Elementary chemical analysis in leaves infected by fumagina by X-Ray fluorescence technique

Blonski, Maria Sélia; Appoloni, Carlos Roberto; Parreira, Paulo Sérgio; Aragão, Pedro Henrique Arruda; Filho, V. F. Nascimento

doi:10.1590/s1516-89132007000500013

Cited by 11 publications

(11 citation statements)

References 8 publications

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“…For quantitative determination of elements, the emission–transmission (ET) method was chosen . An irradiator was prepared using a set of laboratory grade salts containing macronutrients (P, S, K, and Ca) and micronutrients (Mn, Fe, Cu, and Zn).…”

Section: Methodsmentioning

confidence: 99%

“…LIBS and XRF have been employed for the determination of macronutrients and micronutrients in different plant leaves, for example, sugarcane, cannabis , black tea, tomato, medicinal plants, orange, lemon, wheat, soybean, cotton, maize, and grass . However, in all the above mentioned studies, prior to analysis, the leaves were either detached from the plants or pelletized after washing, drying, and grinding …”

Section: Introductionmentioning

confidence: 99%

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Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

et al. 2019

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A portable energy dispersive X‐ray fluorescence (XRF) spectrometer furnished with an Rh X‐ray tube was evaluated for the determination of macronutrients and micronutrients in soybean leaves (Glycine max L.). XRF instrumental parameters were optimized in a univariate way, and emission intensities were measured for 60 s and under vacuum for macronutrients, and during 180 s, under air, and 305 μm Al/25.4 μm Ti filter, for micronutrients. Fresh and dried leaves were irradiated, and it was possible to identify P, K, Ca, S, Mn, Fe, Cu, and Zn Kα emission lines. For comparative purpose, the samples were also microwave assisted, digested and analyzed by inductively coupled plasma optical emission spectrometry. In general, linear correlations between K, Ca, Mn, Fe, Cu, and Zn concentrations in the tested samples and the corresponding portable XRF (pXRF) intensities were obtained. The linear correlation coefficients (R2) ranged from 0.42 to 0.86. In addition, the detection limits were suitable for plant nutrient diagnosis. It is demonstrated that pXRF is a simple and powerful tool for analysis of plant materials.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

et al. 2019

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“…In this case, the self-attenuation of the XRF is low enough to be ignored ( Van Grieken and Markowicz, 1993 ; Duran et al, 2017 ). In case of “intermediate thickness,” i.e., the XRF intensity depends on the sample thickness, the matrix effects can be calculated and corrected ( Van Grieken and Markowicz, 1993 ; Blonski et al, 2007 ). These two situations will be treated in detail below.…”

Section: Introductionmentioning

confidence: 99%

Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies

et al. 2018

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In vivo and micro chemical analytical methods have the potential to improve our understanding of plant metabolism and development. Benchtop microprobe X-ray fluorescence spectroscopy (μ-XRF) presents a huge potential for facing this challenge. Excitation beams of 30 μm and 1 mm in diameter were employed to address questions in seed technology, phytopathology, plant physiology, and bioremediation. Different elements were analyzed in several situations of agronomic interest: (i) Examples of μ-XRF yielding quantitative maps that reveal the spatial distribution of zinc in common beans (Phaseolus vulgaris) primed seeds. (ii) Chemical images daily recorded at a soybean leaf (Glycine max) infected by anthracnose showed that phosphorus, sulfur, and calcium trended to concentrate in the disease spot. (iii) In vivo measurements at the stem of P. vulgaris showed that under root exposure, manganese is absorbed and transported nearly 10-fold faster than iron. (iv) Quantitative maps showed that the lead distribution in a leaf of Eucalyptus hybrid was not homogenous, this element accumulated mainly in the leaf border and midrib, the lead hotspots reached up to 13,400 mg lead kg-1 fresh tissue weight. These case studies highlight the ability of μ-XRF in performing qualitative and quantitative elemental analysis of fresh and living plant tissues. Thus, it can probe dynamic biological phenomena non-destructively and in real time.

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“…For quantitative determination of elements, the ET method was chosen (BLONSKI et al, 2007;LEROUX;MAHMUD, 1966). An irradiator was prepared using a set of P.A.…”

Section: Emission-transmission (Et) Methodsmentioning

confidence: 99%

“…LIBS (GUERRA et al, 2015;CARVALHO et al, 2015b;AWASTHI et al, 2017;EL-DEFTAR et al, 2015;GONDAL et al, 2016) and XRF (GUERRA et al, 2018;ABEROUMAND, 2009;BLONSKI et al, 2007;CHUPARINA;AISUEVA, 2011;DALY;FENELON, 2018;DEMIR et al, 2010;KHRAMOVA et al, 2012;KHUDER et al, 2009;MCLAREN;GUPPY;TIGHE, 2012;NAYAK et al, 2011) have been employed for the determination of macro and micronutrients in different plant leaves, e.g., sugarcane (GUERRA et al, 2015;CARVALHO et al, 2015b;MELQUIADES et al, 2012), cannabis (EL-DEFTAR et al, 2015), black tea (GONDAL et al, 2016), tomato (CARVALHO et al, 2015b;DEMIR et al, 2010), medicinal plants (ABEROUMAND, 2009;CHUPARINA;AISUEVA, 2011;KHUDER et al, 2009), orange (BLONSKI et al, 2007), lemon (BLONSKI et al, 2007) FENELON, 2018). However, in all the above-mentioned studies, prior to analysis, the leaves were either detached from the plants (GUERRA et al, 2018) or pelletized after washing, drying and grinding (ABEROUMAND, 2009;BLONSKI et al, 2007;CHUPARINA;AISUEVA, 2011;DEMIR et al, 2010;KHRAMOVA ...…”

Section: Introductionmentioning

confidence: 99%

Foliar uptake of CuO and CeO2 nanoparticles by soybean (Glycine max L.)

Junior¹,

Tadeu²

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Nanotechnology presents a huge potential for plant nutrition. In this sense, foliar application of fertilizers has the advantages of delivering nutrients, reducing the environmental impact in relation to soil application. This study has investigated the effects of foliar application of CuO and CeO2 nanoparticles on soybean plants. Copper was chosen due to its importance as a nutrient for higher plants and all the processes the element participates. Cerium is the most abundant rare earth element in the Earth's crust and its abundance is not that different compared with Zn and Cu, which are much more involved in environmental investigations than Ce.During the study, it was attempted to develop an X-ray spectrometric method to trace the uptake and redistribution of nanoparticles in living plants. Even tough this was not possible, such method allowed to access the nutrional status of soybean under vivo conditions. The deposition of copper sources on leaves, such as nano and micro CuO and copper sulphate, caused damages, inducing to absence or scarce presence of platelets crystals. Shoot dry mass of plants was positively affected by the treatments, especially by the 40 nm nCuO. Copper speciation showed that the element undergoes redox reactions from Cu 1+ to Cu 2+ after 14 days of application. The nanoparticles were able to supply Cu and Ce to soybean since these elements were found in other plant tissues that were not directly exposed to the nanoparticles. However, it is not clear whether the particles are dissolved outside the leaves or taken up entirely. The use of cerium nanoparticles did not interfere in agronomic parameters such as biomass production and number of pods.

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Elementary chemical analysis in leaves infected by fumagina by X-Ray fluorescence technique

Abstract: Energy Dispersion X-Ray Fluorescence Technique (EDXRF) was employed to study the effects of the fumagina disease on the elementary chemical composition of the leaves. The experimental set up consisted of a Mo X-ray tube (K

Cited by 11 publications

References 8 publications

Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies

Foliar uptake of CuO and CeO2 nanoparticles by soybean (Glycine max L.)

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