Determination Serum Proteins Gravimetrically after Agar Electrophoresis

Abstract

Bromophenol blue Ponceau 2R Rho 2.57 ± 0.29 3.90 ± 0.03 3.54 ± 0.63 Albumin-1 2.69 ± 0.53 4.83 ± 0.19 2.54 ± 0.48 Albumin-2 3.24 ± 0.63 4.15 ± 0.18 4.27 ± 1.29 Alphai 3.47 ± 0.82 5.26 ± 0.69 4.81 ± 1.54 Alpha2-1 3.82 ± 0.88 4.96 ± 0.55 5.31 ±1.54

Chromatography

“…Zone detection with electrochromatography is not significantly different from that in chromatography (S3, 121, 126, 411), but recovery of separated substances from gels (136,383,404) mon pH buffers. Designed for use with biochemical substances, a number of these buffers are salts of strong bases and weak acids or of strong acids and weak bases (136, 404, 424)• These buffers show rapid change of pH at the electrodes when employed as background electrolytic solutions.…”

Dye-Binding Capacities of Eleven Electrophoretically Separated Serum Proteins

“…The use of agar gel to suppress convection currents wdiich interfere with electrophoretic separations is gain-ing favor (1,5,6). Agar gel gives better fractionation and permits larger sample volumes than does filter paper; furthermore, the separated fractions are easier to locate than when opaque materials such as powdered glass (2) or starch (3) are used as supporting media.…”