Sinusoidal Signal Analysis of Electroporation in Biological Cells

Ramos, Alexandre Moraes; Schneider, A.; Suzuki, Daniela Ota Hisayasu; Marques, Jefferson Luiz Brum

doi:10.1109/tbme.2012.2212896

Cited by 10 publications

(8 citation statements)

References 37 publications

(89 reference statements)

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“…In this way, differences in the results were independent from the cell density but due to the different conditions tested. In fact, considering a diameter of 25 to 30 mm for MG-63 cells, 55 the volume fraction of cells 56,57 in monolayer is 0.52 to 0.75 that gives an effective electric field that is 84% to 95% of the applied one. From these data it was determined that by applying 1000 V/ cm the transmembrane voltage is 3.15 to 8.56 V. This transmembrane voltage is larger with respect to the typical one to form cell membrane pores 58 (ie, 0.2-1 V).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy

Ongaro

Campana

Mattei

et al. 2015

Technol Cancer Res Treat

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Electrochemotherapy (ECT) is a local anticancer treatment based on the combination of chemotherapy and short, tumorpermeabilizing, voltage pulses delivered using needle electrodes or plate electrodes. The application of ECT to large skin surface tumors is time consuming due to technical limitations of currently available voltage applicators. The availability of large pulse applicators with few and more spaced needle electrodes could be useful in the clinic, since they could allow managing large and spread tumors while limiting the duration and the invasiveness of the procedure. In this article, a grid electrode with 2-cm spaced needles has been studied by means of numerical models. The electroporation efficiency has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. The computational results show the distribution of the electric field in a model of the treated tissue. These results are helpful to evaluate the effect of the needle distance on the electric field distribution. Furthermore, the in vitro tests showed that the grid electrode proposed is suitable to electropore, by a single application, a cell culture covering an area of 55 cm 2 . In conclusion, our data might represent substantial improvement in ECT in order to achieve a more homogeneous and time-saving treatment, with benefits for patients with cancer.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy

Ongaro

Campana

Mattei

et al. 2015

Technol Cancer Res Treat

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“…The numerical simulations that predict or confirm these experimental findings are an important tool for the treatment planning of electroporation. Numerous studies have shown that the cell suspension conductivity is increased due to membrane electropermeabilization . The local electric field distribution within the treated tissue also produces conductivity increase .…”

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confidence: 99%

Numerical Model of Dog Mast Cell Tumor Treated by Electrochemotherapy

et al. 2014

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Electrochemotherapy is a combination of high electric field and anticancer drugs. The treatment basis is electroporation or electropermeabilization of the cell membrane. Electroporation is a threshold phenomenon and, for efficient treatment, an adequate local distribution of electric field within the treated tissue is important. When this local electric field is not enough, there is a regrown tumor cell; however, if it is stronger than necessary, permanent damage to the tissue occurs. In the treatment of dogs, electrochemotherapy is not yet an established treatment for mast cell tumor in veterinary medicine, although there are studies showing evidence of its effectiveness. In this study, we examined electrochemotherapy of dog mast cell tumor with numerical simulation of local electric field distribution. The experimental result was used to validate the numerical models. The effect of tumor position and tissue thickness (tumor in different parts of dog body) was investigated using plate electrodes. Our results demonstrated that the electrochemotherapy is efficient and flexible, and even when the tumor extends into the subcutis, the treatment with plate electrode eliminated the tumor cells. This result suggests that electrochemotherapy is a suitable method to treat mast cell tumors in dog.

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“…New insight was obtained into the kinetics of diffusion due to the creation of new interfaces at low frequency, which leads to a current path increase, i.e., decreased conductivity even with the presence of membrane pores. M A N U S C R I P T 13 1,209×10 13 4,643×10 13 3,155×10 14 7,736×10 13 A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 28 Highlights -An experimental protocol was designed to elucidate pulsed electric field effects on the electrode/electrolyte interface. -An electric field affects electrodes and the degree of alteration depends on the electrolyte conductivity.…”

Section: Discussionmentioning

confidence: 99%

“…Electrode/electrolyte interface impedance has strong dispersion of resistance and reactance at low frequencies. Ion diffusion within a double layer of cells also results in low frequency dielectric dispersion [13,14]. On the other hand, when a high-voltage electric pulse is applied to an electrolyte solution, a variety of electrolysis reactions occur at the electrode-solution interfaces in addition to cell membrane permeabilization [15].…”

Section: Introductionmentioning

confidence: 99%