Electrospun Water-Soluble Carboxyethyl Chitosan/Poly(vinyl alcohol) Nanofibrous Membrane as Potential Wound Dressing for Skin Regeneration

Abstract

Biocompatible carboxyethyl chitosan/poly(vinyl alcohol) (CECS/PVA) nanofibers were successfully prepared by electrospinning of aqueous CECS/PVA solution. The composite nanofibrous membranes were subjected to detailed analysis by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and X-ray diffraction (XRD). SEM images showed that the morphology and diameter of the nanofibers were mainly affected by the weight ratio of CECS/PVA. XRD and DSC demonstrated that there was strong intermolecular hydrogen bonding between the molecules of CECS and PVA. The crystalline microstructure of the electrospun fibers was not well developed. The potential use of the CECS/PVA electrospun fiber mats as scaffolding materials for skin regeneration was evaluated in vitro using mouse fibroblasts (L929) as reference cell lines. Indirect cytotoxicity assessment of the fiber mats indicated that the CECS/PVA electrospun mat was nontoxic to the L929 cell. Cell culture results showed that fibrous mats were good in promoting the cell attachment and proliferation. This novel electrospun matrix would be used as potential wound dressing for skin regeneration.

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

“…This property is useful for wound healing applications where hydrophilic or water soluble polymers are of interest for promoting the absorption of exudates and the delivery of water soluble drugs. [45][46][47] The analysis of the mechanical properties of the SA-PEO and SA-PEO/LO nanofibers revealed that they were characterized by a tensile strength of (13±2) MPa and (9±1) MPa, and by an elongation at break of (2.8±0.4)% and (1.6±0.2)%, respectively. Hence, the nanofibrous mats are robust enough to be easily handled without breaking, and they are flexible enough to adapt to skin wounds.…”

Chemical cross-linking of chitosan/polyvinyl alcohol electrospun nanofibers

“…26 In another study cross-linking of electrospun water-soluble carbo- xyethyl chitosan/poly (vinyl alcohol) nanofibrous membrane towards wound dressings for skin regeneration was performed using a GA vapor. 27 Unlike the previous work, in the present study, the cross-linking was performed using a lower concentration of GA vapor (5 %). Despite using this low concentration, it was proved that the fabricated membranes' structure is stable in an aqueous solution.…”

Nanofibrous Structure of Chitosan for Biomedical Applications

“…Chitosan based nanofibers along with other natural polymer based nanofibers have recently attracted a great deal of attention to be used as wound dressing. Chitosan is most suitable for this application because it meets several requirements such as histocompatibility, biodegradability, lack of antigenicity and also promotes wound healing [89][90][91].…”