Ultrastructure of cyst differentiation in parasitic protozoa

Chávez‐Munguía, Bibiana; Omaña‐Molina, Maritza; González‐Lázaro, Mónica; González‐Robles, Arturo; Cedillo‐Rivera, Roberto; Bonilla, Patricia; Martı́nez-Palomo, Adolfo

doi:10.1007/s00436-006-0447-x

Cited by 41 publications

(29 citation statements)

References 44 publications

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“…Encystation and excystation are two major differentiation steps that are crucial for the completion of the Entamoeba life cycle [27]. The major components of the Entamoeba cyst wall are chitin and chitosan [4].…”

Section: Discussionmentioning

confidence: 99%

The chitin biosynthesis pathway in Entamoeba and the role of glucosamine-6-P isomerase by RNA interference

Samanta

Ghosh

2012

Molecular and Biochemical Parasitology

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Section: Discussionmentioning

confidence: 99%

The chitin biosynthesis pathway in Entamoeba and the role of glucosamine-6-P isomerase by RNA interference

Samanta

Ghosh

2012

Molecular and Biochemical Parasitology

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“…Although cyst viability was not evaluated, the fact that only a few cells formed cyst walls, and these were loose and thin as compared to normal cysts (e.g. Chávez-Munguía et al 2007), even employing optimal microscopy procedures, suggests that the encystment maybe not be effectively producing viable cysts.…”

Section: Discussionmentioning

confidence: 99%

Effects of a putrescine analog on Giardia lamblia

et al. 2008

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The protozoan Giardia lamblia is the most frequent intestinal parasite of first-world countries and a major cause of waterborne disorder often referred to as traveler's diarrhea. We have previously noticed that the putrescine analog 1,4-diamino-2-butanone (DAB) remarkably inhibits the growth of anaerobic trichomonad and Trypanosoma cruzi parasites. Here, we examined the role of polyamines in Giardia cells using this putrescine analog. DAB impaired parasite proliferation dose-dependently. The analog induced increased flagella numbers and sometimes four ventral disks as well as asymmetrical division, indicating truncated or deregulated cytokinesis. Electron microscopy analysis revealed that DAB also triggered the encystment process. Oxidative stress was evaluated by measuring lipid peroxidation by thiobarbituric acid reactive substances (TBARS) detection. Trophozoites incubated either with 1 mM of DAB or putrescine for 18 h displayed increased lipoperoxide levels. Addition of 200 microM aminoguanidine, a polyamine/diamine oxidase inhibitor, partially reverted the DAB, but not the putrescine effects, indicating that the DAB effects are due, at least in part, to DAB oxidation end products. These data indicate that polyamines play a role in Giardia cell division, differentiation, and antioxidant defenses.

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“…Besides their role in the cytolytic effect of this parasite, ultrastructural studies have suggested that EDGs may participate in the excystation process in amebas such as E. invadens, A. castellanii, and Naegleria sp (Chá vez-Munguía et al, 2007). …”

Section: Discussionmentioning

confidence: 98%

Entamoeba histolyticaelectrondense granules secretion in vitro and in vivo: Ultrastructural study

Chávez‐Munguía¹,

Castañón²,

Hernández‐Ramírez³

et al. 2011

Microscopy Res & Technique

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Electron dense granules (EDGs) were identified by transmission electron microscopy in Entamoeba histolytica trophozoites recovered from hamster liver lesions. Abundant granules were present in trophozoites recovered after 15 min of liver inoculation. Variation in the size and morphology of these EDGs was also observed. Numerous granules were present in the plasma membrane when these parasites were incubated for 5 min with MDCK monolayers. Release of these EDGs was suggested by the presence of granules in contact with the surface of the target cell plasma membrane. Parasite phagocytic invaginations were observed after 10 min of parasite-monolayer interaction. In these structures, scarce granules were seen. Granules secretion was corroborated by obtaining of a pellet of these small structures from the incubation of trophozoites with collagen supernatant. Collagenase and gellatinase activity of this pellet was identified in SDS-PAGE gels. EDGs were also present in amebic hamster liver lesions. Our observations corroborate that these granules are secreted and suggest that may participate in the cytopathic effect of E. histolytica both in vitro and in vivo.

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Ultrastructure of cyst differentiation in parasitic protozoa

Cited by 41 publications

References 44 publications

The chitin biosynthesis pathway in Entamoeba and the role of glucosamine-6-P isomerase by RNA interference

The chitin biosynthesis pathway in Entamoeba and the role of glucosamine-6-P isomerase by RNA interference

Effects of a putrescine analog on Giardia lamblia

Entamoeba histolyticaelectrondense granules secretion in vitro and in vivo: Ultrastructural study

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