Affinity Biosensors Based on Electropolymerized Films

Cosnier, Serge

doi:10.1002/elan.200503308

Cited by 147 publications

(92 citation statements)

References 124 publications

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“…One approach to electrode functionalization is to use polymer films to which biomolecular probes can be attached or entrapped [204]. Either faradaic or nonfaradaic measurement can subsequently be employed.…”

Section: Polymer Filmsmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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Section: Polymer Filmsmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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“…[1][2][3][4][5] Among this wide family of organic materials, electroactive polymers (EPs) have emerged as attractive candidates for sensing elements due to its unique electrochemical, electrical and optical properties.…”

Section: Introductionmentioning

confidence: 99%

Fmoc–RGDS based fibrils: atomistic details of their hierarchical assembly

Zanuy

Poater

Solà

et al. 2016

Phys. Chem. Chem. Phys.

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The interaction between morphine (MO), a very potent analgesic psychoactive drug, and five electroactive polymers, poly(3,4-ethylenedioxythiophene) (PEDOT), poly(3-methylthiophene) (P3MT), polypyrrole (PPy), poly(N-methylpyrrole (PNMPy) and poly[N-(2-cyanoethyl)pyrrole] (PNCPy), has been examined using theoretical calculations on model complexes and voltammetric measures considering different pHs and incubation times. Quantum mechanical calculations in model polymers predict that the strength of the binding between the difference polymers and morphine increases as follows: PEDOT < PNMPy < PPy << P3MT ≈ PNCPy. The most relevant characteristic of P3MT is its ability to interact with morphine exclusively through non-directional interactions. On the other hand, the variations of the electroactivity and the anodic current at the reversal potential evidence that the voltammetric response towards the presence of MO is considerably higher for P3MT and PNCPy than that for the other polymers at both acid (P3MT > PNMPy) and neutral (P3MT ≈ PNCPy) pHs. Energy decomposition analyses of the interaction of MO with different model polymers indicate that the stronger affinity of MO for P3MT and PNCPy as compared to PEDOT, PNMPy, and PPy is due to more favorable orbital interactions. These more stabilizing orbital interactions are the result of the larger charge transfer from MO to P3MT and PNCPy model polymers that takes place because of the higher stability of the single occupied molecular orbital (SOMO) of these model polymers. Therefore, to design polymers with a large capacity to detect MO we suggest looking at polymers with high electron affinity.3

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“…They highlighted the permeability, permselectivity and transmembrane potential of the polymer membranes and the role of polymer membranes as matrices for the immobilization of reactive chemical and biological agents. Cosnier reviewed the application of the electropolymerized films on the construction of affinity sensor [19]. He compared the different strategies for the immobilization of biomolecules on electropolymerized films to construct affinity sensors which can be used as clinical sensors.…”

Section: Electropolymerized Films For Clinical Monitoringmentioning

confidence: 99%