Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses

Pliquett, Uwe; Elez, Robert; Piiper, Albrecht; Neumann, Eberhard

doi:10.1016/j.bioelechem.2003.11.001

Cited by 32 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recording of current and voltage during the electroporation pulse application is performed with a strategy similar to that described in Refs. [15,21,22]. Specific to our apparatus is the use of a small sensing resistor (1 Ω) for measurements instead of the use of a magnetic based current sensor.…”

Section: Apparatusmentioning

confidence: 99%

In vivo electrical impedance measurements during and after electroporation of rat liver

Ivorra

Rubinsky

2007

Bioelectrochemistry

154

View full text Add to dashboard Cite

Section: Apparatusmentioning

confidence: 99%

In vivo electrical impedance measurements during and after electroporation of rat liver

Ivorra

Rubinsky

2007

Bioelectrochemistry

154

View full text Add to dashboard Cite

“…After the electroporation pulse the impedance of the tissue between the electrodes is measured by using the fourelectrode method in which the electroporation electrodes are used as the current injection electrodes and a pair of smaller inner electrodes are used to measure the induced voltage difference across the tissue sample. Recording of current and voltage during the electroporation pulse application is performed with a strategy similar to that described in [10,13,14]. Specific to our apparatus is the use of a small sensing resistor (1 ) for measurements instead of the use of a magnetic based current sensor.…”

Section: A General Architecturementioning

confidence: 99%

Impedance Analyzer for in vivo Electroporation Studies

Ivorra¹,

Rubinsky²

2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

View full text Add to dashboard Cite

Electroporation, the permeabilization of the cell membrane with electrical pulses, is being used for in vivo gene therapy, drug therapy and minimally invasive tissue ablation. Applying electrical pulses across cells can have a variety of outcomes; from no effect to reversible electroporation to irreversible electroporation. For reliable in vivo use of electroporation it is important to have real time feedback on the outcome of the application of the electrical pulse. Recently, it has been proposed that measuring the electrical properties of electroporated tissues in temporal relation to the applied pulses could provide this feedback. To generate fundamental data on the in vivo electrical properties of electroporated tissues we have developed a fast spectroscopic impedance analyzer that measures electrical properties of tissues in vivo in conjunction with commercial electroporation pulse generators. Here we describe the apparatus and illustrate its use with an experiment on reversible electroporation in a rat liver.

show abstract

“…A set of possible methods for estimating electroporation could be based on measurements of the electrical properties of the tissues or cells electroporated [1], [5], [8], [12]- [15]. These electrical properties have been proposed to verify the electroporation effectiveness in individual cells [16], [17], cell suspensions [5], [8], [18], and tissues [14], [19].…”

Section: Introductionmentioning

confidence: 99%

Sinusoidal Signal Analysis of Electroporation in Biological Cells

Ramos

Schneider

Suzuki

et al. 2012

IEEE Trans. Biomed. Eng.

View full text Add to dashboard Cite

Conductivity measurements in suspensions of biological cells have been used since many years for electroporation effectiveness evaluation. However, conductivity modeling by means of instantaneous values of current and voltage during pulse application does not take into account the effects of the sample reactance and the dielectric dispersion of the medium. This can lead to misinterpretation in the electroporation analysis. The electrical modeling and characterization of electroporation using sinusoidal signal analysis at 10 kHz proposed in this paper allows us to avoid distortions due to reactive effects of the sample. A simple equation establishes the relation between suspension conductivity and membrane conductance. This model was used in experiments with suspensions of yeast cells and applied electric fields of up to 450 kV/m for 1 ms. The analysis using the proposed model resulted in membrane conductance values of up to 8000 S/m (2) and allowed estimating the distribution profile of conductance on the cell membrane.

show abstract

Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses

Cited by 32 publications

References 23 publications

In vivo electrical impedance measurements during and after electroporation of rat liver

In vivo electrical impedance measurements during and after electroporation of rat liver

Impedance Analyzer for in vivo Electroporation Studies

Sinusoidal Signal Analysis of Electroporation in Biological Cells

Contact Info

Product

Resources

About