Electrospinning of Bovine Serum Albumin. Optimization and the Use for Production of Biosensors.

Kowalczyk, Tomasz; Nowicka, Aleksandra; Elbaum, Danek; Kowalewski, T. A.

doi:10.1021/bm800421s

Cited by 98 publications

(86 citation statements)

References 15 publications

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“…These are directly related to the concentration and molecular weight of the polymer, as well as the solvent used. The dielectric properties of the solution also play a key role (Kowalczyk et al, 2008;Mitchell and Sanders, 2006;Thompson et al, 2007). In this study we sought to maintain several key parameters of the spinning process, specifically the voltage, collector type and rotation speed, the distance between the needle and the collector, the solution feed rate, and the solution viscosity.…”

Section: Discussionmentioning

confidence: 99%

Development of an Ibuprofen‐releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration

Cantón

McKean

Charnley

et al. 2009

Biotech & Bioengineering

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Our aim was to develop a biodegradable fibrous dressing to act as a tissue guide for in situ wound repair while releasing Ibuprofen to reduce inflammation in wounds and reduce pain for patients on dressing changes. Dissolving the acid form of Ibuprofen (from 1% to 10% by weight) in the same solvent as 75% polylactide, 25% polyglycolide (PLGA) polymers gave uniformly loaded electrospun fibers which gave rapid release of drug within the first 8 h and then slower release over several days. Scaffolds with 10% Ibuprofen degraded within 6 days. The Ibuprofen released from these scaffolds significantly reduced the response of fibroblasts to major pro-inflammatory stimulators. Fibroblast attachment and proliferation on scaffolds was unaffected by the addition of 1-5% Ibuprofen. Scaffolds loaded with 10% Ibuprofen initially showed reduced cell attachment but this was restored by soaking scaffolds in media for 24 h. In summary, addition of Ibuprofen to electrospun biodegradable scaffolds can give acute protection of adjacent cells to inflammation while the scaffolds provide an open 3D fibrous network to which cells can attach and migrate. By 6 days, such scaffolds will have completely dissolved into the wound bed obviating any need for dressing removal.

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Section: Discussionmentioning

confidence: 99%

Development of an Ibuprofen‐releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration

Cantón

McKean

Charnley

et al. 2009

Biotech & Bioengineering

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“…The spinneret needle was positively biased with a high voltage power supply. A flat cooper grid (310 mm × 240 mm) with a small cage (75 mm × 80 mm × 50 mm) was used as ground electrode, as described in previous reports [16,17]. The voltage applied between the spinneret and the ground electrode was varied from 1 kV up to 30 kV.…”

Section: Methodsmentioning

confidence: 99%

“…Such fibers find applications in a variety of areas, including wound dressing [4], drug or gene delivery vehicles [19], biosensors [16], fuel cell membranes and electronics [15]. Recently electrospinning has been revitalized and successfully applied to the production of nanofibrous scaffolds for tissue-engineering processes [2], which constitute one of its most promising application.…”

Section: Motivationmentioning

confidence: 99%

Modeling Electrospinning of Nanofibers

Kowalewski

Barral

Kowalczyk

IUTAM Symposium on Modelling Nanomaterials and Nanosystems

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A fast discrete model for the simulations of thin charged jets produced during the electrospinning process is derived, based on an efficient implementation of the boundary element method for the computation of electrostatic interactions of the jet with itself and with the electrodes. Short-range electrostatic forces are evaluated with slender-body analytical approximations, whereas a hierarchical force evaluation algorithm is used for long-range interactions. Qualitative comparisons with experiments is discussed. MotivationElectrospinning is a simple and relatively inexpensive mean of producing nanofibers by solidification of a polymer solution stretched by an electric field. Such fibers find applications in a variety of areas, including wound dressing [4], drug or gene delivery vehicles [19], biosensors [16], fuel cell membranes and electronics [15]. Recently electrospinning has been revitalized and successfully applied to the production of nanofibrous scaffolds for tissue-engineering processes [2], which constitute one of its most promising application.A conventional electrospinning setup consists of a spinneret with a metallic needle, a syringe pump, a high-voltage power supply and a grounded collector. A polymer solution is loaded into the syringe and driven through the needle at a steady and controllable feed rate by the pump, forming a droplet at the tip of the needle. A high voltage (typically up to 30 kV) is applied between the tip and a grounded collector. When the electric field strength overcomes the surface tension of the droplet an electrified liquid jet is formed. The jet is then elongated and whipped continuously by electrostatic repulsion (bending instability), describing a chaotic spiraling motion on its way to the collecting electrode. Although the process may appear

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“…The electrospinning of biopolymers such as chitosan polysaccharides [5][6][7][8][9][10], cellulose compounds [11][12][13][14], and proteins like collagen [15][16][17][18], zein [19][20][21][22][23][24], bovine serum albumin [25] and others [25][26][27][28][29][30] is well described in the literature. Electrospun polysaccharides [31,32] and other bio-polymer based nanofibres show significant potential for a variety of biomedical applications [33][34][35] such as tissue engineering, wound dressing and cosmetics [15,34,36,37].…”

Section: Introductionmentioning

confidence: 99%

Sub-micron sized saccharide fibres via electrospinning

Lepe¹,

Tucker²,

Simmons³

et al. 2015

Electrospinning

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Abstract:In this work, the production of continuous submicron diameter saccharide fibres is shown to be possible using the electrospinning process. The mechanism for the formation of electrospun polymer fibres is usually attributed to the physical entanglement of long molecular chains. The ability to electrospin continuous fibre from a low molecular weight saccharides was an unexpected phenomenon. The formation of sub-micron diameter "sugar syrup" fibres was observed in situ using highspeed video. The trajectory of the electrospun saccharide fibre was observed to follow that typical of electrospun polymers. Based on initial food grade glucose syrup tests, various solutions based on combinations of syrup components, i.e. mono-, di-and tri-saccharides, were investigated to map out materials and electrospinning conditions that would lead to the formation of fibre. This work demonstrated that sucrose exhibits the highest propensity for fibre formation during electrospinning amongst the various types of saccharide solutions studied. The possibility of electrospinning low molecular weight saccharides into sub-micron fibres has implications for the electrospinability of supramolecular polymers and other biomaterials.

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Electrospinning of Bovine Serum Albumin. Optimization and the Use for Production of Biosensors.

Cited by 98 publications

References 15 publications

Development of an Ibuprofen‐releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration

Development of an Ibuprofen‐releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration

Modeling Electrospinning of Nanofibers

Sub-micron sized saccharide fibres via electrospinning

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