Electrophoresis of Proteins in Agar Jelly

Ah, Gordon; Keil, B.; Šebesta, K.

doi:10.1038/164498a0

Cited by 54 publications

(5 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of hydrogels in electrophoresis can be traced back to 1949 from the reported literature. 14 The early form of electrophoresis usually involved a bulk of solution as background electrolyte (BGE). To cut down the BGE convection currents and diffusion, the agar gel was employed as a support media of the aqueous BGE, so that the BGE flow is stabilized and the separated protein remain as sharp zones.…”

Section: Applications Of Hydrogel In Electrophoresis 2•1 Support Mediamentioning

confidence: 99%

Hydrogels in Electrophoresis: Applications and Advances

2020

View full text Add to dashboard Cite

show abstract

Section: Applications Of Hydrogel In Electrophoresis 2•1 Support Mediamentioning

confidence: 99%

Hydrogels in Electrophoresis: Applications and Advances

2020

View full text Add to dashboard Cite

show abstract

“…The use of agar gel to suppress convection currents wdiich interfere with electrophoretic separations is gain-ing favor (1,5,6). Agar gel gives better fractionation and permits larger sample volumes than does filter paper; furthermore, the separated fractions are easier to locate than when opaque materials such as powdered glass (2) or starch (3) are used as supporting media.…”

Section: Literature Citedmentioning

confidence: 99%

Determination Serum Proteins Gravimetrically after Agar Electrophoresis

Strickland¹,

Mack²,

Gurule³

et al. 1959

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…With advantageous physicochemical properties, agarose is one of the two major components of a mixture called agar and extracted from red algae by boiling, filtration, and freeze-thawing to remove impurities and the other main component of agaropectin [ 1 , 2 ]. As early as in 1949, Gordon et al used agar jelly for protein electrophoresis to separate ferritin from hemoglobin and to resolve egg white proteins [ 3 ]. However, protein electrophoresis in unprocessed agar was most often compromised by adsorption of sample particles to, or precipitation in the gel [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins

Sarkozy

Guttman

2022

Gels

View full text Add to dashboard Cite

Capillary sodium dodecyl sulfate gel electrophoresis has long been used for the analysis of proteins, mostly either with entangled polymer networks or translationally cross-linked gels. In this paper capillary agarose gel electrophoresis is introduced for the separation of low molecular weight immunoglobulin subunits. The light (LC~24 kDa) and heavy (HC~50 kDa) chain fragments of a monoclonal antibody therapeutic drug were used to optimize the sieving matrix composition of the agarose/Tris-borate-EDTA (TBE) systems. The agarose and boric acid contents were systematically varied between 0.2–1.0% and 320–640 mM, respectively. The influence of several physical parameters such as viscosity and electroosmotic flow were also investigated, the latter to shed light on its effect on the electrokinetic injection bias. Three dimensional Ferguson plots were utilized to better understand the sieving performance of the various agarose/TBE ratio gels, especially relying on their slope (retardation coefficient, KR) value differences. The best resolution between the LC and non-glycosylated HC IgG subunits was obtained by utilizing the molecular sieving effect of the 1% agarose/320 mM boric acid composition (ΔKR = 0.035). On the other hand, the 0.8% agarose/640 mM boric acid gel showed the highest separation power between the similar molecular weight, but different surface charge density non-glycosylated HC and HC fragments (ΔKR = 0.005). It is important to note that the agarose-based gel-buffer systems did not require any capillary regeneration steps between runs other than simple replenishment of the sieving matrix, significantly speeding up analysis cycle time.

show abstract

Electrophoresis of Proteins in Agar Jelly

Cited by 54 publications

References 2 publications

Hydrogels in Electrophoresis: Applications and Advances

Hydrogels in Electrophoresis: Applications and Advances

Determination Serum Proteins Gravimetrically after Agar Electrophoresis

Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins

Contact Info

Product

Resources

About