Elevated Cutaneous Leukocyte Concentration in a Rodent Model of Acute Venous Hypertension

Pathogenesis of primary chronic venous disease: Insights from animal models of venous hypertension

“…In acute experiments, anesthesia was maintained and experimental investigations began directly. 33 In the chronic model, the incisions were closed and the rats were maintained for 7 or 14 days before a second anesthesia and investigation. 34 Arterial and venous pressure measurements were obtained via cannulation of the right carotid artery and the right superficial epigastric vein.…”

“…Another rodent model, in which venous hypertension was produced by ligation of several large veins, [33][34][35] has been used to investigate the link between venous hypertension and the skin changes that are important manifestations of severe clinical CVD. 2…”

Evaluation of the Role of Intercellular Adhesion Molecule 1 in a Rodent Model of Chronic Venous Hypertension

“…MPO activity was assessed by measuring the H 2 O 2 -dependent oxidation of 3,3Ј,5,5Ј-tetramethylbenzidene, resulting in a blue chromogen detectable spectrophotometrically. In rats, 1 U of MPO activity equals 10 6 leukocytes in tissues [11,15].…”

“…After the final arterial and venous pressure readings, bilateral forelimb and hindlimb skin (epidermis, dermis, and subcutaneous fat) was harvested (ϳ2 ϫ 2-cm 2 sections), rinsed with saline, frozen in liquid nitrogen, and stored at Ϫ70°C until the MPO assay could be run. Arndt's myeloperoxidase assay [14], as previously described in our earlier work [11,12], was used to indirectly quantitate the number of leukocytes in the hindlimb skin of the animals. MPO activity was assessed by measuring the H 2 O 2 -dependent oxidation of 3,3Ј,5,5Ј-tetramethylbenzidene, resulting in a blue chromogen detectable spectrophotometrically.…”

“…We have developed both acute and chronic rodent models of obstructive VH in which we have demonstrated, using the myeloperoxidase (MPO) assay, a significant increase in the cutaneous leukocyte concentration in hypertensive hindlimbs [11,12].…”