Electron Microscopy for the Diagnosis of Ocular Viral Infections

Boerner, Carol F.; Lee, Francis K.; Wickliffe, Charles W.; Nahmias, André J.; Cavanagh, Harrison D; Straus, Stephen E.

doi:10.1016/s0161-6420(81)34882-9

Cited by 13 publications

(1 citation statement)

References 6 publications

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“…Other reported tests for the direct detection of adenovirus in conjunctival swabs include immunofluorescent antibody [14], immunoperox idase [15], electron microscopy [16] and immunofiltration [17]. Although these tests differ in their sensitivities (range from 38 to 98%), they are all impractical for routine office use.…”

Section: Discussionmentioning

confidence: 99%

Cytological Evaluation of Adenoviral Follicular Conjunctivitis by Cytobrush

Kobayashi¹,

Sato

Tsubota³

et al. 1991

Ophthalmologica

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Conjunctival brush samples from 38 patients with acute conjunctivitis were examined to determine whether cellular features could be utilized for cytodiagnosis. Of all cases, 32 (84%) patients showed a characteristic cell pattern for adenoviral follicular conjunctivitis (AFC) and in the remaining 6 (16%) patients herpetic infection was suggested cytologically. The background of the conjunctival smears from patients with AFC was characterized by the predominance of lymphocytes with little fibrinous discharge. In addition, two types of nuclear alterations were demonstrated i.e. intranuclear inclusions and a so-called ground-glass nuclear appearance. Ground-glass nuclei occurred more frequently than intranuclear inclusions in patients with AFC. However, the presence of lymphocytes in conjunctival smears was a useful criterion for making the differential diagnosis between herpetic and adenoviral infections. Thus, in the absence of these three cellular changes, i.e. ground-glass nuclei, intranuclear inclusions and lymphocytic background, infectious conditions other than AFC may be considered, regardless of the numbers of conjunctival cells present. Emphasis is placed on the value of conjunctival brush cytology for the rapid diagnosis of cases of suspected AFC.

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Section: Discussionmentioning

confidence: 99%