms of systemic origin [71].…”

Section: Inducible Defensesmentioning

confidence: 99%

Innate immunity of the bovine mammary gland

2006

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-Understanding the immune defenses of the mammary gland is instrumental in devising and developing measures to control mastitis, the major illness of dairy ruminants. Innate immunity is an extremely broad field for investigation, and despite decades of research, our present knowledge of the innate defenses of the udder is incomplete. Yet, information is being gained on the recognition of pathogens by the mammary gland, and on several locally inducible defenses. The contribution of mammary epithelial cells to local defenses and to the mobilization of leucocytes is under growing scrutiny. Interactions of mastitis-causing bacteria such as Escherichia coli or Staphylococcus aureus and the mammary gland represents a suitable model for studies on innate immunity at an epithelium frontier. Powerful new research tools are radically modifying the prospects for the understanding of the interplay between the mammary gland innate defenses and mastitis-causing bacteria: genetic dissection of the immune response, microarray gene technology, transcriptomic methodologies and gene silencing by RNA interference will make possible the discovery of several of the key defense mechanisms which govern the susceptibility/resistance to mastitis at the molecular and genetic levels. It should then be possible to enhance the resistance of dairy ruminants to mastitis through immunomodulation and genetic improvement. mastitis / cattle / innate immunity / inflammation / milk

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Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

Cited by 53 publications

References 34 publications

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Transgenic Cows That Produce Recombinant Human Lactoferrin in Milk Are Not Protected from Experimental Escherichia coli Intramammary Infection

Innate immunity of the bovine mammary gland

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