Electronic Cortisol Detection Using an Antibody-Embedded Polymer Coupled to a Field-Effect Transistor

Jang, Hyun‐June; Lee, Taein; Song, Jian; Russell, Luisa M.; Li, Hui; Dailey, Jennifer; Searson, Peter C.; Katz, Howard E.

doi:10.1021/acsami.7b18855

Cited by 67 publications

(57 citation statements)

References 28 publications

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“…In addition, the sensing performance can further improved by controlling the Debye length (λ D ). For example, Jang et al have described an antibody embedded polymer matrix configuration in FET sensors that allows the effective detection of charge variations between membrane-substrate interfaces [36]. Figure 4b shows the corresponding calibration curve of our cortisol FET sensor, which demonstrates a linear response.…”

Section: Real-time Response To Cortisolmentioning

confidence: 92%

Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor

et al. 2020

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Stress biomarkers such as hormones and neurotransmitters in bodily fluids can indicate an individual’s physical and mental state, as well as influence their quality of life and health. Thus, sensitive and rapid detection of stress biomarkers (e.g., cortisol) is important for management of various diseases with harmful symptoms, including post-traumatic stress disorder and depression. Here, we describe rapid and sensitive cortisol detection based on a conducting polymer (CP) nanotube (NT) field-effect transistor (FET) platform. The synthesized polypyrrole (PPy) NT was functionalized with the cortisol antibody immunoglobulin G (IgG) for the sensitive and specific detection of cortisol hormone. The anti-cortisol IgG was covalently attached to a basal plane of PPy NT through an amide bond between the carboxyl group of PPy NT and the amino group of anti-cortisol IgG. The resulting field-effect transistor-type biosensor was utilized to evaluate various cortisol concentrations. Cortisol was sensitively measured to a detection limit of 2.7 × 10−10 M (100 pg/mL), with a dynamic range of 2.7 × 10−10 to 10−7 M; it exhibited rapid responses (<5 s). We believe that our approach can serve as an alternative to time-consuming and labor-intensive health questionnaires; it can also be used for diagnosis of underlying stress-related disorders.

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Section: Real-time Response To Cortisolmentioning

confidence: 92%

Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor

et al. 2020

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“…The cortisol levels in various bodily fluids can range from 4 pM to 70 μM depending on the fluid. In sweat, the optimum level of cortisol ranges from 0.02 to 0.5 μM ( 18 , 19 ). Increased levels of cortisol have a detrimental effect on the regulation of various physiological processes such as blood pressure, glucose levels, and carbohydrate metabolism, and sustained stress can disrupt homeostasis in the cardiovascular, immune, renal, skeletal, and endocrine systems, leading to development of chronic diseases ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

Molecularly selective nanoporous membrane-based wearable organic electrochemical device for noninvasive cortisol sensing

et al. 2018

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“…For instance, λ D in phosphate-buffered saline (1× PBS), which is commonly used in biological research is <1 nm. The physical lengths of antibody–antigen complexes, usually utilized for ISFET biosensors, are larger than λ D associated with physiological media 35 . Therefore, the challenge for designing a FET sensor for detection of the cortisol is the choice of an appropriate catch probe overcoming the Debye length.…”

Section: Introductionmentioning

confidence: 99%

Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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Cortisol is a hormone released in response to stress and is a major glucocorticoid produced by adrenal glands. Here, we report a wearable sensory electronic chip using label-free detection, based on a platinum/graphene aptamer extended gate field effect transistor (EG-FET) for the recognition of cortisol in biological buffers within the Debye screening length. The device shows promising experimental features for real-time monitoring of the circadian rhythm of cortisol in human sweat. We report a hysteresis-free EG-FET with a voltage sensitivity of the order of 14 mV/decade and current sensitivity up to 80% over the four decades of cortisol concentration. The detection limit is 0.2 nM over a wide range, between 1 nM and 10 µM, of cortisol concentrations in physiological fluid, with negligible drift over time and high selectivity. The dynamic range fully covers those in human sweat. We propose a comprehensive analysis and a unified, predictive analytical mapping of current sensitivity in all regimes of operation.

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Electronic Cortisol Detection Using an Antibody-Embedded Polymer Coupled to a Field-Effect Transistor

Cited by 67 publications

References 28 publications

Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor

Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor

Molecularly selective nanoporous membrane-based wearable organic electrochemical device for noninvasive cortisol sensing

Extended gate field-effect-transistor for sensing cortisol stress hormone

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