Electronically conductive polymer grafted with oligonucleotides as electrosensors of DNA

Lassalle, N.; Mailley, Pascal; Vieil, E.; Livache, Thierry; Roget, André; Correia, J. Pinto; Abrantes, L.M.

doi:10.1016/s0022-0728(01)00537-x

Cited by 90 publications

(62 citation statements)

References 31 publications

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“…Such a decrease in the photocurrent response was not observed for the spectral response of the copolymer since the electrode was rinsed with phosphate buffer prior to photocurrent measurement. The kinetics for the non complimentary target was k ¼ 1.7 Â 10 À3 s À1 (293 K) while for the hybridizes sample k ¼ 1.67 Â 10 À2 s À1 (293 K) [50]. Even though these results are preliminary they show the interest in using the kinetic as indication for complimentary or non complimentary hybridization.…”

Section: Photoelectrochemistrymentioning

confidence: 84%

“…Polypyrrole was not only used in this application as material for DNA immobilization but the polypyrrole matrix also acted as an integrated molecular transducer [13,41,42]. We also show that the photoelectrochemical properties of polypyrrole towards hybridization could be investigated by photocurrent spectroscopy, opening a different way of molecular transduction by the DNA host matrix [20].…”

mentioning

confidence: 80%

“…The strategy is based on a copolymerization process of pyrrole and oligonucleotides containing a pyrrole moiety on the 5' end, using phosphoramidite chemistry (1). [20] This electrocontrolled synthesis of the copolymer gives a solid conducting film deposited in the surface of the electrode in one step.…”

Section: Electropolymerizationmentioning

confidence: 99%

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Comparison of Different Strategies on DNA Chip Fabrication and DNA-Sensing: Optical and Electrochemical Approaches

et al. 2005

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International audienceNew strategies for the construction of DNA chips and the detection of DNA hybridization will be discussed in this review. The focus will be on the use of polypyrrole as a linker between a substrate and oligonucleotide probes. The modification step is based on the electrochemical copolymerization of pyrrole and oligonucleotides bearing a pyrrole group on its 5′ end. This strategy was employed for the immobilization of oligonucleotides on millimeter-sized electrodes, microelectrode arrays, as well as for the local structuring of homogeneous gold surfaces. Our approaches for the localized patterning of gold surfaces will be also discussed. Localized immobilization was achieved by using an electrospotting technique, where a micropipette served as an electrochemical cell where spot sizes with 800 μm diameters were fabricated. The use of a microcell using a Teflon covered metal needle with a cavity of 100 μm resulted in immobilized probe spots of 300 μm. Scanning electrochemical microscopy (SECM) was also used, and surface modifications of 100 μm were obtained depending on the experimental conditions. Different detection methods were employed for the reading of the hybridization event: fluorescence imaging, surface plasmon resonance imaging (SPRI), photocurrent measurements, and voltamperometric measurements using intercalators. Their advantages concerning the various immobilization strategies will also be discussed

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Section: Photoelectrochemistrymentioning

confidence: 84%

mentioning

confidence: 80%

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Comparison of Different Strategies on DNA Chip Fabrication and DNA-Sensing: Optical and Electrochemical Approaches

et al. 2005

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“…Published techniques include the use of a biotinylated probe coupled to an avidin or streptavidin layer Hook et al, 2001b;Tombelli et al, 2002;Gao et al, 2004;, an avidinmodified polymeric surface such as polypyrrole (DupontFilliard et al, 2001(DupontFilliard et al, , 2004b, polystyrene Su, 2002), polyallylamine , thioctic acid (Taira and Yokoyama, 2005), covalently linked polypyrrole-bearing oligonucleotides (Lassalle et al, 2001), thiolated probes bound directly to the surface (Hianik et al, 2001;Huang et al, 2001;Hook et al, 2001b;Ha et al, 2004;Mannelli et al, 2005), photoreactive biotin (Su, 2002), dextran Tombelli et al, 2005b), alkane-thiol mixed SAMs , amine labelled DNA covalently bound to the surface via a modified silane (Gheorghe and Guiseppi-Elie, 2003 Ihalainen and Peltonen, 2004), cholesterol-tagged DNA on lipid layers (Graneli et al, 2004;Pfeiffer and Hook, 2004), schizophyllan (a beta-(1,3)-D-glucan) complexed DNA and single-chain schizophyllan complexed with polynucleotides.…”

Section: Oligonucleotide Immobilizationmentioning

confidence: 99%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

335

205

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The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

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“…The immobilization of ODNs functionalized by one pyrrole group has been successfully carried out by electropolymerization or copolymerization with regular pyrrole [23,30,105,106]. Indeed, an electrochemical copolymer- ization process with unsubstituted monomers was often required for biopolymer formation.…”

Section: Electropolymerization Of Bioreceptorsmentioning

confidence: 99%

Affinity Biosensors Based on Electropolymerized Films

Cosnier

2005

Electroanalysis

147

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This review gives an overview on different types of affinity biosensors based on electropolymerized polymer films that are becoming an important class of analytical tools. These affinity biosensors may be classified according to the strategy used for their fabrication, namely entrapment within polymers during their electrochemical growth, simple adsorption onto electropolymerized films, chemical coupling or affinity interactions between bioreceptors and electropolymerized films or direct electrochemical polymerization of the bioreceptor itself. Recently opened perspectives and potential research directions are also discussed.

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Electronically conductive polymer grafted with oligonucleotides as electrosensors of DNA

Cited by 90 publications

References 31 publications

Comparison of Different Strategies on DNA Chip Fabrication and DNA-Sensing: Optical and Electrochemical Approaches

Comparison of Different Strategies on DNA Chip Fabrication and DNA-Sensing: Optical and Electrochemical Approaches

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Affinity Biosensors Based on Electropolymerized Films

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