Cell membrane electroporation- Part 1: The phenomenon

Kotnik, Tadej; Kramar, Peter; Pucihar, Gorazd; Miklavčič, Damijan; Tarek, Mounir

doi:10.1109/mei.2012.6268438

Cited by 390 publications

(208 citation statements)

References 67 publications

(70 reference statements)

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“…However, when the induced transmembrane voltage (ITV) is too high, pores form in the cell membrane, resulting in the process called electroporation. 34 The induced transmembrane voltage for a spherical cell in suspension can be described with the following equation: 35,36 …”

Section: Theorymentioning

confidence: 99%

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

et al. 2016

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We designed a new microfluidic device that uses pillars on the same order as the diameter of a cell (20 lm) to isolate and enrich rare cell samples from background. These cell-scale microstructures improve viability, trapping efficiency, and throughput while reducing pearl chaining. The area where cells trap on each pillar is small, such that only one or two cells trap while fluid flow carries away excess cells. We employed contactless dielectrophoresis in which a thin PDMS membrane separates the cell suspension from the electrodes, improving cell viability for off-chip collection and analysis. We compared viability and trapping efficiency of a highly aggressive Mouse Ovarian Surface Epithelial (MOSE) cell line in this 20 lm pillar device to measurements in an earlier device with the same layout but pillars of 100 lm diameter. We found that MOSE cells in the new device with 20 lm pillars had higher viability at 350 V RMS , 30 kHz, and 1.2 ml/h (control 77%, untrapped 71%, trapped 81%) than in the previous generation device (untrapped 47%, trapped 42%). The new device can trap up to 6 times more cells under the same conditions. Our new device can sort cells with a high flow rate of 2.2 ml/h and throughput of a few million cells per hour while maintaining a viable population of cells for off-chip analysis. By using the device to separate subpopulations of tumor cells while maintaining their viability at large sample sizes, this technology can be used in developing personalized treatments that target the most aggressive cancerous cells. V C 2016 AIP Publishing LLC. [http://dx

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Section: Theorymentioning

confidence: 99%

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

et al. 2016

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“…ECT is a novel treatment modality consisting in the use of an electric field to increase tissue permeability to certain drugs [8,9]. Bleomycin is usually the drug of choice, which works as an endonuclease, cutting the DNA strands and thus interfering with cell division.…”

Section: Introductionmentioning

confidence: 99%

Canine Oral Eosinophilic Granuloma Treated with Electrochemotherapy

Tellado

Michinski

Olaiz

et al. 2014

Case Reports in Veterinary Medicine

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A case of a canine oral eosinophilic granuloma in a 14-year-old female crossbred is described. The dog was presented with a history of ptyalism, halitosis, local pain, decreased appetite, and blood staining noted on food and water bowls. Clinical, hematologic, and biochemical examinations, abdominal ultrasonography, and 3-view chest radiographs were performed, and no metastases were found. Histopathologic examination of two 6 mm punch biopsies from the oral lesion revealed the presence of eosinophilic granulomatous lesions in the submucosa. After treatment with corticosteroids and wide spectrum antibiotics no significant changes in clinical signs and lesion size were observed. Electrochemotherapy (ECT), a novel tumor treatment routinely used for cutaneous and subcutaneous tumors in human patients in the European Union since 2006, was used to treat the eosinophilic granuloma. The procedure was performed under general anesthesia, followed by intravenous administration of bleomycin. Six weeks after treatment a complete response with disappearance of the mass and improvement of clinical signs were observed.

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“…The criterion of the ablation is that the local electric field strength exceeds a critical value. [23][24][25][26] Assuming the time-independent electrical conductivity of tissue, 8 this criterion results in a timeindependent ablation area. However, in an experiment, the ablated area increases as the time of electric shock increases, and this has been empirically modeled by a reduction in the critical electric field over time.…”

Section: Analytic Modeling Of the Ablation Areamentioning

confidence: 99%

Histological and Mathematical Analysis of the Irreversibly Electroporated Liver Tissue

Sung

Kim

Jung

et al. 2016

Technol Cancer Res Treat

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Irreversible electroporation has clinically been used to treat various types of cancer. A plan on how to apply irreversible electroporation before practicing is very important to increase the ablation area and reduce the side effects. Several electrical models have been developed to predict the ablation area with applied electric energy. In this experiment, the static relationship between applied electric energy and ablated area was mathematically and experimentally investigated at 10 hours after applying irreversible electroporation. We performed the irreversible electroporation on the liver tissue of Sprague Dawley rats (male, 8 weeks, weighing 250-350 g). The ablated area was measured based on histological analysis and compared with the mathematical calculation from the electric energy, assuming that the tissue is homogeneous. The ablated area increased with the increase in applied electric energy. The numerically calculated contour lines of electric energy density overlapped well with the apoptotic area induced by the irreversible electroporation. The overlapped area clearly showed that the destructive threshold of apoptosis between electrodes is electric energy density level of 5.9 Â 10 5 J/m 3 . The results of the present study suggested that the clinical results of the irreversible electroporation on a liver tissue could be predicted through mathematical calculation.

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Cell membrane electroporation- Part 1: The phenomenon

Cited by 390 publications

References 67 publications

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures

Canine Oral Eosinophilic Granuloma Treated with Electrochemotherapy

Histological and Mathematical Analysis of the Irreversibly Electroporated Liver Tissue

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