Electron probe analysis and biochemical characterization of electron-dense granules secreted by Entamoeba histolytica

León, Gloria; Fiori, Charles E.; Das, Pradeep; Moreno, M.; Tovar, Rosalinda; Sánchez-Salas, José Luis; Muñoz, Marı́a de Lourdes

doi:10.1016/s0166-6851(97)02833-8

Cited by 19 publications

(9 citation statements)

References 30 publications

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“…In addition to a collagenolytic activity, EDGs seem to contain at least 25 polypeptides with acidic pIs (6.06 to 6.59), 9 gelatinase activities, actin, small molecules including inorganic phosphate (P i ) and pyrophosphate (PP i ), and several ions. Six of these polypeptides (108,106,104,97,68, and 59 kDa) and two protease activities of 40 and 85 kDa have been claimed to be detected in EDGs but not in total-trophozoite extracts (76). Whether some of the abovementioned components represent contamination with cytoplasmic molecules not found in EDGs remains to be established.…”

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 96%

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

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Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 96%

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

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“…Dense granules were described in different amoebas in early electron microscopical studies (Rondanelli et al 1965;Sbota 1985;Martínez-Palomo et al 1987), and several proteolytic activities have been related to them (León et al 1997). At the beginning of the excystation process, dense granules are present in the cytoplasm of E. invadens (Chávez-Munguía et al 2003).…”

Section: Excystationmentioning

confidence: 99%

Ultrastructure of cyst differentiation in parasitic protozoa

Chávez‐Munguía¹,

Omaña‐Molina²,

González‐Lázaro³

et al. 2007

Parasitol Res

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Cysts represent a phase in the life cycle of biphasic parasitic protozoa that allow them to survive under adverse environmental conditions. Two events are required for the morphological differentiation from trophozoite to cyst and from cyst to trophozoite: the encystation and excystation processes. In this paper, we present a review of the ultrastructure of the encystation and excystation processes in Entamoeba invadens, Acanthamoeba castellanii, and Giardia lamblia. The comparative electron microscopical observations of these events here reported provide a morphological background to better understand recent advances in the biochemistry and molecular biology of the differentiation phenomena in these microorganisms.

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“…The metallocollagenase of E. histolytica is a surface-bound proteinase and is expressed in lesser amounts than the thiol proteinase. It degrades type I and type III collagen, and its expression also correlates with pathogenicity of Entamoeba (52,103). The gene encoding the metallocollagenase of Entamoeba has not yet been identified.…”

Section: Multiple Forms Of Cysteine Proteinasesmentioning

confidence: 99%