Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin

Xu, Jinqiong; Qiao, Xiujuan; Yuan, Wei; Sheng, Qinglin; Yue, Tianli; Zheng, Jianbin; Zhou, Ming

doi:10.1007/s00604-019-3339-3

Cited by 71 publications

(27 citation statements)

References 32 publications

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“…In aptasensors, redox probe is charged oppositely to aptamer so that electrostatic repulsion makes the interface very sensitive to the shielding of aptamer charge or its compensation dye to target interactions. Such impedimetric sensors were reported for determination of other low‐molecular analytes, e. g., patulin , ochratoxin A , kanamycin , ibuprofen etc. The use of conductive polymers like PANI or poly( p ‐phenylene diamine) increases sensitivity of the detection due to pH‐ or charge dependency of underlying layer.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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Mycotoxins are highly toxic metabolites of some fungi that frequently contaminate water, food and feed and hence cause several human and animal diseases. In this work, a new approach to the fast and reliable determination of aflatoxin M1 (AFM1) in water and milk has been proposed with reagent free protocol of signal measurement. For this purpose, DNA aptamer selective to AFM1 was entrapped between two thin layers of polyaniline (PANI) electrodeposited on glassy carbon electrode. The incubation of the aptasensor in the AFM1 solution results in remarkable decrease of the PANI intrinsic activity monitored by direct current voltammetry or electrochemical impedance spectroscopy. Appropriate calibration curves were linear in the range from 3 to 90 ng/L with limit of detection (LOD) 1–5 ng/L depending on the measurement mode. Mechanism of signal generation involves shielding electrostatic interactions between the PANI and aptamer in the surface layer and variation of its redox activity attributed to the emeraldine form of PANI. Selectivity of the response was proved by similar experiments with aflatoxin B1 and ochratoxin A and by comparison of the results with those obtained with non‐specific aptamer in the sensing layer. Simple protocol for milk pretreatment has been proposed for reliable detection of AFM1 on the level of its threshold limited values (20 ng/L).

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Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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Section: Introductionmentioning

confidence: 99%

“…Molecularly imprinted polymers (MIPs) are functional receptors with specific rebinding sites for targets . Due to MIPs′ predetermined selectivity and high physical/thermal stability, they have received extensive attention in SPE , biosensors and chemosensors , biomimetic catalysis , and drug delivery . However, the reported MIPs are mainly synthesized in aprotic and low‐polar organic solvents , and their molecular recognition ability was very poor in aqueous solutions because of the disturbance of water molecules, which leads to nonspecific adsorption and restricts the practical applications .…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, in some cases, the nonspecific adsorption of MIPs in aqueous matrices is similar to or higher than the specific binding, which makes them unsuitable for recognizing targets in real samples . The preparation of MIPs mainly depends on the copolymerization of functional monomers with imprinted template, and the removing of template molecule can generate recognition sites with complementary sizes, shapes, and functionalities to the imprinted molecule . Therefore, the key to obtaining hydrophilic MIPs with excellent properties in water is to choose suitable functional monomers.…”

Section: Introductionmentioning

confidence: 99%

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One‐step polymerization of hydrophilic ionic liquid imprinted polymer in water for selective separation and detection of levofloxacin from environmental matrices

Zhu

Wang

et al. 2019

J of Separation Science

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A novel green hydrophilic levofloxacin imprinted polymer was presented via onestep polymerization in water using ionic liquid 1,6-hexa-3,3 ′ -bis-1-vinylimidazolium bromine with multiple hydrophilic groups and 2-hydroxyethyl methacrylate as a cofunctional monomer. Adsorption experiment revealed that the ionic liquid significantly improved the water compatible of imprinted polymer, and the excellent recognition of molecularly imprinted polymer for levofloxacin in water corresponds to the synergetic effect of H-bonding and the electrostatic and π-π interactions between the levofloxacin and co-functional monomer. Furthermore, the adsorption process of the imprinted material towards levofloxacin fitted the Langmuir model, and the maximum binding amount of levofloxacin onto the imprinted and corresponding non-imprinted polymer were 16.45 and 6.82 mg/g at 25 • C, respectively. After optimizing the parameters affecting solid phase extraction performance, an enrichment and determination system was achieved to separate and detect levofloxacin from water and sediment samples with recoveries that ranged from 83.67 to 101.33% and relative standard deviation of less than 5.59%. K E Y W O R D Sionic liquids, levofloxacin, molecularly imprinted polymers, water

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“…The modified gold electrode has a narrow linear response of 0.5 μg L −1 to 50 μg L −1 and a detection limit of 0.27 ng L −1 . Xu et al reported a voltammetric method developed with Ag nanoparticle/Zn-based metal organic framework (MOF) nanocomposite [17]. The method has a detection limit of 4.64 μg L −1 with a wide linear range of 1.55 μg L −1 to 1.55 × 10 5 μg L −1 .…”

Section: Introductionmentioning

confidence: 99%

Immunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles

2019

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A fluorometric immunoassay is described for the determination of patulin, a highly toxic fungal metabolite. A rabbit anti-patulin-bovine serum albumin (BSA) IgG conjugate was prepared and used to compose immunoliposomes and immunomagnetic nanoparticles. The immunomagnetic nanoparticles are then added to the sample to form the patulin-antibody composites which can be magnetically separated. The immunoliposomes are then added to form a sandwich. After magnetic separation of the composites and adding n-octyl-β-D-glucopyranoside, the fluorophore sulforhodamine B (SRB) is released. Its fluorescence intensity was then measured at excitation/emission wavelengths of 550/585 nm. The immunoliposome-based immunomagnetic nanoparticle assay can detect 8 μg L−1 of patulin in apple juice without the need for extraction, separation, and purification. The detection limit falls within the European regulatory limit for infants and children’s products (10 μg L−1). The method is rapid, enviroment-friendly, and reliable. Graphical abstractSchematic of the immunoassay.An immunoliposome-patulin-immunomagnetic nanoparticle sandwich complex is formed that can be separated from the sample by magnetic force. The fluorescence of sulforhodamine B carried inside of immunoliposome was measured to determine patulin. The method can detect 8 μg L−1 of patulin in apple juice. Electronic supplementary materialThe online version of this article (10.1007/s00604-019-3973-9) contains supplementary material, which is available to authorized users.

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Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin

Cited by 71 publications

References 32 publications

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

One‐step polymerization of hydrophilic ionic liquid imprinted polymer in water for selective separation and detection of levofloxacin from environmental matrices

Immunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles

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