In vivo electrical impedance measurements during and after electroporation of rat liver

Ivorra, Antoni; Rubinsky, Boris

doi:10.1016/j.bioelechem.2006.10.005

Cited by 154 publications

(115 citation statements)

References 28 publications

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“…However, the rapid resealing of electroporated membranes prohibits a detailed frequency sweep; thus in practice, only one point or a few points of the spectrum are measured, typically in the kilohertz range. Successful implementation of this approach has been reported, e.g., for skin [57] and liver [58], allowing for a distinction between nonporated, reversibly porated, and irreversibly porated tissues.…”

Section: Monitoring Of Electroporationmentioning

confidence: 99%

Cell membrane electroporation- Part 1: The phenomenon

Kotnik

Kramar

Pucihar

et al. 2012

IEEE Electr. Insul. Mag.

407

212

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Section: Monitoring Of Electroporationmentioning

confidence: 99%

Cell membrane electroporation- Part 1: The phenomenon

Kotnik

Kramar

Pucihar

et al. 2012

IEEE Electr. Insul. Mag.

407

212

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“…If the relative conductivity around the electrodes is increased by the IRE, the region of high electric field comes to spread farther and farther, resulting in a gradual expansion of the necrotic cell region. The change in electrical properties was addressed by previous studies [37,38]. Ivorra et al showed that 10 to 180 % increase of electrical conductivity was observed depending on the applied electroporation protocol after the treatment of mice fibrosarcomas [37].…”

Section: Discussionmentioning

confidence: 98%

Experimental and Analytical Studies on Contact Irreversible Electroporation for Superficial Tumor Treatment

Kurata

Ueno

Matsushita

et al. 2013

JBSE

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Irreversible electroporation (IRE) is attracting much attention as a less-invasive therapy to ablate abnormal tissues. With a pair of electrodes inserted into the tissue, the IRE perforates the targeted cells non-thermally by a train of intensive electric pulses that exceeds a certain threshold. Since only the cells are necrotized percutaneously, the extracellular matrix is kept intact in the IRE, which is favorable for prompt tissue regeneration. In this study, we demonstrated that the IRE is also applicable to treat superficial targets such as melanoma, nevus, and tumors on gastrointestinal surface. A pair of 1-mm dia. stainless steel rods fixed 5 mm apart in an acrylic plate was contacted on an agarose gel containing fibroblasts. A sequence of 15 to 90 pulses of 1 kV was then applied to the gel via electrodes. All pulses were 10 µs in length and oscillated at 100 ms intervals. The boundary between dead and alive cell regions was determined at the surface and vertical cross section by using fluorescent staining. The necrotic cell area was also predicted by a numerical solution to the equation of electric field. Those determined with an assumption that the potential difference of 1 V between the cell membrane induces irreversible cell breakdown agreed well with the necrotic cell area and maximal depth in the experiment at low pulse number. However, the experiment showed the increase in the necrotic area with increasing the pulse repetition. Since application of multiple pulses perforates the highly resistive cell membrane, our study indicated the importance of taking into account the change in the electrical properties due to cell destruction.

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“…1) to model the increase in tissue electrical conductivity during pulse delivery due to EP [15]. It has recently been shown that modeling this effect significantly improves the accuracy of these simulations [16,17].…”

Section: Theoretical Model: Ep Problemmentioning

confidence: 99%

“…It has recently been shown that modeling this effect significantly improves the accuracy of these simulations [16,17]. DC conductivity at low electric field magnitudes (σ 0 = 0.08 S/m) and DC conductivity at very high fields (σ MAX = 0.31 S/m = σ 0 + Δσ) were obtained from [15]. The sigmoid behavior and the parameters that determine its shape were adjusted from data in [18].…”

Section: Theoretical Model: Ep Problemmentioning

confidence: 99%

Can electroporation previous to radiofrequency hepatic ablation enlarge thermal lesion size? A feasibility study based on theoretical modelling andin vivoexperiments

Trujillo

Castellví

Burdı́o

et al. 2013

International Journal of Hyperthermia

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Castellvi, Q.; Burdío, F.; Sánchez Velázquez, P.; Ivorra, A.; Andaluz, A.; Berjano, E. (2013). Can electroporation previous to radiofrequency hepatic ablation enlarge thermal lesion size? A feasibility study based on theoretical modelling and in vivo experiments. AbstractPurpose: To assess the feasibility of a hybrid ablative technique based on applying electroporation (EP) pulses just before conducting radiofrequency ablation (RFA). The rationale was that the EP-induced reduction in blood perfusion could be sufficient to reduce the thermal sink effect and hence to increase the coagulation volume in comparison to that created exclusively by RFA. Materials and methods:Modeling study and in vivo experimental study. A Cool-tip RF applicator was used both for EP and RFA.Results: Overall, the results did not show any synergy effect from using the hybrid technique. Applying EP pulses prior to RFA did not increase the coagulation zone obtained and the lesions were almost identical. Additional computer simulations provided an explanation for this; the effect of reducing blood perfusion by thermal damage during RFA completely masks the effect of reducing blood perfusion by EP. This is because both thermal damage and EP affect the same zone, i.e. the tissue around the electrode. Conclusions:Our computer modeling and in vivo experimental findings suggest that the combination of EP and RFA with monopolar applicators does not provide an additional benefit over the use of RFA alone.

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In vivo electrical impedance measurements during and after electroporation of rat liver

Cited by 154 publications

References 28 publications

Cell membrane electroporation- Part 1: The phenomenon

Cell membrane electroporation- Part 1: The phenomenon

Experimental and Analytical Studies on Contact Irreversible Electroporation for Superficial Tumor Treatment

Can electroporation previous to radiofrequency hepatic ablation enlarge thermal lesion size? A feasibility study based on theoretical modelling andin vivoexperiments

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